Restriction map for
pF1KB8676
(Linear) (Six-base) MAPSORT of: KIBB8676.seq Check: 3823 from: 1 to: 1266
KIBB8676.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 309 1266
Size: 309 957
Acc65I G'GTAC_C
Cuts at: 0 1197 1266
Size: 1197 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 1004 1266
Size: 362 642 262
Fragments arranged by size:
642 362 262
AflII C'TTAA_G
Cuts at: 0 259 1266
Size: 259 1007
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 878 910 1266
Size: 878 32 356
Fragments arranged by size:
878 356 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1049 1266
Size: 1049 217
ApaI G_GGCC'C
Cuts at: 0 380 1266
Size: 380 886
ApoI r'AATT_y
Cuts at: 0 718 1266
Size: 718 548
AvaI C'yCGr_G
Cuts at: 0 833 1266
Size: 833 433
AvrII C'CTAG_G
Cuts at: 0 208 1266
Size: 208 1058
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 961 994 1266
Size: 961 33 272
Fragments arranged by size:
961 272 33
BamHI G'GATC_C
Cuts at: 0 938 1266
Size: 938 328
BanI G'GyrC_C
Cuts at: 0 321 1197 1266
Size: 321 876 69
Fragments arranged by size:
876 321 69
BanII G_rGCy'C
Cuts at: 0 380 464 1266
Size: 380 84 802
Fragments arranged by size:
802 380 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 646 1266
Size: 646 620
BciVI GTATCCnnnnn_n'
Cuts at: 0 989 1266
Size: 989 277
BlpI GC'TnA_GC
Cuts at: 0 311 794 1266
Size: 311 483 472
Fragments arranged by size:
483 472 311
Bme1580I G_kGCm'C
Cuts at: 0 380 1266
Size: 380 886
BmrI ACTGGGnnnn_n'
Cuts at: 0 413 1037 1266
Size: 413 624 229
Fragments arranged by size:
624 413 229
BmtI G_CTAG'C
Cuts at: 0 911 1266
Size: 911 355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 354 1266
Size: 354 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1266
Size: 370 896
Bpu10I CC'TnA_GC
Cuts at: 0 217 1266
Size: 217 1049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 421 853 1266
Size: 167 254 432 413
Fragments arranged by size:
432 413 254 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 894 1032 1266
Size: 894 138 234
Fragments arranged by size:
894 234 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2 32 52 82 230 260 878 908
Size: 2 30 20 30 148 30 618 30
Cuts at: 908 1266
Size: 358
Fragments arranged by size:
618 358 148 30 30 30 30 20
2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 837 1266
Size: 837 429
BseYI C'CCAG_C
Cuts at: 0 221 457 1266
Size: 221 236 809
Fragments arranged by size:
809 236 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 553 1035 1266
Size: 534 19 482 231
Fragments arranged by size:
534 482 231 19
BsiHKAI G_wGCw'C
Cuts at: 0 972 1266
Size: 972 294
BsmI GAATG_Cn'
Cuts at: 0 31 1266
Size: 31 1235
Bsp1286I G_dGCh'C
Cuts at: 0 380 464 972 1266
Size: 380 84 508 294
Fragments arranged by size:
508 380 294 84
BspHI T'CATG_A
Cuts at: 0 1031 1073 1266
Size: 1031 42 193
Fragments arranged by size:
1031 193 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 309 611 690 912 1266
Size: 309 302 79 222 354
Fragments arranged by size:
354 309 302 222 79
BsrDI GCAATG_nn'
Cuts at: 0 188 1266
Size: 188 1078
BsrFI r'CCGG_y
Cuts at: 0 1222 1266
Size: 1222 44
BsrGI T'GTAC_A
Cuts at: 0 1064 1266
Size: 1064 202
BstYI r'GATC_y
Cuts at: 0 938 1266
Size: 938 328
BtgI C'CryG_G
Cuts at: 0 272 325 744 1266
Size: 272 53 419 522
Fragments arranged by size:
522 419 272 53
BtsI GCAGTG_nn'
Cuts at: 0 369 788 1266
Size: 369 419 478
Fragments arranged by size:
478 419 369
DraI TTT'AAA
Cuts at: 0 446 1266
Size: 446 820
EaeI y'GGCC_r
Cuts at: 0 1220 1266
Size: 1220 46
EarI CTCTTCn'nnn_
Cuts at: 0 1030 1249 1266
Size: 1030 219 17
Fragments arranged by size:
1030 219 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 370 1004 1266
Size: 362 8 634 262
Fragments arranged by size:
634 362 262 8
EcoO109I rG'GnC_Cy
Cuts at: 0 213 408 600 702 1266
Size: 213 195 192 102 564
Fragments arranged by size:
564 213 195 192 102
EcoRI G'AATT_C
Cuts at: 0 718 1266
Size: 718 548
HaeII r_GCGC'y
Cuts at: 0 715 1092 1266
Size: 715 377 174
Fragments arranged by size:
715 377 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 827 859 908 913 940 945 977 1096
Size: 827 32 49 5 27 5 32 119
Cuts at: 1096 1128 1266
Size: 32 138
Fragments arranged by size:
827 138 119 49 32 32 32 27
5 5
KpnI G_GTAC'C
Cuts at: 0 1201 1266
Size: 1201 65
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1266
Size: 560 706
MslI CAynn'nnrTG
Cuts at: 0 749 1231 1266
Size: 749 482 35
Fragments arranged by size:
749 482 35
MspA1I CmG'CkG
Cuts at: 0 327 457 537 1216 1266
Size: 327 130 80 679 50
Fragments arranged by size:
679 327 130 80 50
NaeI GCC'GGC
Cuts at: 0 1224 1266
Size: 1224 42
NcoI C'CATG_G
Cuts at: 0 744 1266
Size: 744 522
NgoMIV G'CCGG_C
Cuts at: 0 1222 1266
Size: 1222 44
NheI G'CTAG_C
Cuts at: 0 907 1266
Size: 907 359
NspI r_CATG'y
Cuts at: 0 1263 1266
Size: 1263 3
PasI CC'CwG_GG
Cuts at: 0 594 1266
Size: 594 672
PfoI T'CCnGG_A
Cuts at: 0 20 56 1266
Size: 20 36 1210
Fragments arranged by size:
1210 36 20
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 878 910 1266
Size: 878 32 356
Fragments arranged by size:
878 356 32
PpuMI rG'GwC_Cy
Cuts at: 0 213 408 600 702 1266
Size: 213 195 192 102 564
Fragments arranged by size:
564 213 195 192 102
PspOMI G'GGCC_C
Cuts at: 0 376 1266
Size: 376 890
PstI C_TGCA'G
Cuts at: 0 320 728 1161 1266
Size: 320 408 433 105
Fragments arranged by size:
433 408 320 105
PvuII CAG'CTG
Cuts at: 0 457 537 1216 1266
Size: 457 80 679 50
Fragments arranged by size:
679 457 80 50
SacII CC_GC'GG
Cuts at: 0 328 1266
Size: 328 938
SanDI GG'GwC_CC
Cuts at: 0 408 1266
Size: 408 858
SbfI CC_TGCA'GG
Cuts at: 0 728 1266
Size: 728 538
SexAI A'CCwGG_T
Cuts at: 0 527 1266
Size: 527 739
SfcI C'TryA_G
Cuts at: 0 316 724 1157 1254 1266
Size: 316 408 433 97 12
Fragments arranged by size:
433 408 316 97 12
SmlI C'TyrA_G
Cuts at: 0 182 259 436 868 1266
Size: 182 77 177 432 398
Fragments arranged by size:
432 398 182 177 77
SphI G_CATG'C
Cuts at: 0 1263 1266
Size: 1263 3
SspI AAT'ATT
Cuts at: 0 768 1266
Size: 768 498
StuI AGG'CCT
Cuts at: 0 812 1266
Size: 812 454
StyI C'CwwG_G
Cuts at: 0 208 744 1266
Size: 208 536 522
Fragments arranged by size:
536 522 208
TatI w'GTAC_w
Cuts at: 0 1064 1266
Size: 1064 202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 230 262 1266
Size: 2 32 196 32 1004
Fragments arranged by size:
1004 196 32 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 303 1266
Size: 303 963
XmnI GAAnn'nnTTC
Cuts at: 0 281 890 1266
Size: 281 609 376
Fragments arranged by size:
609 376 281
Enzymes that do cut:
AarI Acc65I AcuI AflII AloI AlwNI ApaI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbsI BciVI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BstYI BtgI BtsI
DraI EaeI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I
KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV NheI
NspI PasI PfoI PpiI PpuMI PspOMI PstI PvuII
SacII SanDI SbfI SexAI SfcI SmlI SphI SspI
StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI BbeI BbvCI BcgI
BclI BfrBI BglI BglII BmgBI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MscI NarI NdeI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SapI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI