Restriction map for
pF1KB8673
(Linear) (Six-base) MAPSORT of: KIBB8673.seq Check: 719 from: 1 to: 1971
KIBB8673.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1167 1316 1971
Size: 404 763 149 655
Fragments arranged by size:
763 655 404 149
AflIII A'CryG_T
Cuts at: 0 953 1971
Size: 953 1018
ApoI r'AATT_y
Cuts at: 0 722 925 970 1042 1472 1971
Size: 722 203 45 72 430 499
Fragments arranged by size:
722 499 430 203 72 45
AvaI C'yCGr_G
Cuts at: 0 1081 1118 1646 1971
Size: 1081 37 528 325
Fragments arranged by size:
1081 528 325 37
BanII G_rGCy'C
Cuts at: 0 629 1971
Size: 629 1342
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 1971
Size: 115 1856
BciVI GTATCCnnnnn_n'
Cuts at: 0 1088 1971
Size: 1088 883
BfrBI ATG'CAT
Cuts at: 0 1368 1971
Size: 1368 603
BglII A'GATC_T
Cuts at: 0 220 1971
Size: 220 1751
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 1971
Size: 490 1481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1971
Size: 374 1597
Bpu10I CC'TnA_GC
Cuts at: 0 23 1971
Size: 23 1948
BsaI GGTCTCn'nnnn_
Cuts at: 0 1197 1971
Size: 1197 774
BsaWI w'CCGG_w
Cuts at: 0 363 1971
Size: 363 1608
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 372 402 1257 1287 1971
Size: 372 30 855 30 684
Fragments arranged by size:
855 684 372 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 44 1265 1971
Size: 44 1221 706
Fragments arranged by size:
1221 706 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1971
Size: 61 1910
BsiHKAI G_wGCw'C
Cuts at: 0 629 863 1971
Size: 629 234 1108
Fragments arranged by size:
1108 629 234
BsmI GAATG_Cn'
Cuts at: 0 948 1971
Size: 948 1023
Bsp1286I G_dGCh'C
Cuts at: 0 629 863 1971
Size: 629 234 1108
Fragments arranged by size:
1108 629 234
BsrBI CCG'CTC
Cuts at: 0 1217 1971
Size: 1217 754
BsrDI GCAATG_nn'
Cuts at: 0 1017 1971
Size: 1017 954
BssSI C'ACGA_G
Cuts at: 0 664 1971
Size: 664 1307
BstXI CCAn_nnnn'nTGG
Cuts at: 0 800 1971
Size: 800 1171
BstYI r'GATC_y
Cuts at: 0 220 1971
Size: 220 1751
Bsu36I CC'TnA_GG
Cuts at: 0 157 1971
Size: 157 1814
BtgI C'CryG_G
Cuts at: 0 278 1971
Size: 278 1693
BtsI GCAGTG_nn'
Cuts at: 0 1842 1971
Size: 1842 129
DraI TTT'AAA
Cuts at: 0 1464 1971
Size: 1464 507
DraIII CAC_nnn'GTG
Cuts at: 0 1255 1971
Size: 1255 716
DrdI GACnn_nn'nnGTC
Cuts at: 0 1648 1971
Size: 1648 323
EaeI y'GGCC_r
Cuts at: 0 1557 1763 1971
Size: 1557 206 208
Fragments arranged by size:
1557 208 206
EarI CTCTTCn'nnn_
Cuts at: 0 191 641 1216 1971
Size: 191 450 575 755
Fragments arranged by size:
755 575 450 191
EcoICRI GAG'CTC
Cuts at: 0 627 1971
Size: 627 1344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 404 1167 1316 1971
Size: 374 30 763 149 655
Fragments arranged by size:
763 655 374 149 30
EcoO109I rG'GnC_Cy
Cuts at: 0 1814 1971
Size: 1814 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 807 839 1062 1094 1971
Size: 807 32 223 32 877
Fragments arranged by size:
877 807 223 32 32
HincII GTy'rAC
Cuts at: 0 1017 1971
Size: 1017 954
MscI TGG'CCA
Cuts at: 0 1765 1971
Size: 1765 206
MslI CAynn'nnrTG
Cuts at: 0 952 1971
Size: 952 1019
NcoI C'CATG_G
Cuts at: 0 278 1971
Size: 278 1693
NsiI A_TGCA'T
Cuts at: 0 1370 1971
Size: 1370 601
NspI r_CATG'y
Cuts at: 0 1666 1971
Size: 1666 305
PflMI CCAn_nnn'nTGG
Cuts at: 0 1611 1971
Size: 1611 360
PspXI vC'TCGA_Gb
Cuts at: 0 1646 1971
Size: 1646 325
PstI C_TGCA'G
Cuts at: 0 1407 1971
Size: 1407 564
SacI G_AGCT'C
Cuts at: 0 629 1971
Size: 629 1342
ScaI AGT'ACT
Cuts at: 0 621 1971
Size: 621 1350
SfcI C'TryA_G
Cuts at: 0 1050 1403 1971
Size: 1050 353 568
Fragments arranged by size:
1050 568 353
SmlI C'TyrA_G
Cuts at: 0 1646 1971
Size: 1646 325
SpeI A'CTAG_T
Cuts at: 0 517 1971
Size: 517 1454
SphI G_CATG'C
Cuts at: 0 1666 1971
Size: 1666 305
SspI AAT'ATT
Cuts at: 0 176 1971
Size: 176 1795
StyI C'CwwG_G
Cuts at: 0 278 1971
Size: 278 1693
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 509 1971
Size: 509 1462
TatI w'GTAC_w
Cuts at: 0 235 619 1971
Size: 235 384 1352
Fragments arranged by size:
1352 384 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 1257 1289 1971
Size: 372 32 853 32 682
Fragments arranged by size:
853 682 372 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 538 1971
Size: 538 1433
XbaI T'CTAG_A
Cuts at: 0 189 1971
Size: 189 1782
XhoI C'TCGA_G
Cuts at: 0 1646 1971
Size: 1646 325
XmnI GAAnn'nnTTC
Cuts at: 0 1045 1971
Size: 1045 926
Enzymes that do cut:
AcuI AflIII ApoI AvaI BanII BbsI BciVI BfrBI
BglII BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BssSI BstXI
BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I Hin4I HincII MscI MslI
NcoI NsiI NspI PflMI PspXI PstI SacI ScaI
SfcI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BglI BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI StuI SwaI XcmI XmaI ZraI