(Linear) (Six-base) MAPSORT of: KIBB8648.seq  Check: 9200  from: 1  to: 1020



KIBB8648.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AccI GT'mk_AC

Cuts at:      0     150     209     536    1020 
   Size:        150      59     327     484

  Fragments arranged by size: 

                 484     327     150      59
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      20      52    1020 
   Size:         20      32     968

  Fragments arranged by size: 

                 968      32      20
 
AlwNI CAG_nnn'CTG

Cuts at:      0     442    1020 
   Size:        442     578
 
ApoI r'AATT_y

Cuts at:      0     319     379    1020 
   Size:        319      60     641

  Fragments arranged by size: 

                 641     319      60
 
BanII G_rGCy'C

Cuts at:      0     174     929    1020 
   Size:        174     755      91

  Fragments arranged by size: 

                 755     174      91
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     943    1020 
   Size:        943      77
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1016    1020 
   Size:       1016       4
 
BsaAI yAC'GTr

Cuts at:      0     574    1020 
   Size:        574     446
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     935    1020 
   Size:        935      85
 
BseYI C'CCAG_C

Cuts at:      0     398    1020 
   Size:        398     622
 
BsiHKAI G_wGCw'C

Cuts at:      0     174     929    1020 
   Size:        174     755      91

  Fragments arranged by size: 

                 755     174      91
 
Bsp1286I G_dGCh'C

Cuts at:      0     174     929    1020 
   Size:        174     755      91

  Fragments arranged by size: 

                 755     174      91
 
BsrDI GCAATG_nn'

Cuts at:      0     186     515    1020 
   Size:        186     329     505

  Fragments arranged by size: 

                 505     329     186
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     991    1020 
   Size:        991      29
 
BstYI r'GATC_y

Cuts at:      0     846    1020 
   Size:        846     174
 
BstZ17I GTA'TAC

Cuts at:      0     151    1020 
   Size:        151     869
 
Bsu36I CC'TnA_GG

Cuts at:      0     508    1020 
   Size:        508     512
 
BtgI C'CryG_G

Cuts at:      0     705    1020 
   Size:        705     315
 
ClaI AT'CG_AT

Cuts at:      0     665    1020 
   Size:        665     355
 
EaeI y'GGCC_r

Cuts at:      0     702     716    1020 
   Size:        702      14     304

  Fragments arranged by size: 

                 702     304      14
 
EarI CTCTTCn'nnn_

Cuts at:      0      41     162     912    1020 
   Size:         41     121     750     108

  Fragments arranged by size: 

                 750     121     108      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      37    1020 
   Size:         37     983
 
EcoICRI GAG'CTC

Cuts at:      0     172     927    1020 
   Size:        172     755      93

  Fragments arranged by size: 

                 755     172      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1016    1020 
   Size:       1016       4
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     486    1020 
   Size:        486     534
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     801     833    1020 
   Size:        801      32     187

  Fragments arranged by size: 

                 801     187      32
 
HindIII A'AGCT_T

Cuts at:      0     368     903    1020 
   Size:        368     535     117

  Fragments arranged by size: 

                 535     368     117
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     442    1020 
   Size:        442     578
 
PfoI T'CCnGG_A

Cuts at:      0     435     819    1020 
   Size:        435     384     201

  Fragments arranged by size: 

                 435     384     201
 
PmlI CAC'GTG

Cuts at:      0     574    1020 
   Size:        574     446
 
SacI G_AGCT'C

Cuts at:      0     174     929    1020 
   Size:        174     755      91

  Fragments arranged by size: 

                 755     174      91
 
SapI GCTCTTCn'nnn_

Cuts at:      0     162    1020 
   Size:        162     858
 
ScaI AGT'ACT

Cuts at:      0     551    1020 
   Size:        551     469
 
SpeI A'CTAG_T

Cuts at:      0     583    1020 
   Size:        583     437
 
StyI C'CwwG_G

Cuts at:      0    1013    1020 
   Size:       1013       7
 
TatI w'GTAC_w

Cuts at:      0     549    1020 
   Size:        549     471
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      83     115    1020 
   Size:         83      32     905

  Fragments arranged by size: 

                 905      83      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     637    1020 
   Size:        637     383
 

 Enzymes that do cut:

     AccI     AloI    AlwNI     ApoI    BanII     BplI     BpmI    BsaAI
    BseRI    BseYI  BsiHKAI Bsp1286I    BsrDI   BstAPI    BstYI  BstZ17I
   Bsu36I     BtgI     ClaI     EaeI     EarI     EciI  EcoICRI  Eco57MI
     FalI    Hin4I  HindIII    PflMI     PfoI     PmlI     SacI     SapI
     ScaI     SpeI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EagI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SphI     SrfI     SspI     StuI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI