Restriction map for
pF1KB8681
(Linear) (Six-base) MAPSORT of: KIBB8681.seq Check: 7150 from: 1 to: 1131
KIBB8681.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 245 1131
Size: 245 886
Acc65I G'GTAC_C
Cuts at: 0 1085 1131
Size: 1085 46
AfeI AGC'GCT
Cuts at: 0 265 1131
Size: 265 866
AflIII A'CryG_T
Cuts at: 0 325 1131
Size: 325 806
AlwNI CAG_nnn'CTG
Cuts at: 0 84 543 1131
Size: 84 459 588
Fragments arranged by size:
588 459 84
ApaLI G'TGCA_C
Cuts at: 0 727 1131
Size: 727 404
AvaI C'yCGr_G
Cuts at: 0 132 1131
Size: 132 999
BanI G'GyrC_C
Cuts at: 0 233 441 823 887 1085 1131
Size: 233 208 382 64 198 46
Fragments arranged by size:
382 233 208 198 64 46
BanII G_rGCy'C
Cuts at: 0 139 166 207 1131
Size: 139 27 41 924
Fragments arranged by size:
924 139 41 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 383 1131
Size: 349 34 748
Fragments arranged by size:
748 349 34
BfrBI ATG'CAT
Cuts at: 0 1045 1131
Size: 1045 86
BglI GCCn_nnn'nGGC
Cuts at: 0 107 577 1131
Size: 107 470 554
Fragments arranged by size:
554 470 107
BlpI GC'TnA_GC
Cuts at: 0 267 1131
Size: 267 864
Bme1580I G_kGCm'C
Cuts at: 0 446 731 1131
Size: 446 285 400
Fragments arranged by size:
446 400 285
BmgBI CAC'GTC
Cuts at: 0 326 1131
Size: 326 805
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 373 1131
Size: 373 758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 880 1131
Size: 78 802 251
Fragments arranged by size:
802 251 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 862 1131
Size: 499 363 269
Fragments arranged by size:
499 363 269
BsaHI Gr'CG_yC
Cuts at: 0 775 1131
Size: 775 356
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 682 712 721 751 1131
Size: 682 30 9 30 380
Fragments arranged by size:
682 380 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 417 483 703 1131
Size: 417 66 220 428
Fragments arranged by size:
428 417 220 66
BseYI C'CCAG_C
Cuts at: 0 63 77 174 567 653 1122 1131
Size: 63 14 97 393 86 469 9
Fragments arranged by size:
469 393 97 86 63 14 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1131
Size: 866 265
BsiHKAI G_wGCw'C
Cuts at: 0 731 1131
Size: 731 400
BsmI GAATG_Cn'
Cuts at: 0 1045 1131
Size: 1045 86
Bsp1286I G_dGCh'C
Cuts at: 0 139 166 207 446 731 1131
Size: 139 27 41 239 285 400
Fragments arranged by size:
400 285 239 139 41 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 245 1097 1131
Size: 245 852 34
Fragments arranged by size:
852 245 34
BsrFI r'CCGG_y
Cuts at: 0 962 1131
Size: 962 169
BssHII G'CGCG_C
Cuts at: 0 240 1131
Size: 240 891
BstEII G'GTnAC_C
Cuts at: 0 603 1131
Size: 603 528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 840 1131
Size: 453 387 291
Fragments arranged by size:
453 387 291
BtgI C'CryG_G
Cuts at: 0 140 166 815 827 1131
Size: 140 26 649 12 304
Fragments arranged by size:
649 304 140 26 12
BtsI GCAGTG_nn'
Cuts at: 0 712 1131
Size: 712 419
DrdI GACnn_nn'nnGTC
Cuts at: 0 496 790 1131
Size: 496 294 341
Fragments arranged by size:
496 341 294
EaeI y'GGCC_r
Cuts at: 0 830 851 1131
Size: 830 21 280
Fragments arranged by size:
830 280 21
EarI CTCTTCn'nnn_
Cuts at: 0 542 1131
Size: 542 589
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 727 1131
Size: 727 404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 880 1131
Size: 78 802 251
Fragments arranged by size:
802 251 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 922 1131
Size: 922 209
EcoO109I rG'GnC_Cy
Cuts at: 0 249 915 1131
Size: 249 666 216
Fragments arranged by size:
666 249 216
FspI TGC'GCA
Cuts at: 0 406 1131
Size: 406 725
HaeII r_GCGC'y
Cuts at: 0 31 190 267 512 1131
Size: 31 159 77 245 619
Fragments arranged by size:
619 245 159 77 31
KpnI G_GTAC'C
Cuts at: 0 1089 1131
Size: 1089 42
MscI TGG'CCA
Cuts at: 0 832 1131
Size: 832 299
MslI CAynn'nnrTG
Cuts at: 0 451 1131
Size: 451 680
MspA1I CmG'CkG
Cuts at: 0 84 168 303 850 1131
Size: 84 84 135 547 281
Fragments arranged by size:
547 281 135 84 84
NsiI A_TGCA'T
Cuts at: 0 1047 1131
Size: 1047 84
PasI CC'CwG_GG
Cuts at: 0 195 207 1131
Size: 195 12 924
Fragments arranged by size:
924 195 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 1131
Size: 387 744
PfoI T'CCnGG_A
Cuts at: 0 896 1131
Size: 896 235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 790 822 1131
Size: 406 32 352 32 309
Fragments arranged by size:
406 352 309 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 249 915 1131
Size: 249 666 216
Fragments arranged by size:
666 249 216
PstI C_TGCA'G
Cuts at: 0 150 302 340 1131
Size: 150 152 38 791
Fragments arranged by size:
791 152 150 38
PvuII CAG'CTG
Cuts at: 0 303 1131
Size: 303 828
SacII CC_GC'GG
Cuts at: 0 169 1131
Size: 169 962
ScaI AGT'ACT
Cuts at: 0 625 1131
Size: 625 506
SexAI A'CCwGG_T
Cuts at: 0 380 524 1131
Size: 380 144 607
Fragments arranged by size:
607 380 144
SfcI C'TryA_G
Cuts at: 0 146 298 336 432 1131
Size: 146 152 38 96 699
Fragments arranged by size:
699 152 146 96 38
SmlI C'TyrA_G
Cuts at: 0 514 877 1131
Size: 514 363 254
Fragments arranged by size:
514 363 254
StuI AGG'CCT
Cuts at: 0 1117 1131
Size: 1117 14
StyI C'CwwG_G
Cuts at: 0 50 1131
Size: 50 1081
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 32 622 832 930 1131
Size: 32 590 210 98 201
Fragments arranged by size:
590 210 201 98 32
TatI w'GTAC_w
Cuts at: 0 317 623 836 1131
Size: 317 306 213 295
Fragments arranged by size:
317 306 295 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 682 714 721 753 1131
Size: 682 32 7 32 378
Fragments arranged by size:
682 378 32 32 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 661 768 1131
Size: 661 107 363
Fragments arranged by size:
661 363 107
Enzymes that do cut:
AarI Acc65I AfeI AflIII AlwNI ApaLI AvaI BanI
BanII BcgI BfrBI BglI BlpI Bme1580I BmgBI BplI
BpmI BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BssHII BstEII BstXI BtgI
BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII KpnI MscI MslI MspA1I NsiI PasI
PflMI PfoI PpiI PpuMI PstI PvuII SacII ScaI
SexAI SfcI SmlI StuI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AgeI AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BglII
BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI