Restriction map for
pF1KB8690
(Linear) (Six-base) MAPSORT of: KIBB8690.seq Check: 5168 from: 1 to: 1833
KIBB8690.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 704 1833
Size: 704 1129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1833
Size: 91 1742
AhdI GACnn_n'nnGTC
Cuts at: 0 1703 1833
Size: 1703 130
AlwNI CAG_nnn'CTG
Cuts at: 0 14 341 414 696 1833
Size: 14 327 73 282 1137
Fragments arranged by size:
1137 327 282 73 14
ApaI G_GGCC'C
Cuts at: 0 121 963 1833
Size: 121 842 870
Fragments arranged by size:
870 842 121
ApaLI G'TGCA_C
Cuts at: 0 1454 1833
Size: 1454 379
AvaI C'yCGr_G
Cuts at: 0 121 174 448 458 574 675 1056 1228
Size: 121 53 274 10 116 101 381 172
Cuts at: 1228 1241 1473 1833
Size: 13 232 360
Fragments arranged by size:
381 360 274 232 172 121 116 101
53 13 10
BanI G'GyrC_C
Cuts at: 0 261 600 976 1068 1491 1833
Size: 261 339 376 92 423 342
Fragments arranged by size:
423 376 342 339 261 92
BanII G_rGCy'C
Cuts at: 0 121 797 963 1480 1575 1609 1833
Size: 121 676 166 517 95 34 224
Fragments arranged by size:
676 517 224 166 121 95 34
BbeI G_GCGC'C
Cuts at: 0 1072 1833
Size: 1072 761
BbsI GAAGACnn'nnnn_
Cuts at: 0 1757 1833
Size: 1757 76
BbvCI CC'TCA_GC
Cuts at: 0 1646 1833
Size: 1646 187
BglI GCCn_nnn'nGGC
Cuts at: 0 116 1833
Size: 116 1717
BlpI GC'TnA_GC
Cuts at: 0 1303 1608 1833
Size: 1303 305 225
Fragments arranged by size:
1303 305 225
Bme1580I G_kGCm'C
Cuts at: 0 121 181 647 963 1458 1786 1833
Size: 121 60 466 316 495 328 47
Fragments arranged by size:
495 466 328 316 121 60 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1042 1833
Size: 1042 791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 217 238 370 574 844 1330 1447
Size: 39 178 21 132 204 270 486 117
Cuts at: 1447 1833
Size: 386
Fragments arranged by size:
486 386 270 204 178 132 117 39
21
Bpu10I CC'TnA_GC
Cuts at: 0 1646 1833
Size: 1646 187
BsaHI Gr'CG_yC
Cuts at: 0 143 637 1069 1640 1833
Size: 143 494 432 571 193
Fragments arranged by size:
571 494 432 193 143
BsaWI w'CCGG_w
Cuts at: 0 1149 1833
Size: 1149 684
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1507 1537 1543 1573 1833
Size: 1507 30 6 30 260
Fragments arranged by size:
1507 260 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 778 841 1020 1125 1444 1521 1647
Size: 562 216 63 179 105 319 77 126
Cuts at: 1647 1734 1833
Size: 87 99
Fragments arranged by size:
562 319 216 179 126 105 99 87
77 63
BseYI C'CCAG_C
Cuts at: 0 517 1049 1139 1289 1787 1833
Size: 517 532 90 150 498 46
Fragments arranged by size:
532 517 498 150 90 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 1279 1833
Size: 1258 21 554
Fragments arranged by size:
1258 554 21
BsiEI CG_ry'CG
Cuts at: 0 107 1833
Size: 107 1726
BsiHKAI G_wGCw'C
Cuts at: 0 467 512 797 1458 1833
Size: 467 45 285 661 375
Fragments arranged by size:
661 467 375 285 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1197 1833
Size: 1197 636
Bsp1286I G_dGCh'C
Cuts at: 0 121 181 467 512 647 797 963 1458
Size: 121 60 286 45 135 150 166 495
Cuts at: 1458 1480 1575 1609 1786 1833
Size: 22 95 34 177 47
Fragments arranged by size:
495 286 177 166 150 135 121 95
60 47 45 34 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 704 990 1833
Size: 704 286 843
Fragments arranged by size:
843 704 286
BsrBI CCG'CTC
Cuts at: 0 447 848 1591 1833
Size: 447 401 743 242
Fragments arranged by size:
743 447 401 242
BsrFI r'CCGG_y
Cuts at: 0 106 719 1220 1833
Size: 106 613 501 613
Fragments arranged by size:
613 613 501 106
BssHII G'CGCG_C
Cuts at: 0 92 1833
Size: 92 1741
BstAPI GCAn_nnn'nTGC
Cuts at: 0 696 1833
Size: 696 1137
BstEII G'GTnAC_C
Cuts at: 0 1223 1833
Size: 1223 610
BstXI CCAn_nnnn'nTGG
Cuts at: 0 888 1833
Size: 888 945
BstYI r'GATC_y
Cuts at: 0 842 1833
Size: 842 991
Bsu36I CC'TnA_GG
Cuts at: 0 23 1032 1802 1833
Size: 23 1009 770 31
Fragments arranged by size:
1009 770 31 23
BtgI C'CryG_G
Cuts at: 0 384 583 1124 1254 1833
Size: 384 199 541 130 579
Fragments arranged by size:
579 541 384 199 130
BtsI GCAGTG_nn'
Cuts at: 0 487 666 718 1017 1215 1270 1831 1833
Size: 487 179 52 299 198 55 561 2
Fragments arranged by size:
561 487 299 198 179 55 52 2
DraIII CAC_nnn'GTG
Cuts at: 0 805 1833
Size: 805 1028
EaeI y'GGCC_r
Cuts at: 0 104 749 1014 1182 1833
Size: 104 645 265 168 651
Fragments arranged by size:
651 645 265 168 104
EagI C'GGCC_G
Cuts at: 0 104 1833
Size: 104 1729
EarI CTCTTCn'nnn_
Cuts at: 0 166 1833
Size: 166 1667
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 270 1052 1833
Size: 270 782 781
Fragments arranged by size:
782 781 270
EcoICRI GAG'CTC
Cuts at: 0 795 1833
Size: 795 1038
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 91 217 238 370 574 844 1330
Size: 39 52 126 21 132 204 270 486
Cuts at: 1330 1447 1833
Size: 117 386
Fragments arranged by size:
486 386 270 204 132 126 117 52
39 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1662 1833
Size: 1662 171
EcoO109I rG'GnC_Cy
Cuts at: 0 27 118 455 865 959 960 1422 1728
Size: 27 91 337 410 94 1 462 306
Cuts at: 1728 1798 1833
Size: 70 35
Fragments arranged by size:
462 410 337 306 94 91 70 35
27 1
FseI GG_CCGG'CC
Cuts at: 0 110 1833
Size: 110 1723
HaeII r_GCGC'y
Cuts at: 0 591 615 1072 1833
Size: 591 24 457 761
Fragments arranged by size:
761 591 457 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1507 1539 1689 1693 1721 1725 1747 1779
Size: 1507 32 150 4 28 4 22 32
Cuts at: 1779 1833
Size: 54
Fragments arranged by size:
1507 150 54 32 32 28 22 4
4
KasI G'GCGC_C
Cuts at: 0 1068 1833
Size: 1068 765
MscI TGG'CCA
Cuts at: 0 751 1833
Size: 751 1082
MslI CAynn'nnrTG
Cuts at: 0 886 1833
Size: 886 947
MspA1I CmG'CkG
Cuts at: 0 423 539 585 969 1049 1256 1293 1543
Size: 423 116 46 384 80 207 37 250
Cuts at: 1543 1833
Size: 290
Fragments arranged by size:
423 384 290 250 207 116 80 46
37
NaeI GCC'GGC
Cuts at: 0 108 1833
Size: 108 1725
NarI GG'CG_CC
Cuts at: 0 1069 1833
Size: 1069 764
NgoMIV G'CCGG_C
Cuts at: 0 106 1833
Size: 106 1727
PasI CC'CwG_GG
Cuts at: 0 991 1833
Size: 991 842
PfoI T'CCnGG_A
Cuts at: 0 188 1833
Size: 188 1645
PpuMI rG'GwC_Cy
Cuts at: 0 455 1728 1833
Size: 455 1273 105
Fragments arranged by size:
1273 455 105
PshAI GACnn'nnGTC
Cuts at: 0 914 1833
Size: 914 919
PspOMI G'GGCC_C
Cuts at: 0 117 959 1833
Size: 117 842 874
Fragments arranged by size:
874 842 117
PspXI vC'TCGA_Gb
Cuts at: 0 448 675 1473 1833
Size: 448 227 798 360
Fragments arranged by size:
798 448 360 227
PstI C_TGCA'G
Cuts at: 0 692 1580 1833
Size: 692 888 253
Fragments arranged by size:
888 692 253
PvuII CAG'CTG
Cuts at: 0 1049 1293 1833
Size: 1049 244 540
Fragments arranged by size:
1049 540 244
SacI G_AGCT'C
Cuts at: 0 797 1833
Size: 797 1036
SacII CC_GC'GG
Cuts at: 0 586 1257 1833
Size: 586 671 576
Fragments arranged by size:
671 586 576
SanDI GG'GwC_CC
Cuts at: 0 455 1728 1833
Size: 455 1273 105
Fragments arranged by size:
1273 455 105
SapI GCTCTTCn'nnn_
Cuts at: 0 166 1833
Size: 166 1667
SfcI C'TryA_G
Cuts at: 0 688 1576 1833
Size: 688 888 257
Fragments arranged by size:
888 688 257
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 116 1833
Size: 116 1717
SfoI GGC'GCC
Cuts at: 0 1070 1833
Size: 1070 763
SmaI CCC'GGG
Cuts at: 0 176 1230 1243 1833
Size: 176 1054 13 590
Fragments arranged by size:
1054 590 176 13
SmlI C'TyrA_G
Cuts at: 0 448 675 1473 1833
Size: 448 227 798 360
Fragments arranged by size:
798 448 360 227
SrfI GCCC'GGGC
Cuts at: 0 1243 1833
Size: 1243 590
StyI C'CwwG_G
Cuts at: 0 299 746 1613 1833
Size: 299 447 867 220
Fragments arranged by size:
867 447 299 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 961 1210 1697 1833
Size: 961 249 487 136
Fragments arranged by size:
961 487 249 136
TatI w'GTAC_w
Cuts at: 0 371 1833
Size: 371 1462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 272 304 1045 1077 1833
Size: 272 32 741 32 756
Fragments arranged by size:
756 741 272 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 636 1761 1833
Size: 636 1125 72
Fragments arranged by size:
1125 636 72
XhoI C'TCGA_G
Cuts at: 0 448 675 1473 1833
Size: 448 227 798 360
Fragments arranged by size:
798 448 360 227
XmaI C'CCGG_G
Cuts at: 0 174 1228 1241 1833
Size: 174 1054 13 592
Fragments arranged by size:
1054 592 174 13
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BplI
BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I KasI MscI MslI MspA1I NaeI
NarI NgoMIV PasI PfoI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SanDI SapI SfcI SfiI
SfoI SmaI SmlI SrfI StyI TaqII TatI TstI
Tth111I XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaBI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssSI BstBI BstZ17I BtgZI
ClaI CspCI DraI DrdI EcoRI EcoRV FalI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PsiI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI XbaI XcmI XmnI
ZraI