Restriction map for
pF1KB8661
(Linear) (Six-base) MAPSORT of: KIBB8661.seq Check: 1197 from: 1 to: 1260
KIBB8661.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1260
Size: 224 1036
AflIII A'CryG_T
Cuts at: 0 76 1145 1260
Size: 76 1069 115
Fragments arranged by size:
1069 115 76
AleI CACnn'nnGTG
Cuts at: 0 787 1260
Size: 787 473
AlwNI CAG_nnn'CTG
Cuts at: 0 961 1260
Size: 961 299
BanI G'GyrC_C
Cuts at: 0 170 1260
Size: 170 1090
BanII G_rGCy'C
Cuts at: 0 1002 1188 1236 1260
Size: 1002 186 48 24
Fragments arranged by size:
1002 186 48 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 205 1260
Size: 205 1055
BclI T'GATC_A
Cuts at: 0 582 1260
Size: 582 678
Bme1580I G_kGCm'C
Cuts at: 0 175 1260
Size: 175 1085
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 1260
Size: 51 1209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1055 1172 1260
Size: 197 858 117 88
Fragments arranged by size:
858 197 117 88
BsaWI w'CCGG_w
Cuts at: 0 773 1260
Size: 773 487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 478 508 1260
Size: 478 30 752
Fragments arranged by size:
752 478 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 539 998 1001 1260
Size: 539 459 3 259
Fragments arranged by size:
539 459 259 3
BseYI C'CCAG_C
Cuts at: 0 408 1260
Size: 408 852
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1260
Size: 811 449
BsiHKAI G_wGCw'C
Cuts at: 0 1002 1236 1260
Size: 1002 234 24
Fragments arranged by size:
1002 234 24
BsmI GAATG_Cn'
Cuts at: 0 136 1260
Size: 136 1124
Bsp1286I G_dGCh'C
Cuts at: 0 175 1002 1188 1236 1260
Size: 175 827 186 48 24
Fragments arranged by size:
827 186 175 48 24
BspEI T'CCGG_A
Cuts at: 0 773 1260
Size: 773 487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 226 256 1260
Size: 226 30 1004
Fragments arranged by size:
1004 226 30
BsrDI GCAATG_nn'
Cuts at: 0 1085 1109 1260
Size: 1085 24 151
Fragments arranged by size:
1085 151 24
BstYI r'GATC_y
Cuts at: 0 561 770 910 1260
Size: 561 209 140 350
Fragments arranged by size:
561 350 209 140
BtsI GCAGTG_nn'
Cuts at: 0 808 1260
Size: 808 452
DraI TTT'AAA
Cuts at: 0 309 1260
Size: 309 951
EaeI y'GGCC_r
Cuts at: 0 394 882 1260
Size: 394 488 378
Fragments arranged by size:
488 394 378
EarI CTCTTCn'nnn_
Cuts at: 0 1007 1260
Size: 1007 253
EcoICRI GAG'CTC
Cuts at: 0 1000 1234 1260
Size: 1000 234 26
Fragments arranged by size:
1000 234 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 224 1055 1172 1260
Size: 197 27 831 117 88
Fragments arranged by size:
831 197 117 88 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 30 983 1260
Size: 30 953 277
Fragments arranged by size:
953 277 30
EcoO109I rG'GnC_Cy
Cuts at: 0 1215 1260
Size: 1215 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1218 1250 1260
Size: 1218 32 10
Fragments arranged by size:
1218 32 10
HincII GTy'rAC
Cuts at: 0 336 1260
Size: 336 924
HindIII A'AGCT_T
Cuts at: 0 634 1260
Size: 634 626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 1260
Size: 44 1216
MslI CAynn'nnrTG
Cuts at: 0 787 1260
Size: 787 473
MspA1I CmG'CkG
Cuts at: 0 1075 1260
Size: 1075 185
NspI r_CATG'y
Cuts at: 0 80 189 321 408 438 1149 1260
Size: 80 109 132 87 30 711 111
Fragments arranged by size:
711 132 111 109 87 80 30
PciI A'CATG_T
Cuts at: 0 76 1145 1260
Size: 76 1069 115
Fragments arranged by size:
1069 115 76
PflMI CCAn_nnn'nTGG
Cuts at: 0 1135 1158 1260
Size: 1135 23 102
Fragments arranged by size:
1135 102 23
PfoI T'CCnGG_A
Cuts at: 0 984 1188 1260
Size: 984 204 72
Fragments arranged by size:
984 204 72
PpuMI rG'GwC_Cy
Cuts at: 0 1215 1260
Size: 1215 45
PstI C_TGCA'G
Cuts at: 0 223 840 1020 1260
Size: 223 617 180 240
Fragments arranged by size:
617 240 223 180
PvuII CAG'CTG
Cuts at: 0 1075 1260
Size: 1075 185
SacI G_AGCT'C
Cuts at: 0 1002 1236 1260
Size: 1002 234 24
Fragments arranged by size:
1002 234 24
SapI GCTCTTCn'nnn_
Cuts at: 0 1007 1260
Size: 1007 253
ScaI AGT'ACT
Cuts at: 0 346 1260
Size: 346 914
SfcI C'TryA_G
Cuts at: 0 219 557 623 836 953 1016 1260
Size: 219 338 66 213 117 63 244
Fragments arranged by size:
338 244 219 213 117 66 63
SphI G_CATG'C
Cuts at: 0 189 408 1260
Size: 189 219 852
Fragments arranged by size:
852 219 189
SspI AAT'ATT
Cuts at: 0 591 1260
Size: 591 669
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 492 1169 1260
Size: 492 677 91
Fragments arranged by size:
677 492 91
TatI w'GTAC_w
Cuts at: 0 344 1260
Size: 344 916
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 1133 1165 1260
Size: 478 32 623 32 95
Fragments arranged by size:
623 478 95 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 79 1260
Size: 79 1181
XmnI GAAnn'nnTTC
Cuts at: 0 980 1260
Size: 980 280
Enzymes that do cut:
AcuI AflIII AleI AlwNI BanI BanII BbsI BclI
Bme1580I BmrI BpmI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BstYI BtsI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I
HincII HindIII MmeI MslI MspA1I NspI PciI PflMI
PfoI PpuMI PstI PvuII SacI SapI ScaI SfcI
SphI SspI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI StuI StyI SwaI
Tth111I XbaI XhoI XmaI ZraI