Restriction map for
pF1KB8658
(Linear) (Six-base) MAPSORT of: KIBB8658.seq Check: 2307 from: 1 to: 1560
KIBB8658.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1560
Size: 1560
AatII G_ACGT'C
Cuts at: 0 1428 1560
Size: 1428 132
AccI GT'mk_AC
Cuts at: 0 371 824 1422 1560
Size: 371 453 598 138
Fragments arranged by size:
598 453 371 138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1560
Size: 839 721
AlwNI CAG_nnn'CTG
Cuts at: 0 304 1560
Size: 304 1256
AseI AT'TA_AT
Cuts at: 0 395 1560
Size: 395 1165
BanII G_rGCy'C
Cuts at: 0 870 1560
Size: 870 690
BbsI GAAGACnn'nnnn_
Cuts at: 0 1397 1560
Size: 1397 163
BciVI GTATCCnnnnn_n'
Cuts at: 0 530 1560
Size: 530 1030
BclI T'GATC_A
Cuts at: 0 1245 1560
Size: 1245 315
BglII A'GATC_T
Cuts at: 0 808 1560
Size: 808 752
BlpI GC'TnA_GC
Cuts at: 0 37 1560
Size: 37 1523
BmrI ACTGGGnnnn_n'
Cuts at: 0 692 1560
Size: 692 868
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 424 1560
Size: 424 1136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 1560
Size: 845 715
BsaI GGTCTCn'nnnn_
Cuts at: 0 187 1184 1560
Size: 187 997 376
Fragments arranged by size:
997 376 187
BsaAI yAC'GTr
Cuts at: 0 1042 1560
Size: 1042 518
BsaHI Gr'CG_yC
Cuts at: 0 1425 1560
Size: 1425 135
BsaWI w'CCGG_w
Cuts at: 0 49 1560
Size: 49 1511
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 279 309 648 678 1560
Size: 55 30 194 30 339 30 882
Fragments arranged by size:
882 339 194 55 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 653 860 881 899 950 1560
Size: 76 577 207 21 18 51 610
Fragments arranged by size:
610 577 207 76 51 21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1560
Size: 1022 538
BsiEI CG_ry'CG
Cuts at: 0 8 1560
Size: 8 1552
BsiHKAI G_wGCw'C
Cuts at: 0 276 870 1540 1560
Size: 276 594 670 20
Fragments arranged by size:
670 594 276 20
Bsp1286I G_dGCh'C
Cuts at: 0 276 870 1540 1560
Size: 276 594 670 20
Fragments arranged by size:
670 594 276 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1560
Size: 1560
BsrBI CCG'CTC
Cuts at: 0 41 1560
Size: 41 1519
BsrDI GCAATG_nn'
Cuts at: 0 1065 1560
Size: 1065 495
BsrFI r'CCGG_y
Cuts at: 0 7 1560
Size: 7 1553
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1081 1560
Size: 1081 479
BstYI r'GATC_y
Cuts at: 0 808 1560
Size: 808 752
BtgI C'CryG_G
Cuts at: 0 445 1560
Size: 445 1115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 98 236 1292 1560
Size: 98 138 1056 268
Fragments arranged by size:
1056 268 138 98
BtsI GCAGTG_nn'
Cuts at: 0 923 1132 1518 1560
Size: 923 209 386 42
Fragments arranged by size:
923 386 209 42
DraIII CAC_nnn'GTG
Cuts at: 0 1264 1560
Size: 1264 296
EaeI y'GGCC_r
Cuts at: 0 5 1560
Size: 5 1555
EagI C'GGCC_G
Cuts at: 0 5 1560
Size: 5 1555
EarI CTCTTCn'nnn_
Cuts at: 0 62 327 1140 1560
Size: 62 265 813 420
Fragments arranged by size:
813 420 265 62
EcoICRI GAG'CTC
Cuts at: 0 868 1560
Size: 868 692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 845 1560
Size: 839 6 715
Fragments arranged by size:
839 715 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 965 1560
Size: 965 595
EcoO109I rG'GnC_Cy
Cuts at: 0 795 1560
Size: 795 765
HaeII r_GCGC'y
Cuts at: 0 24 974 1560
Size: 24 950 586
Fragments arranged by size:
950 586 24
HindIII A'AGCT_T
Cuts at: 0 512 1560
Size: 512 1048
MfeI C'AATT_G
Cuts at: 0 716 1560
Size: 716 844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 937 1560
Size: 11 926 623
Fragments arranged by size:
926 623 11
MslI CAynn'nnrTG
Cuts at: 0 1436 1560
Size: 1436 124
NaeI GCC'GGC
Cuts at: 0 9 1560
Size: 9 1551
NcoI C'CATG_G
Cuts at: 0 445 1560
Size: 445 1115
NdeI CA'TA_TG
Cuts at: 0 1053 1560
Size: 1053 507
NgoMIV G'CCGG_C
Cuts at: 0 7 1560
Size: 7 1553
PflMI CCAn_nnn'nTGG
Cuts at: 0 283 1560
Size: 283 1277
PfoI T'CCnGG_A
Cuts at: 0 861 1560
Size: 861 699
PmlI CAC'GTG
Cuts at: 0 1042 1560
Size: 1042 518
PpuMI rG'GwC_Cy
Cuts at: 0 795 1560
Size: 795 765
PshAI GACnn'nnGTC
Cuts at: 0 358 1401 1560
Size: 358 1043 159
Fragments arranged by size:
1043 358 159
PstI C_TGCA'G
Cuts at: 0 1557 1560
Size: 1557 3
SacI G_AGCT'C
Cuts at: 0 870 1560
Size: 870 690
SexAI A'CCwGG_T
Cuts at: 0 456 1560
Size: 456 1104
SfcI C'TryA_G
Cuts at: 0 251 347 1553 1560
Size: 251 96 1206 7
Fragments arranged by size:
1206 251 96 7
StyI C'CwwG_G
Cuts at: 0 445 1560
Size: 445 1115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 718 1172 1560
Size: 211 507 454 388
Fragments arranged by size:
507 454 388 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 867 899 1560
Size: 55 32 780 32 661
Fragments arranged by size:
780 661 55 32 32
ZraI GAC'GTC
Cuts at: 0 1426 1560
Size: 1426 134
Enzymes that do cut:
AarI AatII AccI AcuI AlwNI AseI BanII BbsI
BciVI BclI BglII BlpI BmrI BplI BpmI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII HindIII MfeI MmeI MslI NaeI
NcoI NdeI NgoMIV PflMI PfoI PmlI PpuMI PshAI
PstI SacI SexAI SfcI StyI TaqII TstI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI
BglI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaBI BseYI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HpaI KasI KpnI MluI MscI MspA1I
NarI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI