(Linear) (Six-base) MAPSORT of: KIBB8668.seq  Check: 7425  from: 1  to: 1389



KIBB8668.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AccI GT'mk_AC

Cuts at:      0    1054    1389 
   Size:       1054     335
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395    1389 
   Size:        395     994
 
AflIII A'CryG_T

Cuts at:      0     205    1389 
   Size:        205    1184
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     785     817    1389 
   Size:        785      32     572

  Fragments arranged by size: 

                 785     572      32
 
ApaLI G'TGCA_C

Cuts at:      0     708    1389 
   Size:        708     681
 
ApoI r'AATT_y

Cuts at:      0     388    1389 
   Size:        388    1001
 
AvaI C'yCGr_G

Cuts at:      0     133     629    1389 
   Size:        133     496     760

  Fragments arranged by size: 

                 760     496     133
 
BanII G_rGCy'C

Cuts at:      0     251    1389 
   Size:        251    1138
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     369    1389 
   Size:        369    1020
 
Bme1580I G_kGCm'C

Cuts at:      0     712    1389 
   Size:        712     677
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     108    1389 
   Size:        108    1281
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     172    1181    1389 
   Size:        113      59    1009     208

  Fragments arranged by size: 

                1009     208     113      59
 
Bpu10I CC'TnA_GC

Cuts at:      0     237    1389 
   Size:        237    1152
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     890    1389 
   Size:        890     499
 
BsaAI yAC'GTr

Cuts at:      0     893    1389 
   Size:        893     496
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     785     815    1389 
   Size:        785      30     574

  Fragments arranged by size: 

                 785     574      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28      58    1389 
   Size:         28      30    1331

  Fragments arranged by size: 

                1331      30      28
 
BseYI C'CCAG_C

Cuts at:      0    1092    1389 
   Size:       1092     297
 
BsiHKAI G_wGCw'C

Cuts at:      0     178     251     712    1389 
   Size:        178      73     461     677

  Fragments arranged by size: 

                 677     461     178      73
 
BsmI GAATG_Cn'

Cuts at:      0     670    1389 
   Size:        670     719
 
Bsp1286I G_dGCh'C

Cuts at:      0     178     251     712    1389 
   Size:        178      73     461     677

  Fragments arranged by size: 

                 677     461     178      73
 
BsrDI GCAATG_nn'

Cuts at:      0     423     713     956    1389 
   Size:        423     290     243     433

  Fragments arranged by size: 

                 433     423     290     243
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     958    1389 
   Size:        958     431
 
BstBI TT'CG_AA

Cuts at:      0     167    1389 
   Size:        167    1222
 
BstEII G'GTnAC_C

Cuts at:      0    1251    1389 
   Size:       1251     138
 
BstYI r'GATC_y

Cuts at:      0     570    1389 
   Size:        570     819
 
BstZ17I GTA'TAC

Cuts at:      0    1055    1389 
   Size:       1055     334
 
Bsu36I CC'TnA_GG

Cuts at:      0     368     776    1389 
   Size:        368     408     613

  Fragments arranged by size: 

                 613     408     368
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     492    1389 
   Size:        492     897
 
BtsI GCAGTG_nn'

Cuts at:      0     775    1389 
   Size:        775     614
 
DraI TTT'AAA

Cuts at:      0     618     987    1389 
   Size:        618     369     402

  Fragments arranged by size: 

                 618     402     369
 
EarI CTCTTCn'nnn_

Cuts at:      0     548    1389 
   Size:        548     841
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1389 
   Size:         19    1370
 
EcoICRI GAG'CTC

Cuts at:      0     249    1389 
   Size:        249    1140
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     172     395    1181    1389 
   Size:        113      59     223     786     208

  Fragments arranged by size: 

                 786     223     208     113      59
 
EcoRV GAT'ATC

Cuts at:      0     539    1389 
   Size:        539     850
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     233     265     562     594     850     882    1389 
   Size:        233      32     297      32     256      32     507

  Fragments arranged by size: 

                 507     297     256     233      32      32      32
 
MspA1I CmG'CkG

Cuts at:      0     964    1389 
   Size:        964     425
 
NspI r_CATG'y

Cuts at:      0     209     534    1389 
   Size:        209     325     855

  Fragments arranged by size: 

                 855     325     209
 
PciI A'CATG_T

Cuts at:      0     205    1389 
   Size:        205    1184
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     518     811    1389 
   Size:        518     293     578

  Fragments arranged by size: 

                 578     518     293
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     785     817    1389 
   Size:        785      32     572

  Fragments arranged by size: 

                 785     572      32
 
PspXI vC'TCGA_Gb

Cuts at:      0     133    1389 
   Size:        133    1256
 
PstI C_TGCA'G

Cuts at:      0     387     936    1389 
   Size:        387     549     453

  Fragments arranged by size: 

                 549     453     387
 
PvuII CAG'CTG

Cuts at:      0     964    1389 
   Size:        964     425
 
SacI G_AGCT'C

Cuts at:      0     251    1389 
   Size:        251    1138
 
SfcI C'TryA_G

Cuts at:      0     308     383     932    1144    1389 
   Size:        308      75     549     212     245

  Fragments arranged by size: 

                 549     308     245     212      75
 
SmaI CCC'GGG

Cuts at:      0     631    1389 
   Size:        631     758
 
SmlI C'TyrA_G

Cuts at:      0     133     905    1389 
   Size:        133     772     484

  Fragments arranged by size: 

                 772     484     133
 
SnaBI TAC'GTA

Cuts at:      0     893    1389 
   Size:        893     496
 
SphI G_CATG'C

Cuts at:      0     534    1389 
   Size:        534     855
 
SspI AAT'ATT

Cuts at:      0     879    1119    1389 
   Size:        879     240     270

  Fragments arranged by size: 

                 879     270     240
 
StuI AGG'CCT

Cuts at:      0     348    1389 
   Size:        348    1041
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      68    1389 
   Size:         68    1321
 
TatI w'GTAC_w

Cuts at:      0    1362    1389 
   Size:       1362      27
 
XhoI C'TCGA_G

Cuts at:      0     133    1389 
   Size:        133    1256
 
XmaI C'CCGG_G

Cuts at:      0     629    1389 
   Size:        629     760
 
XmnI GAAnn'nnTTC

Cuts at:      0     800    1389 
   Size:        800     589
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AloI    ApaLI     ApoI     AvaI    BanII
     BbsI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     DraI     EarI
     EciI  EcoICRI  Eco57MI    EcoRV    Hin4I   MspA1I     NspI     PciI
    PflMI     PpiI    PspXI     PstI    PvuII     SacI     SfcI     SmaI
     SmlI    SnaBI     SphI     SspI     StuI    TaqII     TatI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BsaI
    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstXI
     BtgI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SpeI     SrfI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     ZraI