Restriction map for
pF1KB8656
(Linear) (Six-base) MAPSORT of: KIBB8656.seq Check: 7982 from: 1 to: 1701
KIBB8656.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 693 1142 1701
Size: 693 449 559
Fragments arranged by size:
693 559 449
AccI GT'mk_AC
Cuts at: 0 101 384 1154 1701
Size: 101 283 770 547
Fragments arranged by size:
770 547 283 101
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 898 973 1701
Size: 347 551 75 728
Fragments arranged by size:
728 551 347 75
AflIII A'CryG_T
Cuts at: 0 305 1701
Size: 305 1396
AgeI A'CCGG_T
Cuts at: 0 536 1701
Size: 536 1165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 120 1701
Size: 120 1581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 418 450 1701
Size: 418 32 1251
Fragments arranged by size:
1251 418 32
ApaI G_GGCC'C
Cuts at: 0 473 1701
Size: 473 1228
ApoI r'AATT_y
Cuts at: 0 528 1034 1239 1372 1701
Size: 528 506 205 133 329
Fragments arranged by size:
528 506 329 205 133
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 985 1018 1701
Size: 985 33 683
Fragments arranged by size:
985 683 33
BanI G'GyrC_C
Cuts at: 0 1332 1701
Size: 1332 369
BanII G_rGCy'C
Cuts at: 0 278 473 487 495 1701
Size: 278 195 14 8 1206
Fragments arranged by size:
1206 278 195 14 8
Bme1580I G_kGCm'C
Cuts at: 0 473 1701
Size: 473 1228
BmrI ACTGGGnnnn_n'
Cuts at: 0 560 1701
Size: 560 1141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 184 444 1197 1701
Size: 184 260 753 504
Fragments arranged by size:
753 504 260 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 509 1596 1701
Size: 414 95 1087 105
Fragments arranged by size:
1087 414 105 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 1701
Size: 96 1605
BsaBI GATnn'nnATC
Cuts at: 0 333 1469 1701
Size: 333 1136 232
Fragments arranged by size:
1136 333 232
BsaWI w'CCGG_w
Cuts at: 0 536 1701
Size: 536 1165
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 617 1701
Size: 617 1084
BseYI C'CCAG_C
Cuts at: 0 1234 1701
Size: 1234 467
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1149 1701
Size: 1149 552
BsiHKAI G_wGCw'C
Cuts at: 0 82 487 1701
Size: 82 405 1214
Fragments arranged by size:
1214 405 82
BsmI GAATG_Cn'
Cuts at: 0 918 1401 1701
Size: 918 483 300
Fragments arranged by size:
918 483 300
Bsp1286I G_dGCh'C
Cuts at: 0 82 278 473 487 495 1701
Size: 82 196 195 14 8 1206
Fragments arranged by size:
1206 196 195 82 14 8
BspHI T'CATG_A
Cuts at: 0 1635 1701
Size: 1635 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 693 1142 1701
Size: 693 449 559
Fragments arranged by size:
693 559 449
BsrDI GCAATG_nn'
Cuts at: 0 227 941 1701
Size: 227 714 760
Fragments arranged by size:
760 714 227
BsrFI r'CCGG_y
Cuts at: 0 536 1701
Size: 536 1165
BstBI TT'CG_AA
Cuts at: 0 556 1701
Size: 556 1145
BstYI r'GATC_y
Cuts at: 0 573 1701
Size: 573 1128
Bsu36I CC'TnA_GG
Cuts at: 0 279 1701
Size: 279 1422
DraI TTT'AAA
Cuts at: 0 66 638 753 1701
Size: 66 572 115 948
Fragments arranged by size:
948 572 115 66
DraIII CAC_nnn'GTG
Cuts at: 0 190 568 1701
Size: 190 378 1133
Fragments arranged by size:
1133 378 190
EarI CTCTTCn'nnn_
Cuts at: 0 153 366 917 1701
Size: 153 213 551 784
Fragments arranged by size:
784 551 213 153
EcoICRI GAG'CTC
Cuts at: 0 485 1701
Size: 485 1216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 414 509 898 973 1596 1701
Size: 347 67 95 389 75 623 105
Fragments arranged by size:
623 389 347 105 95 75 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 342 869 927 1701
Size: 342 527 58 774
Fragments arranged by size:
774 527 342 58
EcoO109I rG'GnC_Cy
Cuts at: 0 470 1701
Size: 470 1231
EcoRI G'AATT_C
Cuts at: 0 1239 1701
Size: 1239 462
HincII GTy'rAC
Cuts at: 0 1297 1701
Size: 1297 404
HindIII A'AGCT_T
Cuts at: 0 747 1701
Size: 747 954
MfeI C'AATT_G
Cuts at: 0 613 1701
Size: 613 1088
MslI CAynn'nnrTG
Cuts at: 0 1669 1701
Size: 1669 32
MspA1I CmG'CkG
Cuts at: 0 772 1701
Size: 772 929
NdeI CA'TA_TG
Cuts at: 0 834 1701
Size: 834 867
NspI r_CATG'y
Cuts at: 0 309 1144 1701
Size: 309 835 557
Fragments arranged by size:
835 557 309
PciI A'CATG_T
Cuts at: 0 305 1701
Size: 305 1396
PsiI TTA'TAA
Cuts at: 0 236 1391 1417 1701
Size: 236 1155 26 284
Fragments arranged by size:
1155 284 236 26
PspOMI G'GGCC_C
Cuts at: 0 469 1701
Size: 469 1232
PstI C_TGCA'G
Cuts at: 0 99 1701
Size: 99 1602
PvuII CAG'CTG
Cuts at: 0 772 1701
Size: 772 929
SacI G_AGCT'C
Cuts at: 0 487 1701
Size: 487 1214
SapI GCTCTTCn'nnn_
Cuts at: 0 917 1701
Size: 917 784
SfcI C'TryA_G
Cuts at: 0 95 1701
Size: 95 1606
SmlI C'TyrA_G
Cuts at: 0 75 1701
Size: 75 1626
SpeI A'CTAG_T
Cuts at: 0 351 1701
Size: 351 1350
StyI C'CwwG_G
Cuts at: 0 928 1701
Size: 928 773
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 579 1701
Size: 579 1122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 399 431 1701
Size: 399 32 1270
Fragments arranged by size:
1270 399 32
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AlfI AloI ApaI
ApoI BaeI BanI BanII Bme1580I BmrI BplI BpmI
BpuEI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrFI BstBI BstYI Bsu36I
DraI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HincII HindIII MfeI MslI MspA1I NdeI NspI PciI
PsiI PspOMI PstI PvuII SacI SapI SfcI SmlI
SpeI StyI TaqII TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AleI AlwNI
ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI Bpu10I BsaI BsaAI BsaHI BsaXI
BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DrdI EaeI EagI EciI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HpaI KasI KpnI MluI
MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI