Restriction map for
pF1KB8680
(Linear) (Six-base) MAPSORT of: KIBB8680.seq Check: 727 from: 1 to: 1899
KIBB8680.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AatII G_ACGT'C
Cuts at: 0 1808 1899
Size: 1808 91
AccI GT'mk_AC
Cuts at: 0 875 1899
Size: 875 1024
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1028 1262 1712 1899
Size: 770 258 234 450 187
Fragments arranged by size:
770 450 258 234 187
AflIII A'CryG_T
Cuts at: 0 1073 1578 1899
Size: 1073 505 321
Fragments arranged by size:
1073 505 321
AleI CACnn'nnGTG
Cuts at: 0 818 1899
Size: 818 1081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1237 1462 1899
Size: 1237 225 437
Fragments arranged by size:
1237 437 225
AlwNI CAG_nnn'CTG
Cuts at: 0 136 1899
Size: 136 1763
ApaLI G'TGCA_C
Cuts at: 0 74 1899
Size: 74 1825
AvaI C'yCGr_G
Cuts at: 0 424 1899
Size: 424 1475
BanI G'GyrC_C
Cuts at: 0 1859 1899
Size: 1859 40
BanII G_rGCy'C
Cuts at: 0 425 443 1899
Size: 425 18 1456
Fragments arranged by size:
1456 425 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 356 872 1415 1899
Size: 356 516 543 484
Fragments arranged by size:
543 516 484 356
BbvCI CC'TCA_GC
Cuts at: 0 379 434 614 1899
Size: 379 55 180 1285
Fragments arranged by size:
1285 379 180 55
BclI T'GATC_A
Cuts at: 0 951 1167 1899
Size: 951 216 732
Fragments arranged by size:
951 732 216
BfrBI ATG'CAT
Cuts at: 0 929 1899
Size: 929 970
BlpI GC'TnA_GC
Cuts at: 0 652 1210 1435 1899
Size: 652 558 225 464
Fragments arranged by size:
652 558 464 225
Bme1580I G_kGCm'C
Cuts at: 0 78 1899
Size: 78 1821
BmgBI CAC'GTC
Cuts at: 0 1599 1824 1899
Size: 1599 225 75
Fragments arranged by size:
1599 225 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 483 1899
Size: 483 1416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 799 1899
Size: 532 267 1100
Fragments arranged by size:
1100 532 267
Bpu10I CC'TnA_GC
Cuts at: 0 379 434 442 614 1899
Size: 379 55 8 172 1285
Fragments arranged by size:
1285 379 172 55 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1899
Size: 287 1612
BsaHI Gr'CG_yC
Cuts at: 0 1805 1899
Size: 1805 94
BsaWI w'CCGG_w
Cuts at: 0 193 564 1899
Size: 193 371 1335
Fragments arranged by size:
1335 371 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 581 611 1899
Size: 581 30 1288
Fragments arranged by size:
1288 581 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 451 547 712 796 1027 1899
Size: 433 18 96 165 84 231 872
Fragments arranged by size:
872 433 231 165 96 84 18
BseYI C'CCAG_C
Cuts at: 0 245 1899
Size: 245 1654
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1899
Size: 760 1139
BsiHKAI G_wGCw'C
Cuts at: 0 78 425 443 1105 1899
Size: 78 347 18 662 794
Fragments arranged by size:
794 662 347 78 18
Bsp1286I G_dGCh'C
Cuts at: 0 78 425 443 1105 1899
Size: 78 347 18 662 794
Fragments arranged by size:
794 662 347 78 18
BspEI T'CCGG_A
Cuts at: 0 564 1899
Size: 564 1335
BspHI T'CATG_A
Cuts at: 0 903 954 1899
Size: 903 51 945
Fragments arranged by size:
945 903 51
BsrBI CCG'CTC
Cuts at: 0 390 530 1899
Size: 390 140 1369
Fragments arranged by size:
1369 390 140
BsrDI GCAATG_nn'
Cuts at: 0 1339 1899
Size: 1339 560
BsrFI r'CCGG_y
Cuts at: 0 181 326 1899
Size: 181 145 1573
Fragments arranged by size:
1573 181 145
BstYI r'GATC_y
Cuts at: 0 123 1899
Size: 123 1776
Bsu36I CC'TnA_GG
Cuts at: 0 767 1466 1899
Size: 767 699 433
Fragments arranged by size:
767 699 433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 799 834 1899
Size: 799 35 1065
Fragments arranged by size:
1065 799 35
DraIII CAC_nnn'GTG
Cuts at: 0 97 1899
Size: 97 1802
EaeI y'GGCC_r
Cuts at: 0 320 1899
Size: 320 1579
EarI CTCTTCn'nnn_
Cuts at: 0 153 398 1004 1394 1679 1899
Size: 153 245 606 390 285 220
Fragments arranged by size:
606 390 285 245 220 153
EcoICRI GAG'CTC
Cuts at: 0 423 441 1899
Size: 423 18 1458
Fragments arranged by size:
1458 423 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 770 799 1028 1262 1712 1899
Size: 532 238 29 229 234 450 187
Fragments arranged by size:
532 450 238 234 229 187 29
EcoO109I rG'GnC_Cy
Cuts at: 0 46 552 594 1899
Size: 46 506 42 1305
Fragments arranged by size:
1305 506 46 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 427 1899
Size: 427 1472
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 581 613 883 915 1321 1353 1899
Size: 581 32 270 32 406 32 546
Fragments arranged by size:
581 546 406 270 32 32 32
HincII GTy'rAC
Cuts at: 0 876 1899
Size: 876 1023
HindIII A'AGCT_T
Cuts at: 0 409 1899
Size: 409 1490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 851 896 1899
Size: 151 700 45 1003
Fragments arranged by size:
1003 700 151 45
MscI TGG'CCA
Cuts at: 0 322 1899
Size: 322 1577
MslI CAynn'nnrTG
Cuts at: 0 818 1163 1899
Size: 818 345 736
Fragments arranged by size:
818 736 345
MspA1I CmG'CkG
Cuts at: 0 249 609 1899
Size: 249 360 1290
Fragments arranged by size:
1290 360 249
NaeI GCC'GGC
Cuts at: 0 183 1899
Size: 183 1716
NgoMIV G'CCGG_C
Cuts at: 0 181 1899
Size: 181 1718
NsiI A_TGCA'T
Cuts at: 0 931 1899
Size: 931 968
NspI r_CATG'y
Cuts at: 0 1077 1756 1899
Size: 1077 679 143
Fragments arranged by size:
1077 679 143
PasI CC'CwG_GG
Cuts at: 0 585 731 1899
Size: 585 146 1168
Fragments arranged by size:
1168 585 146
PciI A'CATG_T
Cuts at: 0 1073 1899
Size: 1073 826
PpuMI rG'GwC_Cy
Cuts at: 0 594 1899
Size: 594 1305
PstI C_TGCA'G
Cuts at: 0 1001 1899
Size: 1001 898
PvuII CAG'CTG
Cuts at: 0 249 609 1899
Size: 249 360 1290
Fragments arranged by size:
1290 360 249
SacI G_AGCT'C
Cuts at: 0 425 443 1899
Size: 425 18 1456
Fragments arranged by size:
1456 425 18
SalI G'TCGA_C
Cuts at: 0 874 1899
Size: 874 1025
SapI GCTCTTCn'nnn_
Cuts at: 0 1679 1899
Size: 1679 220
SfcI C'TryA_G
Cuts at: 0 997 1320 1899
Size: 997 323 579
Fragments arranged by size:
997 579 323
SmlI C'TyrA_G
Cuts at: 0 302 1899
Size: 302 1597
StyI C'CwwG_G
Cuts at: 0 187 1356 1413 1899
Size: 187 1169 57 486
Fragments arranged by size:
1169 486 187 57
TatI w'GTAC_w
Cuts at: 0 711 1769 1899
Size: 711 1058 130
Fragments arranged by size:
1058 711 130
Tth111I GACn'n_nGTC
Cuts at: 0 871 1899
Size: 871 1028
XbaI T'CTAG_A
Cuts at: 0 219 1899
Size: 219 1680
ZraI GAC'GTC
Cuts at: 0 1806 1899
Size: 1806 93
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlfI AlwNI ApaLI
AvaI BanI BanII BbsI BbvCI BclI BfrBI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BsrBI BsrDI BsrFI BstYI Bsu36I CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII
MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI
PasI PciI PpuMI PstI PvuII SacI SalI SapI
SfcI SmlI StyI TatI Tth111I XbaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BglI BglII BmtI BplI BsaI
BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspMI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI BtsI ClaI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NotI
NruI PacI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI XcmI XhoI XmaI XmnI