Restriction map for
pF1KB8675
(Linear) (Six-base) MAPSORT of: KIBB8675.seq Check: 3030 from: 1 to: 1866
KIBB8675.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 791 1866
Size: 282 509 1075
Fragments arranged by size:
1075 509 282
AatII G_ACGT'C
Cuts at: 0 476 694 1866
Size: 476 218 1172
Fragments arranged by size:
1172 476 218
AccI GT'mk_AC
Cuts at: 0 1328 1866
Size: 1328 538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1866
Size: 1150 716
AfeI AGC'GCT
Cuts at: 0 169 1866
Size: 169 1697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 1690 1866
Size: 196 1494 176
Fragments arranged by size:
1494 196 176
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 1866
Size: 484 32 1350
Fragments arranged by size:
1350 484 32
AlwNI CAG_nnn'CTG
Cuts at: 0 81 282 324 357 1866
Size: 81 201 42 33 1509
Fragments arranged by size:
1509 201 81 42 33
ApaI G_GGCC'C
Cuts at: 0 1079 1371 1866
Size: 1079 292 495
Fragments arranged by size:
1079 495 292
AvaI C'yCGr_G
Cuts at: 0 388 1072 1567 1866
Size: 388 684 495 299
Fragments arranged by size:
684 495 388 299
BanI G'GyrC_C
Cuts at: 0 58 1379 1540 1699 1866
Size: 58 1321 161 159 167
Fragments arranged by size:
1321 167 161 159 58
BanII G_rGCy'C
Cuts at: 0 526 1079 1371 1715 1866
Size: 526 553 292 344 151
Fragments arranged by size:
553 526 344 292 151
BbeI G_GCGC'C
Cuts at: 0 1383 1866
Size: 1383 483
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 683 694 1866
Size: 449 234 11 1172
Fragments arranged by size:
1172 449 234 11
BbvCI CC'TCA_GC
Cuts at: 0 1019 1866
Size: 1019 847
BglI GCCn_nnn'nGGC
Cuts at: 0 115 1866
Size: 115 1751
BglII A'GATC_T
Cuts at: 0 1055 1866
Size: 1055 811
BlpI GC'TnA_GC
Cuts at: 0 34 902 979 1866
Size: 34 868 77 887
Fragments arranged by size:
887 868 77 34
Bme1580I G_kGCm'C
Cuts at: 0 61 111 924 1050 1079 1371 1702 1866
Size: 61 50 813 126 29 292 331 164
Fragments arranged by size:
813 331 292 164 126 61 50 29
BmgBI CAC'GTC
Cuts at: 0 1575 1866
Size: 1575 291
BmrI ACTGGGnnnn_n'
Cuts at: 0 829 1866
Size: 829 1037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 174 1221 1866
Size: 174 1047 645
Fragments arranged by size:
1047 645 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 703 946 1072 1808 1809 1866
Size: 103 600 243 126 736 1 57
Fragments arranged by size:
736 600 243 126 103 57 1
Bpu10I CC'TnA_GC
Cuts at: 0 1019 1866
Size: 1019 847
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1638 1866
Size: 1638 228
BsaI GGTCTCn'nnnn_
Cuts at: 0 1150 1502 1804 1866
Size: 1150 352 302 62
Fragments arranged by size:
1150 352 302 62
BsaHI Gr'CG_yC
Cuts at: 0 300 446 473 691 1380 1866
Size: 300 146 27 218 689 486
Fragments arranged by size:
689 486 300 218 146 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 536 566 676 706 1147 1177
Size: 304 30 202 30 110 30 441 30
Cuts at: 1177 1866
Size: 689
Fragments arranged by size:
689 441 304 202 110 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 110 346 406 409 475 557 1036
Size: 40 70 236 60 3 66 82 479
Cuts at: 1036 1261 1866
Size: 225 605
Fragments arranged by size:
605 479 236 225 82 70 66 60
40 3
BseYI C'CCAG_C
Cuts at: 0 54 213 383 525 834 1534 1781 1866
Size: 54 159 170 142 309 700 247 85
Fragments arranged by size:
700 309 247 170 159 142 85 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 586 1866
Size: 328 258 1280
Fragments arranged by size:
1280 328 258
Bsp1286I G_dGCh'C
Cuts at: 0 61 111 526 924 1050 1079 1371 1702
Size: 61 50 415 398 126 29 292 331
Cuts at: 1702 1715 1866
Size: 13 151
Fragments arranged by size:
415 398 331 292 151 126 61 50
29 13
BspHI T'CATG_A
Cuts at: 0 416 1188 1866
Size: 416 772 678
Fragments arranged by size:
772 678 416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 282 791 1866
Size: 282 509 1075
Fragments arranged by size:
1075 509 282
BsrBI CCG'CTC
Cuts at: 0 1251 1866
Size: 1251 615
BsrFI r'CCGG_y
Cuts at: 0 139 1376 1866
Size: 139 1237 490
Fragments arranged by size:
1237 490 139
BsrGI T'GTAC_A
Cuts at: 0 1163 1866
Size: 1163 703
BstAPI GCAn_nnn'nTGC
Cuts at: 0 282 357 408 1866
Size: 282 75 51 1458
Fragments arranged by size:
1458 282 75 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1833 1866
Size: 1833 33
BstYI r'GATC_y
Cuts at: 0 425 578 929 1055 1172 1866
Size: 425 153 351 126 117 694
Fragments arranged by size:
694 425 351 153 126 117
Bsu36I CC'TnA_GG
Cuts at: 0 134 1657 1866
Size: 134 1523 209
Fragments arranged by size:
1523 209 134
BtgI C'CryG_G
Cuts at: 0 19 377 839 1832 1866
Size: 19 358 462 993 34
Fragments arranged by size:
993 462 358 34 19
BtsI GCAGTG_nn'
Cuts at: 0 372 1500 1614 1674 1866
Size: 372 1128 114 60 192
Fragments arranged by size:
1128 372 192 114 60
EaeI y'GGCC_r
Cuts at: 0 2 800 968 1094 1866
Size: 2 798 168 126 772
Fragments arranged by size:
798 772 168 126 2
EarI CTCTTCn'nnn_
Cuts at: 0 1766 1866
Size: 1766 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 703 946 1072 1150 1808 1809 1866
Size: 103 600 243 126 78 658 1 57
Fragments arranged by size:
658 600 243 126 103 78 57 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 536 1132 1866
Size: 536 596 734
Fragments arranged by size:
734 596 536
EcoO109I rG'GnC_Cy
Cuts at: 0 942 1367 1368 1579 1866
Size: 942 425 1 211 287
Fragments arranged by size:
942 425 287 211 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 423 1866
Size: 423 1443
FspI TGC'GCA
Cuts at: 0 1357 1866
Size: 1357 509
HaeII r_GCGC'y
Cuts at: 0 171 1275 1286 1383 1866
Size: 171 1104 11 97 483
Fragments arranged by size:
1104 483 171 97 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 1866
Size: 676 32 1158
Fragments arranged by size:
1158 676 32
HincII GTy'rAC
Cuts at: 0 1344 1866
Size: 1344 522
KasI G'GCGC_C
Cuts at: 0 1379 1866
Size: 1379 487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1866
Size: 416 1450
MscI TGG'CCA
Cuts at: 0 4 802 970 1096 1866
Size: 4 798 168 126 770
Fragments arranged by size:
798 770 168 126 4
MslI CAynn'nnrTG
Cuts at: 0 10 1234 1866
Size: 10 1224 632
Fragments arranged by size:
1224 632 10
MspA1I CmG'CkG
Cuts at: 0 21 1307 1588 1628 1866
Size: 21 1286 281 40 238
Fragments arranged by size:
1286 281 238 40 21
NaeI GCC'GGC
Cuts at: 0 141 1866
Size: 141 1725
NarI GG'CG_CC
Cuts at: 0 1380 1866
Size: 1380 486
NcoI C'CATG_G
Cuts at: 0 1832 1866
Size: 1832 34
NgoMIV G'CCGG_C
Cuts at: 0 139 1866
Size: 139 1727
PflMI CCAn_nnn'nTGG
Cuts at: 0 81 1866
Size: 81 1785
PfoI T'CCnGG_A
Cuts at: 0 806 932 1646 1866
Size: 806 126 714 220
Fragments arranged by size:
806 714 220 126
PpuMI rG'GwC_Cy
Cuts at: 0 942 1579 1866
Size: 942 637 287
Fragments arranged by size:
942 637 287
PspOMI G'GGCC_C
Cuts at: 0 1075 1367 1866
Size: 1075 292 499
Fragments arranged by size:
1075 499 292
PspXI vC'TCGA_Gb
Cuts at: 0 388 1567 1866
Size: 388 1179 299
Fragments arranged by size:
1179 388 299
PstI C_TGCA'G
Cuts at: 0 362 1694 1866
Size: 362 1332 172
Fragments arranged by size:
1332 362 172
PvuII CAG'CTG
Cuts at: 0 1307 1588 1866
Size: 1307 281 278
Fragments arranged by size:
1307 281 278
SacII CC_GC'GG
Cuts at: 0 22 1866
Size: 22 1844
SapI GCTCTTCn'nnn_
Cuts at: 0 1766 1866
Size: 1766 100
SexAI A'CCwGG_T
Cuts at: 0 608 1866
Size: 608 1258
SfcI C'TryA_G
Cuts at: 0 260 358 1583 1690 1866
Size: 260 98 1225 107 176
Fragments arranged by size:
1225 260 176 107 98
SfoI GGC'GCC
Cuts at: 0 1381 1866
Size: 1381 485
SmlI C'TyrA_G
Cuts at: 0 388 1567 1617 1866
Size: 388 1179 50 249
Fragments arranged by size:
1179 388 249 50
SspI AAT'ATT
Cuts at: 0 1033 1866
Size: 1033 833
StuI AGG'CCT
Cuts at: 0 1318 1866
Size: 1318 548
StyI C'CwwG_G
Cuts at: 0 62 1466 1832 1866
Size: 62 1404 366 34
Fragments arranged by size:
1404 366 62 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 848 1541 1866
Size: 848 693 325
Fragments arranged by size:
848 693 325
TatI w'GTAC_w
Cuts at: 0 764 890 1163 1866
Size: 764 126 273 703
Fragments arranged by size:
764 703 273 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 304 336 536 568 914 946 1866
Size: 304 32 200 32 346 32 920
Fragments arranged by size:
920 346 304 200 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 486 1110 1866
Size: 486 624 756
Fragments arranged by size:
756 624 486
XhoI C'TCGA_G
Cuts at: 0 388 1567 1866
Size: 388 1179 299
Fragments arranged by size:
1179 388 299
ZraI GAC'GTC
Cuts at: 0 474 692 1866
Size: 474 218 1174
Fragments arranged by size:
1174 474 218
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AlfI AloI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BbvCI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacII SapI SexAI
SfcI SfoI SmlI SspI StuI StyI TaqII TatI
TstI XcmI XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AleI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BmtI BsaAI BsaBI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BsrDI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XmaI XmnI