Restriction map for
pF1KB8649
(Linear) (Six-base) MAPSORT of: KIBB8649.seq Check: 2423 from: 1 to: 1056
KIBB8649.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 645 699 907 1056
Size: 645 54 208 149
Fragments arranged by size:
645 208 149 54
AccI GT'mk_AC
Cuts at: 0 347 1056
Size: 347 709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 746 817 1056
Size: 415 331 71 239
Fragments arranged by size:
415 331 239 71
AfeI AGC'GCT
Cuts at: 0 859 1056
Size: 859 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 839 1056
Size: 839 217
AlwNI CAG_nnn'CTG
Cuts at: 0 261 519 1056
Size: 261 258 537
Fragments arranged by size:
537 261 258
ApoI r'AATT_y
Cuts at: 0 399 526 1056
Size: 399 127 530
Fragments arranged by size:
530 399 127
AvaI C'yCGr_G
Cuts at: 0 90 732 1056
Size: 90 642 324
Fragments arranged by size:
642 324 90
BanI G'GyrC_C
Cuts at: 0 627 876 1056
Size: 627 249 180
Fragments arranged by size:
627 249 180
BanII G_rGCy'C
Cuts at: 0 420 1056
Size: 420 636
BbsI GAAGACnn'nnnn_
Cuts at: 0 805 1056
Size: 805 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 255 289 1056
Size: 255 34 767
Fragments arranged by size:
767 255 34
BfrBI ATG'CAT
Cuts at: 0 954 1056
Size: 954 102
BlpI GC'TnA_GC
Cuts at: 0 310 967 1056
Size: 310 657 89
Fragments arranged by size:
657 310 89
Bme1580I G_kGCm'C
Cuts at: 0 632 1056
Size: 632 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1056
Size: 889 167
Bpu10I CC'TnA_GC
Cuts at: 0 745 1056
Size: 745 311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1056
Size: 1012 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 172 382 1032 1056
Size: 172 210 650 24
Fragments arranged by size:
650 210 172 24
BsaHI Gr'CG_yC
Cuts at: 0 914 1056
Size: 914 142
BsaWI w'CCGG_w
Cuts at: 0 23 200 1056
Size: 23 177 856
Fragments arranged by size:
856 177 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 690 1024 1056
Size: 484 206 334 32
Fragments arranged by size:
484 334 206 32
BseYI C'CCAG_C
Cuts at: 0 261 850 1056
Size: 261 589 206
Fragments arranged by size:
589 261 206
BsiHKAI G_wGCw'C
Cuts at: 0 677 746 1056
Size: 677 69 310
Fragments arranged by size:
677 310 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1006 1056
Size: 1006 50
Bsp1286I G_dGCh'C
Cuts at: 0 420 632 677 746 1056
Size: 420 212 45 69 310
Fragments arranged by size:
420 310 212 69 45
BspEI T'CCGG_A
Cuts at: 0 23 200 1056
Size: 23 177 856
Fragments arranged by size:
856 177 23
BspHI T'CATG_A
Cuts at: 0 461 1056
Size: 461 595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 645 699 907 1056
Size: 645 54 208 149
Fragments arranged by size:
645 208 149 54
BssSI C'ACGA_G
Cuts at: 0 678 1056
Size: 678 378
BstYI r'GATC_y
Cuts at: 0 722 1056
Size: 722 334
Bsu36I CC'TnA_GG
Cuts at: 0 68 881 1056
Size: 68 813 175
Fragments arranged by size:
813 175 68
BtgI C'CryG_G
Cuts at: 0 434 686 693 1056
Size: 434 252 7 363
Fragments arranged by size:
434 363 252 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1048 1056
Size: 1048 8
DraIII CAC_nnn'GTG
Cuts at: 0 876 1056
Size: 876 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 49 1056
Size: 49 1007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 746 817 889 1056
Size: 415 331 71 72 167
Fragments arranged by size:
415 331 167 72 71
EcoO109I rG'GnC_Cy
Cuts at: 0 149 1056
Size: 149 907
HaeII r_GCGC'y
Cuts at: 0 286 861 1056
Size: 286 575 195
Fragments arranged by size:
575 286 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 988 1020 1056
Size: 988 32 36
Fragments arranged by size:
988 36 32
MslI CAynn'nnrTG
Cuts at: 0 239 1056
Size: 239 817
MspA1I CmG'CkG
Cuts at: 0 81 337 546 695 1056
Size: 81 256 209 149 361
Fragments arranged by size:
361 256 209 149 81
NsiI A_TGCA'T
Cuts at: 0 956 1056
Size: 956 100
NspI r_CATG'y
Cuts at: 0 958 1056
Size: 958 98
PfoI T'CCnGG_A
Cuts at: 0 368 1056
Size: 368 688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 1056
Size: 712 32 312
Fragments arranged by size:
712 312 32
PpuMI rG'GwC_Cy
Cuts at: 0 149 1056
Size: 149 907
PstI C_TGCA'G
Cuts at: 0 668 710 1022 1056
Size: 668 42 312 34
Fragments arranged by size:
668 312 42 34
PvuII CAG'CTG
Cuts at: 0 81 337 546 1056
Size: 81 256 209 510
Fragments arranged by size:
510 256 209 81
SacII CC_GC'GG
Cuts at: 0 696 1056
Size: 696 360
SanDI GG'GwC_CC
Cuts at: 0 149 1056
Size: 149 907
SbfI CC_TGCA'GG
Cuts at: 0 710 1056
Size: 710 346
SfcI C'TryA_G
Cuts at: 0 664 706 942 1018 1056
Size: 664 42 236 76 38
Fragments arranged by size:
664 236 76 42 38
SmaI CCC'GGG
Cuts at: 0 734 1056
Size: 734 322
SmlI C'TyrA_G
Cuts at: 0 1027 1056
Size: 1027 29
SphI G_CATG'C
Cuts at: 0 958 1056
Size: 958 98
StuI AGG'CCT
Cuts at: 0 1003 1056
Size: 1003 53
StyI C'CwwG_G
Cuts at: 0 161 1056
Size: 161 895
TatI w'GTAC_w
Cuts at: 0 131 1056
Size: 131 925
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 667 699 1056
Size: 667 32 357
Fragments arranged by size:
667 357 32
XmaI C'CCGG_G
Cuts at: 0 732 1056
Size: 732 324
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI ApoI AvaI
BanI BanII BbsI BcgI BfrBI BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BssSI BstYI Bsu36I
BtgI BtgZI DraIII DrdI Eco57MI EcoO109I HaeII Hin4I
MslI MspA1I NsiI NspI PfoI PpiI PpuMI PstI
PvuII SacII SanDI SbfI SfcI SmaI SmlI SphI
StuI StyI TatI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BglI BglII BmgBI
BmrI BmtI BplI BsaAI BsaBI BsaXI BsgI BsiEI
BsiWI BsmI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraI
EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TaqII Tth111I XbaI XcmI XhoI XmnI ZraI