Restriction map for
pF1KB8650
(Linear) (Six-base) MAPSORT of: KIBB8650.seq Check: 4495 from: 1 to: 990
KIBB8650.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 386 990
Size: 386 604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 990
Size: 17 973
AhdI GACnn_n'nnGTC
Cuts at: 0 486 990
Size: 486 504
AleI CACnn'nnGTG
Cuts at: 0 509 990
Size: 509 481
AlwNI CAG_nnn'CTG
Cuts at: 0 639 990
Size: 639 351
ApaI G_GGCC'C
Cuts at: 0 828 990
Size: 828 162
ApoI r'AATT_y
Cuts at: 0 144 402 990
Size: 144 258 588
Fragments arranged by size:
588 258 144
AvaI C'yCGr_G
Cuts at: 0 446 827 990
Size: 446 381 163
Fragments arranged by size:
446 381 163
BanI G'GyrC_C
Cuts at: 0 733 975 990
Size: 733 242 15
Fragments arranged by size:
733 242 15
BanII G_rGCy'C
Cuts at: 0 262 828 990
Size: 262 566 162
Fragments arranged by size:
566 262 162
BbsI GAAGACnn'nnnn_
Cuts at: 0 458 990
Size: 458 532
BfrBI ATG'CAT
Cuts at: 0 771 990
Size: 771 219
BglI GCCn_nnn'nGGC
Cuts at: 0 701 990
Size: 701 289
BglII A'GATC_T
Cuts at: 0 425 990
Size: 425 565
Bme1580I G_kGCm'C
Cuts at: 0 140 828 990
Size: 140 688 162
Fragments arranged by size:
688 162 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 784 990
Size: 784 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 396 784 874 990
Size: 83 313 388 90 116
Fragments arranged by size:
388 313 116 90 83
BsaAI yAC'GTr
Cuts at: 0 972 990
Size: 972 18
BsaWI w'CCGG_w
Cuts at: 0 482 548 990
Size: 482 66 442
Fragments arranged by size:
482 442 66
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 713 990
Size: 109 604 277
Fragments arranged by size:
604 277 109
BseYI C'CCAG_C
Cuts at: 0 624 632 867 948 990
Size: 624 8 235 81 42
Fragments arranged by size:
624 235 81 42 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 922 990
Size: 814 108 68
Fragments arranged by size:
814 108 68
BsiEI CG_ry'CG
Cuts at: 0 933 990
Size: 933 57
BsiHKAI G_wGCw'C
Cuts at: 0 262 990
Size: 262 728
BsmI GAATG_Cn'
Cuts at: 0 762 990
Size: 762 228
Bsp1286I G_dGCh'C
Cuts at: 0 140 262 828 990
Size: 140 122 566 162
Fragments arranged by size:
566 162 140 122
BsrFI r'CCGG_y
Cuts at: 0 736 928 990
Size: 736 192 62
Fragments arranged by size:
736 192 62
BstEII G'GTnAC_C
Cuts at: 0 460 990
Size: 460 530
BstXI CCAn_nnnn'nTGG
Cuts at: 0 923 972 990
Size: 923 49 18
Fragments arranged by size:
923 49 18
BstYI r'GATC_y
Cuts at: 0 425 990
Size: 425 565
BtsI GCAGTG_nn'
Cuts at: 0 26 753 990
Size: 26 727 237
Fragments arranged by size:
727 237 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 79 114 990
Size: 79 35 876
Fragments arranged by size:
876 79 35
EaeI y'GGCC_r
Cuts at: 0 930 990
Size: 930 60
EagI C'GGCC_G
Cuts at: 0 930 990
Size: 930 60
EarI CTCTTCn'nnn_
Cuts at: 0 961 990
Size: 961 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 124 990
Size: 124 866
EcoICRI GAG'CTC
Cuts at: 0 260 990
Size: 260 730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 83 396 784 874 990
Size: 17 66 313 388 90 116
Fragments arranged by size:
388 313 116 90 66 17
EcoO109I rG'GnC_Cy
Cuts at: 0 488 849 897 990
Size: 488 361 48 93
Fragments arranged by size:
488 361 93 48
EcoRV GAT'ATC
Cuts at: 0 664 990
Size: 664 326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 444 990
Size: 444 546
HaeII r_GCGC'y
Cuts at: 0 631 835 990
Size: 631 204 155
Fragments arranged by size:
631 204 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 515 547 990
Size: 472 32 11 32 443
Fragments arranged by size:
472 443 32 32 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 558 935 990
Size: 539 19 377 55
Fragments arranged by size:
539 377 55 19
MslI CAynn'nnrTG
Cuts at: 0 296 509 921 990
Size: 296 213 412 69
Fragments arranged by size:
412 296 213 69
MspA1I CmG'CkG
Cuts at: 0 639 881 990
Size: 639 242 109
Fragments arranged by size:
639 242 109
NsiI A_TGCA'T
Cuts at: 0 773 990
Size: 773 217
PasI CC'CwG_GG
Cuts at: 0 265 492 990
Size: 265 227 498
Fragments arranged by size:
498 265 227
PfoI T'CCnGG_A
Cuts at: 0 899 990
Size: 899 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 856 888 990
Size: 856 32 102
Fragments arranged by size:
856 102 32
PpuMI rG'GwC_Cy
Cuts at: 0 488 897 990
Size: 488 409 93
Fragments arranged by size:
488 409 93
PshAI GACnn'nnGTC
Cuts at: 0 458 990
Size: 458 532
PspOMI G'GGCC_C
Cuts at: 0 824 990
Size: 824 166
PstI C_TGCA'G
Cuts at: 0 587 644 671 990
Size: 587 57 27 319
Fragments arranged by size:
587 319 57 27
PvuII CAG'CTG
Cuts at: 0 639 990
Size: 639 351
SacI G_AGCT'C
Cuts at: 0 262 990
Size: 262 728
SfcI C'TryA_G
Cuts at: 0 393 583 640 667 990
Size: 393 190 57 27 323
Fragments arranged by size:
393 323 190 57 27
SmaI CCC'GGG
Cuts at: 0 829 990
Size: 829 161
SrfI GCCC'GGGC
Cuts at: 0 829 990
Size: 829 161
StuI AGG'CCT
Cuts at: 0 185 990
Size: 185 805
XmaI C'CCGG_G
Cuts at: 0 827 990
Size: 827 163
Enzymes that do cut:
AccI AcuI AhdI AleI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BfrBI BglI BglII Bme1580I BmrI
BpmI BsaAI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BsrFI BstEII BstXI BstYI BtsI CspCI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I MmeI MslI MspA1I NsiI PasI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SfcI SmaI SrfI StuI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EcoNI EcoRI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PflMI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI