Restriction map for
pF1KB8660
(Linear) (Six-base) MAPSORT of: KIBB8660.seq Check: 7693 from: 1 to: 1647
KIBB8660.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AatII G_ACGT'C
Cuts at: 0 946 1647
Size: 946 701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 1539 1647
Size: 459 1080 108
Fragments arranged by size:
1080 459 108
AflIII A'CryG_T
Cuts at: 0 860 1647
Size: 860 787
AgeI A'CCGG_T
Cuts at: 0 977 1647
Size: 977 670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 987 1647
Size: 987 660
AlwNI CAG_nnn'CTG
Cuts at: 0 639 888 1647
Size: 639 249 759
Fragments arranged by size:
759 639 249
ApaI G_GGCC'C
Cuts at: 0 325 1399 1647
Size: 325 1074 248
Fragments arranged by size:
1074 325 248
ApoI r'AATT_y
Cuts at: 0 200 1647
Size: 200 1447
AvaI C'yCGr_G
Cuts at: 0 835 1087 1647
Size: 835 252 560
Fragments arranged by size:
835 560 252
BanI G'GyrC_C
Cuts at: 0 1224 1546 1647
Size: 1224 322 101
Fragments arranged by size:
1224 322 101
BanII G_rGCy'C
Cuts at: 0 325 704 842 1399 1647
Size: 325 379 138 557 248
Fragments arranged by size:
557 379 325 248 138
BbsI GAAGACnn'nnnn_
Cuts at: 0 946 1144 1274 1647
Size: 946 198 130 373
Fragments arranged by size:
946 373 198 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 963 997 1647
Size: 963 34 650
Fragments arranged by size:
963 650 34
BfrBI ATG'CAT
Cuts at: 0 616 1647
Size: 616 1031
BglI GCCn_nnn'nGGC
Cuts at: 0 683 1607 1647
Size: 683 924 40
Fragments arranged by size:
924 683 40
BglII A'GATC_T
Cuts at: 0 771 1647
Size: 771 876
Bme1580I G_kGCm'C
Cuts at: 0 36 325 627 966 1227 1288 1301 1399
Size: 36 289 302 339 261 61 13 98
Cuts at: 1399 1549 1647
Size: 150 98
Fragments arranged by size:
339 302 289 261 150 98 98 61
36 13
BmgBI CAC'GTC
Cuts at: 0 6 734 861 1647
Size: 6 728 127 786
Fragments arranged by size:
786 728 127 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 261 1647
Size: 261 1386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 672 1647
Size: 672 975
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1154 1359 1622 1647
Size: 1154 205 263 25
Fragments arranged by size:
1154 263 205 25
Bpu10I CC'TnA_GC
Cuts at: 0 107 568 1316 1647
Size: 107 461 748 331
Fragments arranged by size:
748 461 331 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1647
Size: 1003 644
BsaAI yAC'GTr
Cuts at: 0 1129 1647
Size: 1129 518
BsaHI Gr'CG_yC
Cuts at: 0 943 1647
Size: 943 704
BsaWI w'CCGG_w
Cuts at: 0 809 977 1647
Size: 809 168 670
Fragments arranged by size:
809 670 168
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 547 583 622 1647
Size: 164 383 36 39 1025
Fragments arranged by size:
1025 383 164 39 36
BseYI C'CCAG_C
Cuts at: 0 82 243 1077 1300 1433 1525 1647
Size: 82 161 834 223 133 92 122
Fragments arranged by size:
834 223 161 133 122 92 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1505 1647
Size: 652 853 142
Fragments arranged by size:
853 652 142
BsiEI CG_ry'CG
Cuts at: 0 438 1647
Size: 438 1209
BsiHKAI G_wGCw'C
Cuts at: 0 422 1019 1584 1647
Size: 422 597 565 63
Fragments arranged by size:
597 565 422 63
BsmI GAATG_Cn'
Cuts at: 0 308 616 1647
Size: 308 308 1031
Fragments arranged by size:
1031 308 308
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 1647
Size: 154 1493
Bsp1286I G_dGCh'C
Cuts at: 0 36 325 422 627 704 842 966 1019
Size: 36 289 97 205 77 138 124 53
Cuts at: 1019 1227 1288 1301 1399 1549 1584 1647
Size: 208 61 13 98 150 35 63
Fragments arranged by size:
289 208 205 150 138 124 98 97
77 63 61 53 36 35 13
BspEI T'CCGG_A
Cuts at: 0 809 1647
Size: 809 838
BspHI T'CATG_A
Cuts at: 0 389 1647
Size: 389 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1076 1647
Size: 1076 571
BsrBI CCG'CTC
Cuts at: 0 1500 1647
Size: 1500 147
BsrFI r'CCGG_y
Cuts at: 0 977 1119 1647
Size: 977 142 528
Fragments arranged by size:
977 528 142
BsrGI T'GTAC_A
Cuts at: 0 153 1647
Size: 153 1494
BstAPI GCAn_nnn'nTGC
Cuts at: 0 660 1647
Size: 660 987
BstBI TT'CG_AA
Cuts at: 0 117 1647
Size: 117 1530
BstXI CCAn_nnnn'nTGG
Cuts at: 0 750 1647
Size: 750 897
BstYI r'GATC_y
Cuts at: 0 771 827 1647
Size: 771 56 820
Fragments arranged by size:
820 771 56
Bsu36I CC'TnA_GG
Cuts at: 0 605 1328 1590 1647
Size: 605 723 262 57
Fragments arranged by size:
723 605 262 57
BtgI C'CryG_G
Cuts at: 0 749 958 1192 1219 1647
Size: 749 209 234 27 428
Fragments arranged by size:
749 428 234 209 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 561 1647
Size: 561 1086
BtsI GCAGTG_nn'
Cuts at: 0 790 1357 1582 1647
Size: 790 567 225 65
Fragments arranged by size:
790 567 225 65
ClaI AT'CG_AT
Cuts at: 0 483 1647
Size: 483 1164
EaeI y'GGCC_r
Cuts at: 0 641 1647
Size: 641 1006
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1396 1647
Size: 1396 251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 1154 1359 1539 1622 1647
Size: 459 695 205 180 83 25
Fragments arranged by size:
695 459 205 180 83 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 996 1147 1315 1647
Size: 996 151 168 332
Fragments arranged by size:
996 332 168 151
EcoO109I rG'GnC_Cy
Cuts at: 0 103 321 564 1031 1065 1164 1324 1395
Size: 103 218 243 467 34 99 160 71
Cuts at: 1395 1396 1594 1647
Size: 1 198 53
Fragments arranged by size:
467 243 218 198 160 103 99 71
53 34 1
HaeII r_GCGC'y
Cuts at: 0 889 933 1647
Size: 889 44 714
Fragments arranged by size:
889 714 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 371 403 814 846 1081 1113
Size: 35 32 304 32 411 32 235 32
Cuts at: 1113 1647
Size: 534
Fragments arranged by size:
534 411 304 235 35 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1562 1647
Size: 1562 85
MscI TGG'CCA
Cuts at: 0 643 1647
Size: 643 1004
MslI CAynn'nnrTG
Cuts at: 0 55 1647
Size: 55 1592
MspA1I CmG'CkG
Cuts at: 0 501 636 1194 1464 1647
Size: 501 135 558 270 183
Fragments arranged by size:
558 501 270 183 135
NaeI GCC'GGC
Cuts at: 0 1121 1647
Size: 1121 526
NcoI C'CATG_G
Cuts at: 0 749 1647
Size: 749 898
NdeI CA'TA_TG
Cuts at: 0 528 1647
Size: 528 1119
NgoMIV G'CCGG_C
Cuts at: 0 1119 1647
Size: 1119 528
NsiI A_TGCA'T
Cuts at: 0 618 1647
Size: 618 1029
NspI r_CATG'y
Cuts at: 0 724 1647
Size: 724 923
PfoI T'CCnGG_A
Cuts at: 0 1262 1336 1647
Size: 1262 74 311
Fragments arranged by size:
1262 311 74
PmlI CAC'GTG
Cuts at: 0 1129 1647
Size: 1129 518
PpuMI rG'GwC_Cy
Cuts at: 0 564 1065 1324 1647
Size: 564 501 259 323
Fragments arranged by size:
564 501 323 259
PspOMI G'GGCC_C
Cuts at: 0 321 1395 1647
Size: 321 1074 252
Fragments arranged by size:
1074 321 252
PstI C_TGCA'G
Cuts at: 0 562 665 1352 1647
Size: 562 103 687 295
Fragments arranged by size:
687 562 295 103
PvuII CAG'CTG
Cuts at: 0 636 1464 1647
Size: 636 828 183
Fragments arranged by size:
828 636 183
SacII CC_GC'GG
Cuts at: 0 1195 1647
Size: 1195 452
SbfI CC_TGCA'GG
Cuts at: 0 665 1647
Size: 665 982
SfcI C'TryA_G
Cuts at: 0 558 661 1348 1647
Size: 558 103 687 299
Fragments arranged by size:
687 558 299 103
SmlI C'TyrA_G
Cuts at: 0 1018 1647
Size: 1018 629
StuI AGG'CCT
Cuts at: 0 1113 1647
Size: 1113 534
StyI C'CwwG_G
Cuts at: 0 749 1399 1647
Size: 749 650 248
Fragments arranged by size:
749 650 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 1539 1647
Size: 222 1317 108
Fragments arranged by size:
1317 222 108
TatI w'GTAC_w
Cuts at: 0 153 536 1647
Size: 153 383 1111
Fragments arranged by size:
1111 383 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1230 1262 1647
Size: 1230 32 385
Fragments arranged by size:
1230 385 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1308 1647
Size: 1308 339
ZraI GAC'GTC
Cuts at: 0 944 1647
Size: 944 703
Enzymes that do cut:
AatII AcuI AflIII AgeI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BcgI BfrBI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI EaeI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NsiI NspI PfoI PmlI PpuMI PspOMI PstI PvuII
SacII SbfI SfcI SmlI StuI StyI TaqII TatI
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BciVI BclI BlpI BmtI BsaI BsaBI
BsaXI BsiWI BsrDI BssHII BssSI BstEII BstZ17I CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NheI NotI NruI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
XmnI