(Linear) (Six-base) MAPSORT of: KIBB8652.seq  Check: 9790  from: 1  to: 603



KIBB8652.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AatII G_ACGT'C

Cuts at:      0     224     603 
   Size:        224     379
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     307     349     603 
   Size:        253      54      42     254

  Fragments arranged by size: 

                 254     253      54      42
 
ApoI r'AATT_y

Cuts at:      0     448     603 
   Size:        448     155
 
BbvCI CC'TCA_GC

Cuts at:      0     590     603 
   Size:        590      13
 
BclI T'GATC_A

Cuts at:      0     522     603 
   Size:        522      81
 
BglII A'GATC_T

Cuts at:      0     290     603 
   Size:        290     313
 
Bme1580I G_kGCm'C

Cuts at:      0     397     603 
   Size:        397     206
 
Bpu10I CC'TnA_GC

Cuts at:      0     101     590     603 
   Size:        101     489      13

  Fragments arranged by size: 

                 489     101      13
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     568     603 
   Size:        568      35
 
BsaHI Gr'CG_yC

Cuts at:      0     221     603 
   Size:        221     382
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     253     586     603 
   Size:        253     333      17

  Fragments arranged by size: 

                 333     253      17
 
BsiEI CG_ry'CG

Cuts at:      0       8     351     603 
   Size:          8     343     252

  Fragments arranged by size: 

                 343     252       8
 
BsmI GAATG_Cn'

Cuts at:      0     435     444     475     603 
   Size:        435       9      31     128

  Fragments arranged by size: 

                 435     128      31       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     397     603 
   Size:        397     206
 
BspHI T'CATG_A

Cuts at:      0      21     603 
   Size:         21     582
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     331     603 
   Size:        331     272
 
BstYI r'GATC_y

Cuts at:      0     290     603 
   Size:        290     313
 
DraI TTT'AAA

Cuts at:      0     147     603 
   Size:        147     456
 
EaeI y'GGCC_r

Cuts at:      0       5     413     603 
   Size:          5     408     190

  Fragments arranged by size: 

                 408     190       5
 
EagI C'GGCC_G

Cuts at:      0       5     603 
   Size:          5     598
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     128     482     603 
   Size:        101      27     354     121

  Fragments arranged by size: 

                 354     121     101      27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     247     556     603 
   Size:        247     309      47

  Fragments arranged by size: 

                 309     247      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     307     349     603 
   Size:        253      54      42     254

  Fragments arranged by size: 

                 254     253      54      42
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     157     603 
   Size:        157     446
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     603 
   Size:         86     517
 
MscI TGG'CCA

Cuts at:      0     415     603 
   Size:        415     188
 
MslI CAynn'nnrTG

Cuts at:      0     316     603 
   Size:        316     287
 
NotI GC'GGCC_GC

Cuts at:      0       5     603 
   Size:          5     598
 
StyI C'CwwG_G

Cuts at:      0      11     578     603 
   Size:         11     567      25

  Fragments arranged by size: 

                 567      25      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     385     603 
   Size:        385     218
 
ZraI GAC'GTC

Cuts at:      0     222     603 
   Size:        222     381
 

 Enzymes that do cut:

    AatII     AcuI     ApoI    BbvCI     BclI    BglII Bme1580I   Bpu10I
     BsaI    BsaHI    BseRI    BsiEI     BsmI Bsp1286I    BspHI    BspMI
    BstYI     DraI     EaeI     EagI     EarI     EciI  Eco57MI     FalI
     MmeI     MscI     MslI     NotI     StyI    TaqII     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI    BfrBI     BglI     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI