Restriction map for
pF1KB8665
(Linear) (Six-base) MAPSORT of: KIBB8665.seq Check: 4447 from: 1 to: 1497
KIBB8665.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1005 1497
Size: 1005 492
AccI GT'mk_AC
Cuts at: 0 790 1497
Size: 790 707
Acc65I G'GTAC_C
Cuts at: 0 1073 1497
Size: 1073 424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 979 1497
Size: 215 764 518
Fragments arranged by size:
764 518 215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 763 1497
Size: 763 734
AlwNI CAG_nnn'CTG
Cuts at: 0 534 846 1377 1497
Size: 534 312 531 120
Fragments arranged by size:
534 531 312 120
ApaI G_GGCC'C
Cuts at: 0 762 1497
Size: 762 735
ApaLI G'TGCA_C
Cuts at: 0 894 1497
Size: 894 603
ApoI r'AATT_y
Cuts at: 0 979 1497
Size: 979 518
AvaI C'yCGr_G
Cuts at: 0 754 1335 1497
Size: 754 581 162
Fragments arranged by size:
754 581 162
BanI G'GyrC_C
Cuts at: 0 729 1073 1497
Size: 729 344 424
Fragments arranged by size:
729 424 344
BanII G_rGCy'C
Cuts at: 0 20 762 1497
Size: 20 742 735
Fragments arranged by size:
742 735 20
BbvCI CC'TCA_GC
Cuts at: 0 1418 1497
Size: 1418 79
BglI GCCn_nnn'nGGC
Cuts at: 0 1196 1249 1497
Size: 1196 53 248
Fragments arranged by size:
1196 248 53
Bme1580I G_kGCm'C
Cuts at: 0 734 762 898 1342 1497
Size: 734 28 136 444 155
Fragments arranged by size:
734 444 155 136 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 571 580 604 1332 1354 1399 1425
Size: 394 177 9 24 728 22 45 26
Cuts at: 1425 1497
Size: 72
Fragments arranged by size:
728 394 177 72 45 26 24 22
9
Bpu10I CC'TnA_GC
Cuts at: 0 1418 1497
Size: 1418 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1444 1497
Size: 1444 53
BsaHI Gr'CG_yC
Cuts at: 0 1157 1497
Size: 1157 340
BsaWI w'CCGG_w
Cuts at: 0 852 1112 1497
Size: 852 260 385
Fragments arranged by size:
852 385 260
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 97 124 217 556 559 577 1165
Size: 64 33 27 93 339 3 18 588
Cuts at: 1165 1323 1497
Size: 158 174
Fragments arranged by size:
588 339 174 158 93 64 33 27
18 3
BseYI C'CCAG_C
Cuts at: 0 423 761 1095 1497
Size: 423 338 334 402
Fragments arranged by size:
423 402 338 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 859 1497
Size: 124 735 638
Fragments arranged by size:
735 638 124
BsiEI CG_ry'CG
Cuts at: 0 95 683 1068 1329 1497
Size: 95 588 385 261 168
Fragments arranged by size:
588 385 261 168 95
BsiHKAI G_wGCw'C
Cuts at: 0 20 898 934 1497
Size: 20 878 36 563
Fragments arranged by size:
878 563 36 20
Bsp1286I G_dGCh'C
Cuts at: 0 20 734 762 898 934 1342 1497
Size: 20 714 28 136 36 408 155
Fragments arranged by size:
714 408 155 136 36 28 20
BspEI T'CCGG_A
Cuts at: 0 1112 1497
Size: 1112 385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 1005 1061 1497
Size: 470 535 56 436
Fragments arranged by size:
535 470 436 56
BsrBI CCG'CTC
Cuts at: 0 555 1092 1331 1497
Size: 555 537 239 166
Fragments arranged by size:
555 537 239 166
BsrFI r'CCGG_y
Cuts at: 0 703 811 1497
Size: 703 108 686
Fragments arranged by size:
703 686 108
BssSI C'ACGA_G
Cuts at: 0 925 1497
Size: 925 572
BstAPI GCAn_nnn'nTGC
Cuts at: 0 846 1497
Size: 846 651
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 492 693 1497
Size: 396 96 201 804
Fragments arranged by size:
804 396 201 96
BstYI r'GATC_y
Cuts at: 0 123 470 855 1109 1256 1497
Size: 123 347 385 254 147 241
Fragments arranged by size:
385 347 254 241 147 123
BtgI C'CryG_G
Cuts at: 0 299 677 1497
Size: 299 378 820
Fragments arranged by size:
820 378 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 135 627 1497
Size: 135 492 870
Fragments arranged by size:
870 492 135
BtsI GCAGTG_nn'
Cuts at: 0 1215 1497
Size: 1215 282
EaeI y'GGCC_r
Cuts at: 0 680 701 884 1065 1497
Size: 680 21 183 181 432
Fragments arranged by size:
680 432 183 181 21
EagI C'GGCC_G
Cuts at: 0 680 1065 1497
Size: 680 385 432
Fragments arranged by size:
680 432 385
EarI CTCTTCn'nnn_
Cuts at: 0 29 41 74 1471 1497
Size: 29 12 33 1397 26
Fragments arranged by size:
1397 33 29 26 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 879 1497
Size: 879 618
EcoICRI GAG'CTC
Cuts at: 0 18 1497
Size: 18 1479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 394 571 580 604 979 1332 1354
Size: 215 179 177 9 24 375 353 22
Cuts at: 1354 1399 1425 1497
Size: 45 26 72
Fragments arranged by size:
375 353 215 179 177 72 45 26
24 22 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 724 1497
Size: 724 773
EcoO109I rG'GnC_Cy
Cuts at: 0 758 1170 1374 1497
Size: 758 412 204 123
Fragments arranged by size:
758 412 204 123
HaeII r_GCGC'y
Cuts at: 0 583 1497
Size: 583 914
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 1318 1350 1497
Size: 337 32 949 32 147
Fragments arranged by size:
949 337 147 32 32
HindIII A'AGCT_T
Cuts at: 0 227 1497
Size: 227 1270
KpnI G_GTAC'C
Cuts at: 0 1077 1497
Size: 1077 420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 1497
Size: 518 979
MslI CAynn'nnrTG
Cuts at: 0 490 697 1497
Size: 490 207 800
Fragments arranged by size:
800 490 207
MspA1I CmG'CkG
Cuts at: 0 120 534 1026 1050 1275 1497
Size: 120 414 492 24 225 222
Fragments arranged by size:
492 414 225 222 120 24
NaeI GCC'GGC
Cuts at: 0 705 1497
Size: 705 792
NgoMIV G'CCGG_C
Cuts at: 0 703 1497
Size: 703 794
NotI GC'GGCC_GC
Cuts at: 0 1065 1497
Size: 1065 432
PasI CC'CwG_GG
Cuts at: 0 522 1497
Size: 522 975
PflMI CCAn_nnn'nTGG
Cuts at: 0 161 1377 1497
Size: 161 1216 120
Fragments arranged by size:
1216 161 120
PfoI T'CCnGG_A
Cuts at: 0 341 628 1497
Size: 341 287 869
Fragments arranged by size:
869 341 287
PpuMI rG'GwC_Cy
Cuts at: 0 1170 1497
Size: 1170 327
PspOMI G'GGCC_C
Cuts at: 0 758 1497
Size: 758 739
PstI C_TGCA'G
Cuts at: 0 467 1274 1286 1497
Size: 467 807 12 211
Fragments arranged by size:
807 467 211 12
PvuI CG_AT'CG
Cuts at: 0 95 1497
Size: 95 1402
PvuII CAG'CTG
Cuts at: 0 120 534 1497
Size: 120 414 963
Fragments arranged by size:
963 414 120
SacI G_AGCT'C
Cuts at: 0 20 1497
Size: 20 1477
ScaI AGT'ACT
Cuts at: 0 352 1497
Size: 352 1145
SfcI C'TryA_G
Cuts at: 0 463 1270 1282 1497
Size: 463 807 12 215
Fragments arranged by size:
807 463 215 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1249 1497
Size: 1249 248
SmlI C'TyrA_G
Cuts at: 0 1459 1497
Size: 1459 38
StuI AGG'CCT
Cuts at: 0 568 1497
Size: 568 929
StyI C'CwwG_G
Cuts at: 0 380 725 1247 1497
Size: 380 345 522 250
Fragments arranged by size:
522 380 345 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 663 685 1497
Size: 535 128 22 812
Fragments arranged by size:
812 535 128 22
TatI w'GTAC_w
Cuts at: 0 350 1497
Size: 350 1147
Tth111I GACn'n_nGTC
Cuts at: 0 774 1497
Size: 774 723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 897 1497
Size: 897 600
XmnI GAAnn'nnTTC
Cuts at: 0 1435 1497
Size: 1435 62
Enzymes that do cut:
AarI AccI Acc65I AcuI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbvCI BglI Bme1580I BpmI
Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
KpnI MmeI MslI MspA1I NaeI NgoMIV NotI PasI
PflMI PfoI PpuMI PspOMI PstI PvuI PvuII SacI
ScaI SfcI SfiI SmlI StuI StyI TaqII TatI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI BsaI BsaAI BsaBI BsaXI BsiWI
BsmI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
MfeI MluI MscI NarI NcoI NdeI NheI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI RsrII SacII SalI SanDI SapI
SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI XbaI XhoI XmaI
ZraI