Restriction map for
pF1KB8666
(Linear) (Six-base) MAPSORT of: KIBB8666.seq Check: 3833 from: 1 to: 1086
KIBB8666.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 769 1086
Size: 442 327 317
Fragments arranged by size:
442 327 317
AfeI AGC'GCT
Cuts at: 0 346 1086
Size: 346 740
AflIII A'CryG_T
Cuts at: 0 969 1086
Size: 969 117
AlwNI CAG_nnn'CTG
Cuts at: 0 420 855 1086
Size: 420 435 231
Fragments arranged by size:
435 420 231
ApaI G_GGCC'C
Cuts at: 0 546 1086
Size: 546 540
ApoI r'AATT_y
Cuts at: 0 397 667 1086
Size: 397 270 419
Fragments arranged by size:
419 397 270
AvaI C'yCGr_G
Cuts at: 0 524 1086
Size: 524 562
BanII G_rGCy'C
Cuts at: 0 546 740 1086
Size: 546 194 346
Fragments arranged by size:
546 346 194
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 448 1086
Size: 227 221 638
Fragments arranged by size:
638 227 221
Bme1580I G_kGCm'C
Cuts at: 0 211 546 1086
Size: 211 335 540
Fragments arranged by size:
540 335 211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1026 1086
Size: 1026 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1086
Size: 609 477
BsaI GGTCTCn'nnnn_
Cuts at: 0 859 1086
Size: 859 227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 582 612 624 654 862 892 1086
Size: 582 30 12 30 208 30 194
Fragments arranged by size:
582 208 194 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1018 1086
Size: 1018 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 947 1086
Size: 868 79 139
Fragments arranged by size:
868 139 79
BsiHKAI G_wGCw'C
Cuts at: 0 715 740 880 1086
Size: 715 25 140 206
Fragments arranged by size:
715 206 140 25
Bsp1286I G_dGCh'C
Cuts at: 0 211 546 715 740 880 1086
Size: 211 335 169 25 140 206
Fragments arranged by size:
335 211 206 169 140 25
BsrBI CCG'CTC
Cuts at: 0 1055 1086
Size: 1055 31
BssSI C'ACGA_G
Cuts at: 0 871 1086
Size: 871 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 49 710 855 1086
Size: 49 661 145 231
Fragments arranged by size:
661 231 145 49
BstBI TT'CG_AA
Cuts at: 0 93 1086
Size: 93 993
BstXI CCAn_nnnn'nTGG
Cuts at: 0 888 948 1086
Size: 888 60 138
Fragments arranged by size:
888 138 60
Bsu36I CC'TnA_GG
Cuts at: 0 413 625 1086
Size: 413 212 461
Fragments arranged by size:
461 413 212
BtgI C'CryG_G
Cuts at: 0 1013 1086
Size: 1013 73
ClaI AT'CG_AT
Cuts at: 0 752 1086
Size: 752 334
EaeI y'GGCC_r
Cuts at: 0 764 807 1086
Size: 764 43 279
Fragments arranged by size:
764 279 43
EarI CTCTTCn'nnn_
Cuts at: 0 728 818 1086
Size: 728 90 268
Fragments arranged by size:
728 268 90
EcoICRI GAG'CTC
Cuts at: 0 738 1086
Size: 738 348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 609 769 1086
Size: 442 167 160 317
Fragments arranged by size:
442 317 167 160
EcoO109I rG'GnC_Cy
Cuts at: 0 542 1086
Size: 542 544
EcoRI G'AATT_C
Cuts at: 0 397 1086
Size: 397 689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 472 1086
Size: 472 614
HaeII r_GCGC'y
Cuts at: 0 348 1086
Size: 348 738
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 1086
Size: 133 32 921
Fragments arranged by size:
921 133 32
HincII GTy'rAC
Cuts at: 0 266 1086
Size: 266 820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1086
Size: 773 313
MscI TGG'CCA
Cuts at: 0 766 809 1086
Size: 766 43 277
Fragments arranged by size:
766 277 43
MspA1I CmG'CkG
Cuts at: 0 904 1086
Size: 904 182
NspI r_CATG'y
Cuts at: 0 455 973 1086
Size: 455 518 113
Fragments arranged by size:
518 455 113
PciI A'CATG_T
Cuts at: 0 969 1086
Size: 969 117
PspOMI G'GGCC_C
Cuts at: 0 542 1086
Size: 542 544
SacI G_AGCT'C
Cuts at: 0 740 1086
Size: 740 346
SapI GCTCTTCn'nnn_
Cuts at: 0 728 1086
Size: 728 358
SfcI C'TryA_G
Cuts at: 0 501 1086
Size: 501 585
StuI AGG'CCT
Cuts at: 0 1026 1086
Size: 1026 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 913 1086
Size: 913 173
TatI w'GTAC_w
Cuts at: 0 129 1086
Size: 129 957
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 582 614 862 894 959 991 1086
Size: 582 32 248 32 65 32 95
Fragments arranged by size:
582 248 95 65 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 99 239 1086
Size: 99 140 847
Fragments arranged by size:
847 140 99
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApaI ApoI AvaI BanII
BbsI Bme1580I BplI BpmI BsaI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BsrBI BssSI BstAPI BstBI BstXI Bsu36I
BtgI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII MmeI MscI MspA1I NspI
PciI PspOMI SacI SapI SfcI StuI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BstEII BstYI BstZ17I BtgZI BtsI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI