Restriction map for
pF1KB8687
(Linear) (Six-base) MAPSORT of: KIBB8687.seq Check: 6804 from: 1 to: 1944
KIBB8687.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1944
Size: 1180 764
AflIII A'CryG_T
Cuts at: 0 1671 1944
Size: 1671 273
AhdI GACnn_n'nnGTC
Cuts at: 0 1654 1944
Size: 1654 290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 373 1944
Size: 373 1571
AlwNI CAG_nnn'CTG
Cuts at: 0 118 1944
Size: 118 1826
ApoI r'AATT_y
Cuts at: 0 338 657 1944
Size: 338 319 1287
Fragments arranged by size:
1287 338 319
AseI AT'TA_AT
Cuts at: 0 1808 1944
Size: 1808 136
AvaI C'yCGr_G
Cuts at: 0 71 974 1944
Size: 71 903 970
Fragments arranged by size:
970 903 71
BanI G'GyrC_C
Cuts at: 0 157 1944
Size: 157 1787
BanII G_rGCy'C
Cuts at: 0 21 1944
Size: 21 1923
BbsI GAAGACnn'nnnn_
Cuts at: 0 1467 1944
Size: 1467 477
BglII A'GATC_T
Cuts at: 0 840 1944
Size: 840 1104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1944
Size: 171 1773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1904 1944
Size: 1904 40
BsaAI yAC'GTr
Cuts at: 0 35 1713 1944
Size: 35 1678 231
Fragments arranged by size:
1678 231 35
BsaBI GATnn'nnATC
Cuts at: 0 518 1944
Size: 518 1426
BsaHI Gr'CG_yC
Cuts at: 0 734 1944
Size: 734 1210
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1026 1056 1737 1767 1944
Size: 1026 30 681 30 177
Fragments arranged by size:
1026 681 177 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 29 99 112 1858 1944
Size: 29 70 13 1746 86
Fragments arranged by size:
1746 86 70 29 13
BseYI C'CCAG_C
Cuts at: 0 922 1944
Size: 922 1022
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 1944
Size: 705 1239
BsiEI CG_ry'CG
Cuts at: 0 252 1944
Size: 252 1692
BsiHKAI G_wGCw'C
Cuts at: 0 21 1944
Size: 21 1923
BsmI GAATG_Cn'
Cuts at: 0 430 442 1944
Size: 430 12 1502
Fragments arranged by size:
1502 430 12
Bsp1286I G_dGCh'C
Cuts at: 0 21 1944
Size: 21 1923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 769 1944
Size: 727 42 1175
Fragments arranged by size:
1175 727 42
BsrDI GCAATG_nn'
Cuts at: 0 362 1944
Size: 362 1582
BsrFI r'CCGG_y
Cuts at: 0 90 1944
Size: 90 1854
BssSI C'ACGA_G
Cuts at: 0 193 1944
Size: 193 1751
BstXI CCAn_nnnn'nTGG
Cuts at: 0 235 1944
Size: 235 1709
BstYI r'GATC_y
Cuts at: 0 840 1359 1944
Size: 840 519 585
Fragments arranged by size:
840 585 519
Bsu36I CC'TnA_GG
Cuts at: 0 119 752 1944
Size: 119 633 1192
Fragments arranged by size:
1192 633 119
BtgI C'CryG_G
Cuts at: 0 9 179 1693 1944
Size: 9 170 1514 251
Fragments arranged by size:
1514 251 170 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1288 1944
Size: 1288 656
BtsI GCAGTG_nn'
Cuts at: 0 383 605 1944
Size: 383 222 1339
Fragments arranged by size:
1339 383 222
DraI TTT'AAA
Cuts at: 0 1599 1944
Size: 1599 345
EaeI y'GGCC_r
Cuts at: 0 182 502 1944
Size: 182 320 1442
Fragments arranged by size:
1442 320 182
EarI CTCTTCn'nnn_
Cuts at: 0 217 1073 1406 1731 1944
Size: 217 856 333 325 213
Fragments arranged by size:
856 333 325 217 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 1944
Size: 109 1835
EcoICRI GAG'CTC
Cuts at: 0 19 1944
Size: 19 1925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1180 1944
Size: 171 1009 764
Fragments arranged by size:
1009 764 171
EcoO109I rG'GnC_Cy
Cuts at: 0 115 167 1944
Size: 115 52 1777
Fragments arranged by size:
1777 115 52
EcoRV GAT'ATC
Cuts at: 0 1101 1944
Size: 1101 843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 246 606 1944
Size: 246 360 1338
Fragments arranged by size:
1338 360 246
HaeII r_GCGC'y
Cuts at: 0 79 1944
Size: 79 1865
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 1492 1524 1624 1640 1656 1672
Size: 116 32 1344 32 100 16 16 16
Cuts at: 1672 1837 1869 1944
Size: 165 32 75
Fragments arranged by size:
1344 165 116 100 75 32 32 32
16 16 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 471 625 1184 1944
Size: 438 33 154 559 760
Fragments arranged by size:
760 559 438 154 33
MslI CAynn'nnrTG
Cuts at: 0 813 1944
Size: 813 1131
MspA1I CmG'CkG
Cuts at: 0 187 207 1944
Size: 187 20 1737
Fragments arranged by size:
1737 187 20
NaeI GCC'GGC
Cuts at: 0 92 1944
Size: 92 1852
NgoMIV G'CCGG_C
Cuts at: 0 90 1944
Size: 90 1854
NspI r_CATG'y
Cuts at: 0 957 1431 1675 1944
Size: 957 474 244 269
Fragments arranged by size:
957 474 269 244
PciI A'CATG_T
Cuts at: 0 1671 1944
Size: 1671 273
PfoI T'CCnGG_A
Cuts at: 0 1116 1944
Size: 1116 828
PpuMI rG'GwC_Cy
Cuts at: 0 115 1944
Size: 115 1829
PsiI TTA'TAA
Cuts at: 0 596 1944
Size: 596 1348
SacI G_AGCT'C
Cuts at: 0 21 1944
Size: 21 1923
SapI GCTCTTCn'nnn_
Cuts at: 0 217 1073 1731 1944
Size: 217 856 658 213
Fragments arranged by size:
856 658 217 213
SfcI C'TryA_G
Cuts at: 0 1441 1944
Size: 1441 503
SmlI C'TyrA_G
Cuts at: 0 1919 1944
Size: 1919 25
SnaBI TAC'GTA
Cuts at: 0 1713 1944
Size: 1713 231
SspI AAT'ATT
Cuts at: 0 1015 1701 1812 1944
Size: 1015 686 111 132
Fragments arranged by size:
1015 686 132 111
StuI AGG'CCT
Cuts at: 0 28 1944
Size: 28 1916
StyI C'CwwG_G
Cuts at: 0 23 1944
Size: 23 1921
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 1944
Size: 238 1706
TatI w'GTAC_w
Cuts at: 0 283 1944
Size: 283 1661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1026 1058 1737 1769 1944
Size: 1026 32 679 32 175
Fragments arranged by size:
1026 679 175 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 66 1465 1944
Size: 66 1399 479
Fragments arranged by size:
1399 479 66
XbaI T'CTAG_A
Cuts at: 0 1642 1944
Size: 1642 302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 454 512 1944
Size: 454 58 1432
Fragments arranged by size:
1432 454 58
Enzymes that do cut:
AcuI AflIII AhdI AlfI AlwNI ApoI AseI AvaI
BanI BanII BbsI BglII BpmI BpuEI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I MmeI MslI
MspA1I NaeI NgoMIV NspI PciI PfoI PpuMI PsiI
SacI SapI SfcI SmlI SnaBI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I ClaI CspCI DraIII DrdI
EagI EcoNI EcoRI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI SwaI XhoI XmaI XmnI ZraI