Restriction map for
pF1KB8664
(Linear) (Six-base) MAPSORT of: KIBB8664.seq Check: 7861 from: 1 to: 1227
KIBB8664.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 0 162 1227
Size: 162 1065
AccI GT'mk_AC
Cuts at: 0 1064 1148 1227
Size: 1064 84 79
Fragments arranged by size:
1064 84 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 852 949 1227
Size: 852 97 278
Fragments arranged by size:
852 278 97
ApaI G_GGCC'C
Cuts at: 0 1186 1227
Size: 1186 41
ApoI r'AATT_y
Cuts at: 0 319 505 1227
Size: 319 186 722
Fragments arranged by size:
722 319 186
AvaI C'yCGr_G
Cuts at: 0 964 1227
Size: 964 263
BanI G'GyrC_C
Cuts at: 0 364 1227
Size: 364 863
BanII G_rGCy'C
Cuts at: 0 436 796 972 1133 1186 1227
Size: 436 360 176 161 53 41
Fragments arranged by size:
436 360 176 161 53 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 681 991 1227
Size: 681 310 236
Fragments arranged by size:
681 310 236
BbvCI CC'TCA_GC
Cuts at: 0 92 886 1227
Size: 92 794 341
Fragments arranged by size:
794 341 92
Bme1580I G_kGCm'C
Cuts at: 0 1186 1227
Size: 1186 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 1064 1227
Size: 1064 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1227
Size: 100 1127
Bpu10I CC'TnA_GC
Cuts at: 0 92 124 886 1227
Size: 92 32 762 341
Fragments arranged by size:
762 341 92 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1227
Size: 154 1073
BsaI GGTCTCn'nnnn_
Cuts at: 0 927 1227
Size: 927 300
BsaBI GATnn'nnATC
Cuts at: 0 811 1227
Size: 811 416
BsaHI Gr'CG_yC
Cuts at: 0 159 1227
Size: 159 1068
BsaWI w'CCGG_w
Cuts at: 0 1154 1227
Size: 1154 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1207 1227
Size: 1207 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 478 699 843 1227
Size: 19 459 221 144 384
Fragments arranged by size:
459 384 221 144 19
BseYI C'CCAG_C
Cuts at: 0 796 1016 1227
Size: 796 220 211
Fragments arranged by size:
796 220 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 1227
Size: 115 1112
BsiHKAI G_wGCw'C
Cuts at: 0 908 1227
Size: 908 319
BsmBI CGTCTCn'nnnn_
Cuts at: 0 241 1122 1227
Size: 241 881 105
Fragments arranged by size:
881 241 105
Bsp1286I G_dGCh'C
Cuts at: 0 436 796 908 972 1133 1186 1227
Size: 436 360 112 64 161 53 41
Fragments arranged by size:
436 360 161 112 64 53 41
BspHI T'CATG_A
Cuts at: 0 723 1227
Size: 723 504
BsrFI r'CCGG_y
Cuts at: 0 572 1227
Size: 572 655
BssSI C'ACGA_G
Cuts at: 0 543 1227
Size: 543 684
BstEII G'GTnAC_C
Cuts at: 0 102 1227
Size: 102 1125
BtgI C'CryG_G
Cuts at: 0 1186 1227
Size: 1186 41
BtsI GCAGTG_nn'
Cuts at: 0 30 39 830 1227
Size: 30 9 791 397
Fragments arranged by size:
791 397 30 9
DraIII CAC_nnn'GTG
Cuts at: 0 300 1227
Size: 300 927
EarI CTCTTCn'nnn_
Cuts at: 0 343 925 1227
Size: 343 582 302
Fragments arranged by size:
582 343 302
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 681 1227
Size: 681 546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 852 949 1227
Size: 100 752 97 278
Fragments arranged by size:
752 278 100 97
EcoRI G'AATT_C
Cuts at: 0 319 505 1227
Size: 319 186 722
Fragments arranged by size:
722 319 186
HincII GTy'rAC
Cuts at: 0 1125 1227
Size: 1125 102
MslI CAynn'nnrTG
Cuts at: 0 302 493 1227
Size: 302 191 734
Fragments arranged by size:
734 302 191
MspA1I CmG'CkG
Cuts at: 0 50 848 860 1227
Size: 50 798 12 367
Fragments arranged by size:
798 367 50 12
NspI r_CATG'y
Cuts at: 0 604 1147 1227
Size: 604 543 80
Fragments arranged by size:
604 543 80
PasI CC'CwG_GG
Cuts at: 0 458 1227
Size: 458 769
PfoI T'CCnGG_A
Cuts at: 0 509 1227
Size: 509 718
PshAI GACnn'nnGTC
Cuts at: 0 1195 1227
Size: 1195 32
PspOMI G'GGCC_C
Cuts at: 0 1182 1227
Size: 1182 45
PstI C_TGCA'G
Cuts at: 0 455 825 1083 1227
Size: 455 370 258 144
Fragments arranged by size:
455 370 258 144
PvuII CAG'CTG
Cuts at: 0 848 1227
Size: 848 379
RsrII CG'GwC_CG
Cuts at: 0 1157 1227
Size: 1157 70
SfcI C'TryA_G
Cuts at: 0 451 821 1061 1079 1227
Size: 451 370 240 18 148
Fragments arranged by size:
451 370 240 148 18
SmaI CCC'GGG
Cuts at: 0 966 1227
Size: 966 261
SmlI C'TyrA_G
Cuts at: 0 169 1227
Size: 169 1058
StuI AGG'CCT
Cuts at: 0 394 1227
Size: 394 833
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 632 1227
Size: 632 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1207 1227
Size: 1207 20
XmaI C'CCGG_G
Cuts at: 0 964 1227
Size: 964 263
XmnI GAAnn'nnTTC
Cuts at: 0 988 1227
Size: 988 239
ZraI GAC'GTC
Cuts at: 0 160 1227
Size: 160 1067
Enzymes that do cut:
AatII AccI AcuI ApaI ApoI AvaI BanI BanII
BbsI BbvCI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BsrFI BssSI BstEII BtgI BtsI
DraIII EarI EciI Eco57MI EcoRI HincII MslI MspA1I
NspI PasI PfoI PshAI PspOMI PstI PvuII RsrII
SfcI SmaI SmlI StuI TaqII TstI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BsaAI BsiEI
BsiWI BsmI BspEI BspMI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EaeI EagI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PsiI
PspXI PsrI PvuI SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI Tth111I
XbaI XcmI XhoI