Restriction map for
pF1KB8651
(Linear) (Six-base) MAPSORT of: KIBB8651.seq Check: 8589 from: 1 to: 1458
KIBB8651.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 672 1458
Size: 672 786
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1458
Size: 655 803
AflII C'TTAA_G
Cuts at: 0 604 1458
Size: 604 854
AhdI GACnn_n'nnGTC
Cuts at: 0 1203 1458
Size: 1203 255
AlwNI CAG_nnn'CTG
Cuts at: 0 53 1008 1458
Size: 53 955 450
Fragments arranged by size:
955 450 53
ApaI G_GGCC'C
Cuts at: 0 189 221 1299 1458
Size: 189 32 1078 159
Fragments arranged by size:
1078 189 159 32
AvaI C'yCGr_G
Cuts at: 0 292 1041 1272 1458
Size: 292 749 231 186
Fragments arranged by size:
749 292 231 186
BanII G_rGCy'C
Cuts at: 0 189 221 1299 1458
Size: 189 32 1078 159
Fragments arranged by size:
1078 189 159 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 1174 1458
Size: 1174 284
BbvCI CC'TCA_GC
Cuts at: 0 401 934 1458
Size: 401 533 524
Fragments arranged by size:
533 524 401
BglI GCCn_nnn'nGGC
Cuts at: 0 250 1458
Size: 250 1208
BlpI GC'TnA_GC
Cuts at: 0 1333 1458
Size: 1333 125
Bme1580I G_kGCm'C
Cuts at: 0 189 221 655 1299 1458
Size: 189 32 434 644 159
Fragments arranged by size:
644 434 189 159 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 216 654 1050 1458
Size: 216 438 396 408
Fragments arranged by size:
438 408 396 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 766 829 1458
Size: 216 550 63 629
Fragments arranged by size:
629 550 216 63
Bpu10I CC'TnA_GC
Cuts at: 0 401 934 1458
Size: 401 533 524
Fragments arranged by size:
533 524 401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1458
Size: 544 914
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 1458
Size: 382 1076
BsaHI Gr'CG_yC
Cuts at: 0 968 1265 1458
Size: 968 297 193
Fragments arranged by size:
968 297 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 20 50 670 700 1204 1234 1458
Size: 20 30 620 30 504 30 224
Fragments arranged by size:
620 504 224 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 724 919 1212 1458
Size: 508 216 195 293 246
Fragments arranged by size:
508 293 246 216 195
BseYI C'CCAG_C
Cuts at: 0 1021 1345 1458
Size: 1021 324 113
Fragments arranged by size:
1021 324 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 271 1458
Size: 132 139 1187
Fragments arranged by size:
1187 139 132
Bsp1286I G_dGCh'C
Cuts at: 0 189 221 655 1299 1458
Size: 189 32 434 644 159
Fragments arranged by size:
644 434 189 159 32
BspHI T'CATG_A
Cuts at: 0 122 1458
Size: 122 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 672 1458
Size: 672 786
BsrBI CCG'CTC
Cuts at: 0 141 1458
Size: 141 1317
BssHII G'CGCG_C
Cuts at: 0 145 1458
Size: 145 1313
BssSI C'ACGA_G
Cuts at: 0 205 512 1458
Size: 205 307 946
Fragments arranged by size:
946 307 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 832 1458
Size: 832 626
Bsu36I CC'TnA_GG
Cuts at: 0 847 1458
Size: 847 611
BtgI C'CryG_G
Cuts at: 0 233 1226 1458
Size: 233 993 232
Fragments arranged by size:
993 233 232
BtsI GCAGTG_nn'
Cuts at: 0 700 842 1458
Size: 700 142 616
Fragments arranged by size:
700 616 142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1424 1458
Size: 1424 34
DraIII CAC_nnn'GTG
Cuts at: 0 182 1458
Size: 182 1276
EaeI y'GGCC_r
Cuts at: 0 99 881 892 1458
Size: 99 782 11 566
Fragments arranged by size:
782 566 99 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 655 766 829 1458
Size: 216 439 111 63 629
Fragments arranged by size:
629 439 216 111 63
EcoO109I rG'GnC_Cy
Cuts at: 0 217 218 843 1296 1458
Size: 217 1 625 453 162
Fragments arranged by size:
625 453 217 162 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 372 619 1419 1458
Size: 372 247 800 39
Fragments arranged by size:
800 372 247 39
FspI TGC'GCA
Cuts at: 0 167 1064 1458
Size: 167 897 394
Fragments arranged by size:
897 394 167
HaeII r_GCGC'y
Cuts at: 0 195 1458
Size: 195 1263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 895 927 1189 1221 1255 1287 1458
Size: 895 32 262 32 34 32 171
Fragments arranged by size:
895 262 171 34 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 1458
Size: 6 1452
MscI TGG'CCA
Cuts at: 0 101 883 1458
Size: 101 782 575
Fragments arranged by size:
782 575 101
MslI CAynn'nnrTG
Cuts at: 0 883 1458
Size: 883 575
MspA1I CmG'CkG
Cuts at: 0 829 1458
Size: 829 629
NdeI CA'TA_TG
Cuts at: 0 12 1458
Size: 12 1446
PasI CC'CwG_GG
Cuts at: 0 431 1458
Size: 431 1027
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 807 890 1190 1458
Size: 666 141 83 300 268
Fragments arranged by size:
666 300 268 141 83
PfoI T'CCnGG_A
Cuts at: 0 731 1458
Size: 731 727
PpuMI rG'GwC_Cy
Cuts at: 0 843 1458
Size: 843 615
PspOMI G'GGCC_C
Cuts at: 0 185 217 1295 1458
Size: 185 32 1078 163
Fragments arranged by size:
1078 185 163 32
PspXI vC'TCGA_Gb
Cuts at: 0 292 1458
Size: 292 1166
PvuII CAG'CTG
Cuts at: 0 829 1458
Size: 829 629
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 250 1458
Size: 250 1208
SmlI C'TyrA_G
Cuts at: 0 292 559 604 1458
Size: 292 267 45 854
Fragments arranged by size:
854 292 267 45
StuI AGG'CCT
Cuts at: 0 244 304 1458
Size: 244 60 1154
Fragments arranged by size:
1154 244 60
StyI C'CwwG_G
Cuts at: 0 392 1458
Size: 392 1066
TatI w'GTAC_w
Cuts at: 0 662 1458
Size: 662 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 20 52 835 867 1458
Size: 20 32 783 32 591
Fragments arranged by size:
783 591 32 32 20
Tth111I GACn'n_nGTC
Cuts at: 0 967 1458
Size: 967 491
XhoI C'TCGA_G
Cuts at: 0 292 1458
Size: 292 1166
XmnI GAAnn'nnTTC
Cuts at: 0 356 1252 1458
Size: 356 896 206
Fragments arranged by size:
896 356 206
Enzymes that do cut:
AarI AcuI AflII AhdI AlwNI ApaI AvaI BanII
BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI Bsp1286I
BspHI BspMI BsrBI BssHII BssSI BstAPI Bsu36I BtgI
BtsI CspCI DraIII EaeI Eco57MI EcoO109I FalI FspI
HaeII Hin4I MmeI MscI MslI MspA1I NdeI PasI
PflMI PfoI PpuMI PspOMI PspXI PvuII SfiI SmlI
StuI StyI TatI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BsaAI BsaBI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI
BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI DraI
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII XbaI XcmI XmaI ZraI