Restriction map for
pF1KB8683
(Linear) (Six-base) MAPSORT of: KIBB8683.seq Check: 6902 from: 1 to: 2016
KIBB8683.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
Acc65I G'GTAC_C
Cuts at: 0 933 2016
Size: 933 1083
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 109 193 220 865 890 922 2016
Size: 90 19 84 27 645 25 32 1094
Fragments arranged by size:
1094 645 90 84 32 27 25 19
AflII C'TTAA_G
Cuts at: 0 441 2016
Size: 441 1575
AflIII A'CryG_T
Cuts at: 0 1142 2016
Size: 1142 874
AhdI GACnn_n'nnGTC
Cuts at: 0 1359 2016
Size: 1359 657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1328 2016
Size: 1328 688
AlwNI CAG_nnn'CTG
Cuts at: 0 570 2016
Size: 570 1446
ApoI r'AATT_y
Cuts at: 0 82 343 1123 1829 1876 2016
Size: 82 261 780 706 47 140
Fragments arranged by size:
780 706 261 140 82 47
AvaI C'yCGr_G
Cuts at: 0 1594 2016
Size: 1594 422
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1449 1482 2016
Size: 1449 33 534
Fragments arranged by size:
1449 534 33
BanI G'GyrC_C
Cuts at: 0 637 933 2016
Size: 637 296 1083
Fragments arranged by size:
1083 637 296
BanII G_rGCy'C
Cuts at: 0 524 581 1086 1595 1684 2016
Size: 524 57 505 509 89 332
Fragments arranged by size:
524 509 505 332 89 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 559 629 871 1192 2016
Size: 559 70 242 321 824
Fragments arranged by size:
824 559 321 242 70
BbvCI CC'TCA_GC
Cuts at: 0 209 328 482 2016
Size: 209 119 154 1534
Fragments arranged by size:
1534 209 154 119
BciVI GTATCCnnnnn_n'
Cuts at: 0 690 2016
Size: 690 1326
BclI T'GATC_A
Cuts at: 0 629 2016
Size: 629 1387
BglI GCCn_nnn'nGGC
Cuts at: 0 1243 2016
Size: 1243 773
BmrI ACTGGGnnnn_n'
Cuts at: 0 1954 2016
Size: 1954 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 436 735 853 1060 1201 1411 2016
Size: 205 231 299 118 207 141 210 605
Fragments arranged by size:
605 299 231 210 207 205 141 118
Bpu10I CC'TnA_GC
Cuts at: 0 209 328 482 2016
Size: 209 119 154 1534
Fragments arranged by size:
1534 209 154 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 808 2016
Size: 526 282 1208
Fragments arranged by size:
1208 526 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 2016
Size: 13 2003
BsaAI yAC'GTr
Cuts at: 0 585 1145 1497 2016
Size: 585 560 352 519
Fragments arranged by size:
585 560 519 352
BsaWI w'CCGG_w
Cuts at: 0 752 2016
Size: 752 1264
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1297 1590 2016
Size: 1297 293 426
Fragments arranged by size:
1297 426 293
BseYI C'CCAG_C
Cuts at: 0 231 470 996 1268 1683 2016
Size: 231 239 526 272 415 333
Fragments arranged by size:
526 415 333 272 239 231
BsiEI CG_ry'CG
Cuts at: 0 1212 2016
Size: 1212 804
BsiHKAI G_wGCw'C
Cuts at: 0 581 2016
Size: 581 1435
BsmI GAATG_Cn'
Cuts at: 0 356 987 1317 2016
Size: 356 631 330 699
Fragments arranged by size:
699 631 356 330
Bsp1286I G_dGCh'C
Cuts at: 0 524 581 1086 1595 1684 2016
Size: 524 57 505 509 89 332
Fragments arranged by size:
524 509 505 332 89 57
BspHI T'CATG_A
Cuts at: 0 674 2016
Size: 674 1342
BsrBI CCG'CTC
Cuts at: 0 63 747 2016
Size: 63 684 1269
Fragments arranged by size:
1269 684 63
BsrGI T'GTAC_A
Cuts at: 0 272 2016
Size: 272 1744
BssSI C'ACGA_G
Cuts at: 0 1201 2016
Size: 1201 815
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1255 1323 1497 2016
Size: 414 841 68 174 519
Fragments arranged by size:
841 519 414 174 68
BstYI r'GATC_y
Cuts at: 0 193 1157 2016
Size: 193 964 859
Fragments arranged by size:
964 859 193
BtgI C'CryG_G
Cuts at: 0 278 2016
Size: 278 1738
BtsI GCAGTG_nn'
Cuts at: 0 870 1279 1618 2016
Size: 870 409 339 398
Fragments arranged by size:
870 409 398 339
EaeI y'GGCC_r
Cuts at: 0 50 1209 2016
Size: 50 1159 807
Fragments arranged by size:
1159 807 50
EagI C'GGCC_G
Cuts at: 0 1209 2016
Size: 1209 807
EarI CTCTTCn'nnn_
Cuts at: 0 923 1809 1923 2016
Size: 923 886 114 93
Fragments arranged by size:
923 886 114 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 550 2016
Size: 550 1466
EcoICRI GAG'CTC
Cuts at: 0 579 2016
Size: 579 1437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 109 193 205 220 436 735 853
Size: 90 19 84 12 15 216 299 118
Cuts at: 853 865 890 922 1060 1201 1411 2016
Size: 12 25 32 138 141 210 605
Fragments arranged by size:
605 299 216 210 141 138 118 90
84 32 25 19 15 12 12
EcoO109I rG'GnC_Cy
Cuts at: 0 429 666 1035 1386 1749 2016
Size: 429 237 369 351 363 267
Fragments arranged by size:
429 369 363 351 267 237
EcoRI G'AATT_C
Cuts at: 0 343 1876 2016
Size: 343 1533 140
Fragments arranged by size:
1533 343 140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 419 895 2016
Size: 419 476 1121
Fragments arranged by size:
1121 476 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 321 353 1060 1092 1345 1377 2016
Size: 321 32 707 32 253 32 639
Fragments arranged by size:
707 639 321 253 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1402 1529 2016
Size: 1402 127 487
Fragments arranged by size:
1402 487 127
KpnI G_GTAC'C
Cuts at: 0 937 2016
Size: 937 1079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 2016
Size: 936 1080
MslI CAynn'nnrTG
Cuts at: 0 844 2016
Size: 844 1172
MspA1I CmG'CkG
Cuts at: 0 1214 2016
Size: 1214 802
NcoI C'CATG_G
Cuts at: 0 278 2016
Size: 278 1738
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 2016
Size: 570 1446
PmlI CAC'GTG
Cuts at: 0 1145 2016
Size: 1145 871
PpuMI rG'GwC_Cy
Cuts at: 0 429 666 1035 2016
Size: 429 237 369 981
Fragments arranged by size:
981 429 369 237
PstI C_TGCA'G
Cuts at: 0 1022 1679 2016
Size: 1022 657 337
Fragments arranged by size:
1022 657 337
SacI G_AGCT'C
Cuts at: 0 581 2016
Size: 581 1435
SfcI C'TryA_G
Cuts at: 0 1018 1675 2016
Size: 1018 657 341
Fragments arranged by size:
1018 657 341
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1243 2016
Size: 1243 773
SmlI C'TyrA_G
Cuts at: 0 441 541 823 2016
Size: 441 100 282 1193
Fragments arranged by size:
1193 441 282 100
StuI AGG'CCT
Cuts at: 0 333 535 1237 1842 2016
Size: 333 202 702 605 174
Fragments arranged by size:
702 605 333 202 174
StyI C'CwwG_G
Cuts at: 0 278 299 512 1049 1445 1745 2016
Size: 278 21 213 537 396 300 271
Fragments arranged by size:
537 396 300 278 271 213 21
TatI w'GTAC_w
Cuts at: 0 272 2016
Size: 272 1744
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1285 1317 2016
Size: 1285 32 699
Fragments arranged by size:
1285 699 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1323 1728 2016
Size: 1323 405 288
Fragments arranged by size:
1323 405 288
XmnI GAAnn'nnTTC
Cuts at: 0 399 2016
Size: 399 1617
Enzymes that do cut:
Acc65I AcuI AflII AflIII AhdI AlfI AlwNI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI
BglI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrGI BssSI BstXI BstYI BtgI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I
HindIII KpnI MmeI MslI MspA1I NcoI PflMI PmlI
PpuMI PstI SacI SfcI SfiI SmlI StuI StyI
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BcgI BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI
BsaBI BsaHI BsaXI BsgI BsiWI BsmBI BspEI BspMI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI