(Linear) (Six-base) MAPSORT of: KIBB8667.seq  Check: 2329  from: 1  to: 1272



KIBB8667.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AccI GT'mk_AC

Cuts at:      0     437    1272 
   Size:        437     835
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     667    1272 
   Size:        667     605
 
AlwNI CAG_nnn'CTG

Cuts at:      0     183    1272 
   Size:        183    1089
 
ApaI G_GGCC'C

Cuts at:      0    1228    1272 
   Size:       1228      44
 
ApoI r'AATT_y

Cuts at:      0     421     767    1272 
   Size:        421     346     505

  Fragments arranged by size: 

                 505     421     346
 
BamHI G'GATC_C

Cuts at:      0     360     724    1272 
   Size:        360     364     548

  Fragments arranged by size: 

                 548     364     360
 
BanII G_rGCy'C

Cuts at:      0     200    1228    1272 
   Size:        200    1028      44

  Fragments arranged by size: 

                1028     200      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     862    1272 
   Size:        862     410
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1042    1076    1272 
   Size:       1042      34     196

  Fragments arranged by size: 

                1042     196      34
 
BfrBI ATG'CAT

Cuts at:      0     556    1272 
   Size:        556     716
 
Bme1580I G_kGCm'C

Cuts at:      0     154    1228    1272 
   Size:        154    1074      44

  Fragments arranged by size: 

                1074     154      44
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      50    1272 
   Size:         50    1222
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     899    1272 
   Size:        899     373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1142    1272 
   Size:       1142     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1170    1272 
   Size:       1170     102
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     105     274    1272 
   Size:        105     169     998

  Fragments arranged by size: 

                 998     169     105
 
BseYI C'CCAG_C

Cuts at:      0     201    1221    1272 
   Size:        201    1020      51

  Fragments arranged by size: 

                1020     201      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     833    1272 
   Size:        833     439
 
BsiEI CG_ry'CG

Cuts at:      0     160    1272 
   Size:        160    1112
 
BsmI GAATG_Cn'

Cuts at:      0     572    1272 
   Size:        572     700
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     791    1272 
   Size:        791     481
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     200    1228    1272 
   Size:        154      46    1028      44

  Fragments arranged by size: 

                1028     154      46      44
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      25    1272 
   Size:         25    1247
 
BsrBI CCG'CTC

Cuts at:      0    1184    1272 
   Size:       1184      88
 
BsrDI GCAATG_nn'

Cuts at:      0     143    1272 
   Size:        143    1129
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1221    1272 
   Size:       1221      51
 
BstYI r'GATC_y

Cuts at:      0     360     724    1197    1272 
   Size:        360     364     473      75

  Fragments arranged by size: 

                 473     364     360      75
 
BstZ17I GTA'TAC

Cuts at:      0     438    1272 
   Size:        438     834
 
BtgI C'CryG_G

Cuts at:      0     864    1272 
   Size:        864     408
 
BtsI GCAGTG_nn'

Cuts at:      0      14      56    1272 
   Size:         14      42    1216

  Fragments arranged by size: 

                1216      42      14
 
DraI TTT'AAA

Cuts at:      0    1113    1272 
   Size:       1113     159
 
EaeI y'GGCC_r

Cuts at:      0    1244    1272 
   Size:       1244      28
 
EarI CTCTTCn'nnn_

Cuts at:      0     298    1272 
   Size:        298     974
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1200    1272 
   Size:       1200      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     667    1142    1272 
   Size:        667     475     130

  Fragments arranged by size: 

                 667     475     130
 
EcoO109I rG'GnC_Cy

Cuts at:      0      97    1272 
   Size:         97    1175
 
EcoRI G'AATT_C

Cuts at:      0     767    1272 
   Size:        767     505
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      81     113     610     642    1272 
   Size:         81      32     497      32     630

  Fragments arranged by size: 

                 630     497      81      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     219    1272 
   Size:        219    1053
 
MslI CAynn'nnrTG

Cuts at:      0     548     789     904    1272 
   Size:        548     241     115     368

  Fragments arranged by size: 

                 548     368     241     115
 
MspA1I CmG'CkG

Cuts at:      0     125     180    1272 
   Size:        125      55    1092

  Fragments arranged by size: 

                1092     125      55
 
NsiI A_TGCA'T

Cuts at:      0     558    1272 
   Size:        558     714
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1234    1272 
   Size:       1234      38
 
PpuMI rG'GwC_Cy

Cuts at:      0      97    1272 
   Size:         97    1175
 
PshAI GACnn'nnGTC

Cuts at:      0     138    1272 
   Size:        138    1134
 
PspOMI G'GGCC_C

Cuts at:      0    1224    1272 
   Size:       1224      48
 
PstI C_TGCA'G

Cuts at:      0      51    1272 
   Size:         51    1221
 
PvuII CAG'CTG

Cuts at:      0     180    1272 
   Size:        180    1092
 
SapI GCTCTTCn'nnn_

Cuts at:      0     298    1272 
   Size:        298     974
 
ScaI AGT'ACT

Cuts at:      0      39    1272 
   Size:         39    1233
 
SfcI C'TryA_G

Cuts at:      0      47    1272 
   Size:         47    1225
 
SmlI C'TyrA_G

Cuts at:      0    1185    1272 
   Size:       1185      87
 
SspI AAT'ATT

Cuts at:      0     276     465    1272 
   Size:        276     189     807

  Fragments arranged by size: 

                 807     276     189
 
TatI w'GTAC_w

Cuts at:      0      37    1272 
   Size:         37    1235
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     905     937    1272 
   Size:        905      32     335

  Fragments arranged by size: 

                 905     335      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     220    1272 
   Size:        220    1052
 
XmnI GAAnn'nnTTC

Cuts at:      0    1129    1272 
   Size:       1129     143
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApaI     ApoI    BamHI    BanII     BbsI
     BcgI    BfrBI Bme1580I     BmrI     BplI     BpmI    BpuEI    BseRI
    BseYI     BsgI    BsiEI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BstXI    BstYI  BstZ17I     BtgI     BtsI     DraI     EaeI
     EarI     EciI  Eco57MI EcoO109I    EcoRI    Hin4I     MmeI     MslI
   MspA1I     NsiI    PflMI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SapI     ScaI     SfcI     SmlI     SspI     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI    BmgBI     BmtI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI  BsiHKAI    BsiWI    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     ZraI