Restriction map for
pF1KB8654
(Linear) (Six-base) MAPSORT of: KIBB8654.seq Check: 9972 from: 1 to: 2586
KIBB8654.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 297 1330 2317 2586
Size: 297 1033 987 269
Fragments arranged by size:
1033 987 297 269
AatII G_ACGT'C
Cuts at: 0 166 2586
Size: 166 2420
AccI GT'mk_AC
Cuts at: 0 1790 2586
Size: 1790 796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1281 2458 2586
Size: 1069 212 1177 128
Fragments arranged by size:
1177 1069 212 128
AflIII A'CryG_T
Cuts at: 0 1211 1760 2586
Size: 1211 549 826
Fragments arranged by size:
1211 826 549
AhdI GACnn_n'nnGTC
Cuts at: 0 2559 2586
Size: 2559 27
AleI CACnn'nnGTG
Cuts at: 0 2471 2586
Size: 2471 115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1294 1561 2586
Size: 1294 267 1025
Fragments arranged by size:
1294 1025 267
AlwNI CAG_nnn'CTG
Cuts at: 0 323 549 1743 2318 2427 2586
Size: 323 226 1194 575 109 159
Fragments arranged by size:
1194 575 323 226 159 109
ApaI G_GGCC'C
Cuts at: 0 1310 2224 2586
Size: 1310 914 362
Fragments arranged by size:
1310 914 362
ApaLI G'TGCA_C
Cuts at: 0 1633 2586
Size: 1633 953
AvaI C'yCGr_G
Cuts at: 0 1309 2039 2217 2370 2586
Size: 1309 730 178 153 216
Fragments arranged by size:
1309 730 216 178 153
AvrII C'CTAG_G
Cuts at: 0 498 2586
Size: 498 2088
BanI G'GyrC_C
Cuts at: 0 1595 1966 2586
Size: 1595 371 620
Fragments arranged by size:
1595 620 371
BanII G_rGCy'C
Cuts at: 0 1057 1310 1316 1496 1991 2224 2586
Size: 1057 253 6 180 495 233 362
Fragments arranged by size:
1057 495 362 253 233 180 6
BbeI G_GCGC'C
Cuts at: 0 1599 2586
Size: 1599 987
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 439 1339 2409 2586
Size: 10 429 900 1070 177
Fragments arranged by size:
1070 900 429 177 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1780 1814 2586
Size: 1780 34 772
Fragments arranged by size:
1780 772 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1192 2586
Size: 1192 1394
BglII A'GATC_T
Cuts at: 0 757 2586
Size: 757 1829
BlpI GC'TnA_GC
Cuts at: 0 974 2152 2586
Size: 974 1178 434
Fragments arranged by size:
1178 974 434
Bme1580I G_kGCm'C
Cuts at: 0 1310 1385 1637 2065 2224 2586
Size: 1310 75 252 428 159 362
Fragments arranged by size:
1310 428 362 252 159 75
BmgBI CAC'GTC
Cuts at: 0 1761 2586
Size: 1761 825
BmrI ACTGGGnnnn_n'
Cuts at: 0 134 729 783 1095 2586
Size: 134 595 54 312 1491
Fragments arranged by size:
1491 595 312 134 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 543 2586
Size: 543 2043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1408 1471 2586
Size: 856 552 63 1115
Fragments arranged by size:
1115 856 552 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1588 2586
Size: 1588 998
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 1012 1936 2586
Size: 706 306 924 650
Fragments arranged by size:
924 706 650 306
BsaAI yAC'GTr
Cuts at: 0 180 2586
Size: 180 2406
BsaHI Gr'CG_yC
Cuts at: 0 163 1148 1596 2586
Size: 163 985 448 990
Fragments arranged by size:
990 985 448 163
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1405 2586
Size: 1405 1181
BseYI C'CCAG_C
Cuts at: 0 2160 2586
Size: 2160 426
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 1906 2586
Size: 1706 200 680
Fragments arranged by size:
1706 680 200
BsiHKAI G_wGCw'C
Cuts at: 0 14 1288 1316 1496 1637 1670 1991 2586
Size: 14 1274 28 180 141 33 321 595
Fragments arranged by size:
1274 595 321 180 141 33 28 14
BsmI GAATG_Cn'
Cuts at: 0 571 2586
Size: 571 2015
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2374 2586
Size: 2374 212
Bsp1286I G_dGCh'C
Cuts at: 0 14 1057 1288 1310 1316 1385 1496 1637
Size: 14 1043 231 22 6 69 111 141
Cuts at: 1637 1670 1991 2065 2224 2586
Size: 33 321 74 159 362
Fragments arranged by size:
1043 362 321 231 159 141 111 74
69 33 22 14 6
BspHI T'CATG_A
Cuts at: 0 407 1889 2586
Size: 407 1482 697
Fragments arranged by size:
1482 697 407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 297 563 1286 1330 2317 2586
Size: 297 266 723 44 987 269
Fragments arranged by size:
987 723 297 269 266 44
BsrBI CCG'CTC
Cuts at: 0 978 1592 2192 2586
Size: 978 614 600 394
Fragments arranged by size:
978 614 600 394
BsrDI GCAATG_nn'
Cuts at: 0 1796 2586
Size: 1796 790
BsrFI r'CCGG_y
Cuts at: 0 1150 1598 2586
Size: 1150 448 988
Fragments arranged by size:
1150 988 448
BssSI C'ACGA_G
Cuts at: 0 115 2586
Size: 115 2471
BstAPI GCAn_nnn'nTGC
Cuts at: 0 323 963 1380 2114 2249 2586
Size: 323 640 417 734 135 337
Fragments arranged by size:
734 640 417 337 323 135
BstYI r'GATC_y
Cuts at: 0 757 2586
Size: 757 1829
BtgI C'CryG_G
Cuts at: 0 257 420 488 1009 1132 1253 2469 2586
Size: 257 163 68 521 123 121 1216 117
Fragments arranged by size:
1216 521 257 163 123 121 117 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1233 1952 2390 2586
Size: 1233 719 438 196
Fragments arranged by size:
1233 719 438 196
BtsI GCAGTG_nn'
Cuts at: 0 1689 1694 1923 1932 2265 2319 2586
Size: 1689 5 229 9 333 54 267
Fragments arranged by size:
1689 333 267 229 54 9 5
DraI TTT'AAA
Cuts at: 0 821 2586
Size: 821 1765
DraIII CAC_nnn'GTG
Cuts at: 0 1870 2289 2586
Size: 1870 419 297
Fragments arranged by size:
1870 419 297
EaeI y'GGCC_r
Cuts at: 0 138 926 1844 2169 2570 2586
Size: 138 788 918 325 401 16
Fragments arranged by size:
918 788 401 325 138 16
EarI CTCTTCn'nnn_
Cuts at: 0 939 1321 2586
Size: 939 382 1265
Fragments arranged by size:
1265 939 382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2289 2586
Size: 2289 297
EcoICRI GAG'CTC
Cuts at: 0 1314 1494 1989 2586
Size: 1314 180 495 597
Fragments arranged by size:
1314 597 495 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1069 1281 1408 1471 2458 2586
Size: 856 213 212 127 63 987 128
Fragments arranged by size:
987 856 213 212 128 127 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1483 2586
Size: 1483 1103
EcoO109I rG'GnC_Cy
Cuts at: 0 101 128 890 1740 2221 2519 2586
Size: 101 27 762 850 481 298 67
Fragments arranged by size:
850 762 481 298 101 67 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 51 2586
Size: 51 2535
HaeII r_GCGC'y
Cuts at: 0 526 1599 2586
Size: 526 1073 987
Fragments arranged by size:
1073 987 526
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1232 1264 2200 2232 2545 2577 2586
Size: 1232 32 936 32 313 32 9
Fragments arranged by size:
1232 936 313 32 32 32 9
HincII GTy'rAC
Cuts at: 0 741 1791 2586
Size: 741 1050 795
Fragments arranged by size:
1050 795 741
HpaI GTT'AAC
Cuts at: 0 741 2586
Size: 741 1845
KasI G'GCGC_C
Cuts at: 0 1595 2586
Size: 1595 991
MfeI C'AATT_G
Cuts at: 0 47 2586
Size: 47 2539
MluI A'CGCG_T
Cuts at: 0 1211 2586
Size: 1211 1375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 2586
Size: 682 1904
MscI TGG'CCA
Cuts at: 0 140 928 2586
Size: 140 788 1658
Fragments arranged by size:
1658 788 140
MslI CAynn'nnrTG
Cuts at: 0 1174 1504 1543 1937 2471 2586
Size: 1174 330 39 394 534 115
Fragments arranged by size:
1174 534 394 330 115 39
MspA1I CmG'CkG
Cuts at: 0 1134 1413 1701 1994 2058 2471 2586
Size: 1134 279 288 293 64 413 115
Fragments arranged by size:
1134 413 293 288 279 115 64
NaeI GCC'GGC
Cuts at: 0 1152 1600 2586
Size: 1152 448 986
Fragments arranged by size:
1152 986 448
NarI GG'CG_CC
Cuts at: 0 1596 2586
Size: 1596 990
NcoI C'CATG_G
Cuts at: 0 420 488 2586
Size: 420 68 2098
Fragments arranged by size:
2098 420 68
NgoMIV G'CCGG_C
Cuts at: 0 1150 1598 2586
Size: 1150 448 988
Fragments arranged by size:
1150 988 448
NspI r_CATG'y
Cuts at: 0 2160 2446 2586
Size: 2160 286 140
Fragments arranged by size:
2160 286 140
PasI CC'CwG_GG
Cuts at: 0 663 1716 2524 2586
Size: 663 1053 808 62
Fragments arranged by size:
1053 808 663 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 549 1743 1755 2178 2427 2586
Size: 549 1194 12 423 249 159
Fragments arranged by size:
1194 549 423 249 159 12
PfoI T'CCnGG_A
Cuts at: 0 1327 2420 2586
Size: 1327 1093 166
Fragments arranged by size:
1327 1093 166
PmlI CAC'GTG
Cuts at: 0 180 2586
Size: 180 2406
PpuMI rG'GwC_Cy
Cuts at: 0 128 1740 2586
Size: 128 1612 846
Fragments arranged by size:
1612 846 128
PshAI GACnn'nnGTC
Cuts at: 0 166 2586
Size: 166 2420
PspOMI G'GGCC_C
Cuts at: 0 1306 2220 2586
Size: 1306 914 366
Fragments arranged by size:
1306 914 366
PstI C_TGCA'G
Cuts at: 0 308 731 1283 2314 2586
Size: 308 423 552 1031 272
Fragments arranged by size:
1031 552 423 308 272
PvuII CAG'CTG
Cuts at: 0 1701 2586
Size: 1701 885
SacI G_AGCT'C
Cuts at: 0 1316 1496 1991 2586
Size: 1316 180 495 595
Fragments arranged by size:
1316 595 495 180
SacII CC_GC'GG
Cuts at: 0 1135 2472 2586
Size: 1135 1337 114
Fragments arranged by size:
1337 1135 114
SalI G'TCGA_C
Cuts at: 0 1789 2586
Size: 1789 797
SapI GCTCTTCn'nnn_
Cuts at: 0 939 1321 2586
Size: 939 382 1265
Fragments arranged by size:
1265 939 382
SbfI CC_TGCA'GG
Cuts at: 0 308 2586
Size: 308 2278
SexAI A'CCwGG_T
Cuts at: 0 577 2586
Size: 577 2009
SfcI C'TryA_G
Cuts at: 0 304 727 1279 2310 2586
Size: 304 423 552 1031 276
Fragments arranged by size:
1031 552 423 304 276
SfoI GGC'GCC
Cuts at: 0 1597 2586
Size: 1597 989
SgrAI Cr'CCGG_yG
Cuts at: 0 1150 2586
Size: 1150 1436
SmaI CCC'GGG
Cuts at: 0 2372 2586
Size: 2372 214
SmlI C'TyrA_G
Cuts at: 0 1603 2586
Size: 1603 983
SphI G_CATG'C
Cuts at: 0 2160 2446 2586
Size: 2160 286 140
Fragments arranged by size:
2160 286 140
SspI AAT'ATT
Cuts at: 0 595 2586
Size: 595 1991
StyI C'CwwG_G
Cuts at: 0 35 420 488 498 734 2549 2586
Size: 35 385 68 10 236 1815 37
Fragments arranged by size:
1815 385 236 68 37 35 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 711 794 1039 2096 2586
Size: 711 83 245 1057 490
Fragments arranged by size:
1057 711 490 245 83
TatI w'GTAC_w
Cuts at: 0 119 1229 1895 2586
Size: 119 1110 666 691
Fragments arranged by size:
1110 691 666 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 597 629 659 691 2586
Size: 597 32 30 32 1895
Fragments arranged by size:
1895 597 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 0 2413 2586
Size: 2413 173
XbaI T'CTAG_A
Cuts at: 0 64 2586
Size: 64 2522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 121 288 716 936 1803 2232 2586
Size: 121 167 428 220 867 429 354
Fragments arranged by size:
867 429 428 354 220 167 121
XmaI C'CCGG_G
Cuts at: 0 2370 2586
Size: 2370 216
ZraI GAC'GTC
Cuts at: 0 164 2586
Size: 164 2422
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI
BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaHI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstYI BtgI BtgZI BtsI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HpaI KasI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SalI SapI SbfI
SexAI SfcI SfoI SgrAI SmaI SmlI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AloI ApoI AscI
AseI AsiSI BaeI BamHI BbvCI BciVI BclI BfrBI
BmtI Bpu10I BsaBI BsaWI BsaXI BsiEI BsiWI BspEI
BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DrdI EagI EcoRI EcoRV FseI FspI FspAI
HindIII KpnI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SanDI ScaI SfiI SgfI SnaBI SpeI SrfI StuI
SwaI XhoI XmnI