Restriction map for
pF1KB8684
(Linear) (Six-base) MAPSORT of: KIBB8684.seq Check: 4032 from: 1 to: 1137
KIBB8684.orf
With 182 enzymes: SgfI *
July 15, 2008 09:38 ..
AclI AA'CG_TT
Cuts at: 0 317 1137
Size: 317 820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1137
Size: 434 703
AflIII A'CryG_T
Cuts at: 0 792 1137
Size: 792 345
AhdI GACnn_n'nnGTC
Cuts at: 0 265 1137
Size: 265 872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 320 1137
Size: 320 817
AlwNI CAG_nnn'CTG
Cuts at: 0 80 1137
Size: 80 1057
ApoI r'AATT_y
Cuts at: 0 337 922 1137
Size: 337 585 215
Fragments arranged by size:
585 337 215
AseI AT'TA_AT
Cuts at: 0 250 1137
Size: 250 887
BbsI GAAGACnn'nnnn_
Cuts at: 0 902 1137
Size: 902 235
BfrBI ATG'CAT
Cuts at: 0 22 152 1137
Size: 22 130 985
Fragments arranged by size:
985 130 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 463 1137
Size: 463 674
Bpu10I CC'TnA_GC
Cuts at: 0 994 1137
Size: 994 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 712 1137
Size: 627 85 425
Fragments arranged by size:
627 425 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 252 1137
Size: 252 885
BsaAI yAC'GTr
Cuts at: 0 144 1137
Size: 144 993
BsaBI GATnn'nnATC
Cuts at: 0 1037 1137
Size: 1037 100
BsaHI Gr'CG_yC
Cuts at: 0 527 1137
Size: 527 610
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 446 476 1137
Size: 446 30 661
Fragments arranged by size:
661 446 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 756 1137
Size: 756 381
BsmI GAATG_Cn'
Cuts at: 0 91 1137
Size: 91 1046
BsrDI GCAATG_nn'
Cuts at: 0 24 1137
Size: 24 1113
BstAPI GCAn_nnn'nTGC
Cuts at: 0 104 1137
Size: 104 1033
BstYI r'GATC_y
Cuts at: 0 1045 1137
Size: 1045 92
Bsu36I CC'TnA_GG
Cuts at: 0 716 878 1137
Size: 716 162 259
Fragments arranged by size:
716 259 162
BtgI C'CryG_G
Cuts at: 0 530 1137
Size: 530 607
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 140 1137
Size: 140 997
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6 41 1137
Size: 6 35 1096
Fragments arranged by size:
1096 35 6
DraI TTT'AAA
Cuts at: 0 365 1137
Size: 365 772
DraIII CAC_nnn'GTG
Cuts at: 0 471 1137
Size: 471 666
EaeI y'GGCC_r
Cuts at: 0 457 1137
Size: 457 680
EarI CTCTTCn'nnn_
Cuts at: 0 733 779 802 1046 1137
Size: 733 46 23 244 91
Fragments arranged by size:
733 244 91 46 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 899 1137
Size: 899 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1137
Size: 434 703
EcoNI CCTnn'n_nnAGG
Cuts at: 0 914 1137
Size: 914 223
EcoO109I rG'GnC_Cy
Cuts at: 0 963 1137
Size: 963 174
HaeII r_GCGC'y
Cuts at: 0 776 1137
Size: 776 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 251 283 1137
Size: 251 32 854
Fragments arranged by size:
854 251 32
HindIII A'AGCT_T
Cuts at: 0 416 1126 1137
Size: 416 710 11
Fragments arranged by size:
710 416 11
MluI A'CGCG_T
Cuts at: 0 792 1137
Size: 792 345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1137
Size: 446 691
NsiI A_TGCA'T
Cuts at: 0 24 154 1137
Size: 24 130 983
Fragments arranged by size:
983 130 24
NspI r_CATG'y
Cuts at: 0 17 26 64 487 1137
Size: 17 9 38 423 650
Fragments arranged by size:
650 423 38 17 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 471 1137
Size: 471 666
PmlI CAC'GTG
Cuts at: 0 144 1137
Size: 144 993
PstI C_TGCA'G
Cuts at: 0 735 1137
Size: 735 402
ScaI AGT'ACT
Cuts at: 0 1018 1137
Size: 1018 119
SexAI A'CCwGG_T
Cuts at: 0 392 1137
Size: 392 745
SfcI C'TryA_G
Cuts at: 0 731 1137
Size: 731 406
SmlI C'TyrA_G
Cuts at: 0 642 691 1137
Size: 642 49 446
Fragments arranged by size:
642 446 49
StyI C'CwwG_G
Cuts at: 0 500 1137
Size: 500 637
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 482 1137
Size: 482 655
TatI w'GTAC_w
Cuts at: 0 1016 1137
Size: 1016 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 489 521 1137
Size: 382 32 75 32 616
Fragments arranged by size:
616 382 75 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1120 1137
Size: 1120 17
XmnI GAAnn'nnTTC
Cuts at: 0 383 1137
Size: 383 754
Enzymes that do cut:
AclI AcuI AflIII AhdI AlfI AlwNI ApoI AseI
BbsI BfrBI BmrI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BsmI BsrDI BstAPI BstYI Bsu36I
BtgI BtgZI CspCI DraI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MluI MmeI
NsiI NspI PflMI PmlI PstI ScaI SexAI SfcI
SmlI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI
BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtsI ClaI DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XcmI XhoI XmaI ZraI