Restriction map for
pF1KB8686
(Linear) (Six-base) MAPSORT of: KIBB8686.seq Check: 2631 from: 1 to: 1212
KIBB8686.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1212
Size: 1115 97
AflIII A'CryG_T
Cuts at: 0 803 1212
Size: 803 409
AlwNI CAG_nnn'CTG
Cuts at: 0 42 1212
Size: 42 1170
ApoI r'AATT_y
Cuts at: 0 822 1065 1212
Size: 822 243 147
Fragments arranged by size:
822 243 147
AseI AT'TA_AT
Cuts at: 0 413 1212
Size: 413 799
AvaI C'yCGr_G
Cuts at: 0 81 1212
Size: 81 1131
BanII G_rGCy'C
Cuts at: 0 133 1212
Size: 133 1079
BbsI GAAGACnn'nnnn_
Cuts at: 0 751 1190 1212
Size: 751 439 22
Fragments arranged by size:
751 439 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 844 1212
Size: 844 368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 683 1212
Size: 64 619 529
Fragments arranged by size:
619 529 64
BsaBI GATnn'nnATC
Cuts at: 0 149 1212
Size: 149 1063
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 81 99 1212
Size: 81 18 1113
Fragments arranged by size:
1113 81 18
BseYI C'CCAG_C
Cuts at: 0 784 1212
Size: 784 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1212
Size: 770 442
BsiEI CG_ry'CG
Cuts at: 0 108 1212
Size: 108 1104
BsiHKAI G_wGCw'C
Cuts at: 0 133 690 1212
Size: 133 557 522
Fragments arranged by size:
557 522 133
Bsp1286I G_dGCh'C
Cuts at: 0 133 690 1212
Size: 133 557 522
Fragments arranged by size:
557 522 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 66 1212
Size: 66 1146
BsrBI CCG'CTC
Cuts at: 0 48 89 1212
Size: 48 41 1123
Fragments arranged by size:
1123 48 41
BsrDI GCAATG_nn'
Cuts at: 0 866 1212
Size: 866 346
BstBI TT'CG_AA
Cuts at: 0 148 1212
Size: 148 1064
BstYI r'GATC_y
Cuts at: 0 788 1212
Size: 788 424
BtsI GCAGTG_nn'
Cuts at: 0 961 1212
Size: 961 251
EaeI y'GGCC_r
Cuts at: 0 105 1193 1212
Size: 105 1088 19
Fragments arranged by size:
1088 105 19
EagI C'GGCC_G
Cuts at: 0 105 1212
Size: 105 1107
EarI CTCTTCn'nnn_
Cuts at: 0 983 989 1046 1212
Size: 983 6 57 166
Fragments arranged by size:
983 166 57 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1212
Size: 19 1193
EcoICRI GAG'CTC
Cuts at: 0 131 1212
Size: 131 1081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 683 1115 1212
Size: 64 619 432 97
Fragments arranged by size:
619 432 97 64
EcoO109I rG'GnC_Cy
Cuts at: 0 78 1212
Size: 78 1134
HaeII r_GCGC'y
Cuts at: 0 21 1212
Size: 21 1191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1212
Size: 1021 32 159
Fragments arranged by size:
1021 159 32
HincII GTy'rAC
Cuts at: 0 1154 1212
Size: 1154 58
HindIII A'AGCT_T
Cuts at: 0 1176 1212
Size: 1176 36
HpaI GTT'AAC
Cuts at: 0 1154 1212
Size: 1154 58
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 1212
Size: 378 834
MscI TGG'CCA
Cuts at: 0 1195 1212
Size: 1195 17
MspA1I CmG'CkG
Cuts at: 0 30 1212
Size: 30 1182
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 1212
Size: 42 1170
PfoI T'CCnGG_A
Cuts at: 0 35 660 1212
Size: 35 625 552
Fragments arranged by size:
625 552 35
PpuMI rG'GwC_Cy
Cuts at: 0 78 1212
Size: 78 1134
PsiI TTA'TAA
Cuts at: 0 655 1212
Size: 655 557
PstI C_TGCA'G
Cuts at: 0 26 77 1212
Size: 26 51 1135
Fragments arranged by size:
1135 51 26
SacI G_AGCT'C
Cuts at: 0 133 1212
Size: 133 1079
SfcI C'TryA_G
Cuts at: 0 22 73 156 1212
Size: 22 51 83 1056
Fragments arranged by size:
1056 83 51 22
SspI AAT'ATT
Cuts at: 0 714 1212
Size: 714 498
StyI C'CwwG_G
Cuts at: 0 846 1212
Size: 846 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 1212
Size: 763 449
TatI w'GTAC_w
Cuts at: 0 435 795 1212
Size: 435 360 417
Fragments arranged by size:
435 417 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1091 1123 1212
Size: 1091 32 89
Fragments arranged by size:
1091 89 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 774 1212
Size: 774 438
XmnI GAAnn'nnTTC
Cuts at: 0 467 1212
Size: 467 745
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AseI AvaI BanII BbsI
BciVI BpmI BsaBI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BstBI BstYI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII HindIII HpaI MmeI MscI MspA1I PflMI PfoI
PpuMI PsiI PstI SacI SfcI SspI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI KasI KpnI MfeI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI