Restriction map for
pF1KB8691
(Linear) (Six-base) MAPSORT of: KIBB8691.seq Check: 1736 from: 1 to: 2079
KIBB8691.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1574 2014 2079
Size: 1574 440 65
Fragments arranged by size:
1574 440 65
AlwNI CAG_nnn'CTG
Cuts at: 0 171 654 1126 2079
Size: 171 483 472 953
Fragments arranged by size:
953 483 472 171
ApaI G_GGCC'C
Cuts at: 0 202 435 2079
Size: 202 233 1644
Fragments arranged by size:
1644 233 202
ApoI r'AATT_y
Cuts at: 0 824 2079
Size: 824 1255
AvaI C'yCGr_G
Cuts at: 0 135 235 369 2079
Size: 135 100 134 1710
Fragments arranged by size:
1710 135 134 100
BamHI G'GATC_C
Cuts at: 0 248 2079
Size: 248 1831
BanI G'GyrC_C
Cuts at: 0 321 462 1031 2079
Size: 321 141 569 1048
Fragments arranged by size:
1048 569 321 141
BanII G_rGCy'C
Cuts at: 0 202 435 1201 2079
Size: 202 233 766 878
Fragments arranged by size:
878 766 233 202
BbeI G_GCGC'C
Cuts at: 0 325 466 2079
Size: 325 141 1613
Fragments arranged by size:
1613 325 141
BbsI GAAGACnn'nnnn_
Cuts at: 0 750 860 1280 1307 1562 1838 2079
Size: 750 110 420 27 255 276 241
Fragments arranged by size:
750 420 276 255 241 110 27
BclI T'GATC_A
Cuts at: 0 836 1265 2079
Size: 836 429 814
Fragments arranged by size:
836 814 429
BglII A'GATC_T
Cuts at: 0 1316 1352 2079
Size: 1316 36 727
Fragments arranged by size:
1316 727 36
Bme1580I G_kGCm'C
Cuts at: 0 202 435 2079
Size: 202 233 1644
Fragments arranged by size:
1644 233 202
BmgBI CAC'GTC
Cuts at: 0 847 1866 2079
Size: 847 1019 213
Fragments arranged by size:
1019 847 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 205 2079
Size: 205 1874
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1896 2079
Size: 1896 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 676 697 775 1185 2079
Size: 127 549 21 78 410 894
Fragments arranged by size:
894 549 410 127 78 21
Bpu10I CC'TnA_GC
Cuts at: 0 43 993 2079
Size: 43 950 1086
Fragments arranged by size:
1086 950 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 2079
Size: 703 1376
BsaI GGTCTCn'nnnn_
Cuts at: 0 758 1363 1381 1465 2079
Size: 758 605 18 84 614
Fragments arranged by size:
758 614 605 84 18
BsaBI GATnn'nnATC
Cuts at: 0 1873 2079
Size: 1873 206
BsaHI Gr'CG_yC
Cuts at: 0 322 463 1277 2079
Size: 322 141 814 802
Fragments arranged by size:
814 802 322 141
BsaWI w'CCGG_w
Cuts at: 0 2074 2079
Size: 2074 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 253 564 594 1975 2079
Size: 91 162 311 30 1381 104
Fragments arranged by size:
1381 311 162 104 91 30
BseYI C'CCAG_C
Cuts at: 0 125 287 2079
Size: 125 162 1792
Fragments arranged by size:
1792 162 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1550 2079
Size: 1550 529
BsmI GAATG_Cn'
Cuts at: 0 805 2079
Size: 805 1274
BsmBI CGTCTCn'nnnn_
Cuts at: 0 721 2079
Size: 721 1358
Bsp1286I G_dGCh'C
Cuts at: 0 202 435 1201 2079
Size: 202 233 766 878
Fragments arranged by size:
878 766 233 202
BspHI T'CATG_A
Cuts at: 0 1341 2079
Size: 1341 738
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 873 2079
Size: 873 1206
BsrBI CCG'CTC
Cuts at: 0 99 537 992 2079
Size: 99 438 455 1087
Fragments arranged by size:
1087 455 438 99
BsrDI GCAATG_nn'
Cuts at: 0 1770 2079
Size: 1770 309
BsrFI r'CCGG_y
Cuts at: 0 998 2079
Size: 998 1081
BsrGI T'GTAC_A
Cuts at: 0 402 2079
Size: 402 1677
BstXI CCAn_nnnn'nTGG
Cuts at: 0 794 1028 2045 2079
Size: 794 234 1017 34
Fragments arranged by size:
1017 794 234 34
BstYI r'GATC_y
Cuts at: 0 248 672 891 1316 1352 2079
Size: 248 424 219 425 36 727
Fragments arranged by size:
727 425 424 248 219 36
Bsu36I CC'TnA_GG
Cuts at: 0 362 2079
Size: 362 1717
BtgI C'CryG_G
Cuts at: 0 332 908 1653 2079
Size: 332 576 745 426
Fragments arranged by size:
745 576 426 332
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1802 2079
Size: 1802 277
BtsI GCAGTG_nn'
Cuts at: 0 458 689 1301 1755 2079
Size: 458 231 612 454 324
Fragments arranged by size:
612 458 454 324 231
ClaI AT'CG_AT
Cuts at: 0 389 2079
Size: 389 1690
EaeI y'GGCC_r
Cuts at: 0 26 173 2079
Size: 26 147 1906
Fragments arranged by size:
1906 147 26
EarI CTCTTCn'nnn_
Cuts at: 0 481 491 595 1580 1895 2056 2079
Size: 481 10 104 985 315 161 23
Fragments arranged by size:
985 481 315 161 104 23 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 591 2079
Size: 591 1488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 676 697 775 1185 1574 2014 2079
Size: 127 549 21 78 410 389 440 65
Fragments arranged by size:
549 440 410 389 127 78 65 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1120 2079
Size: 1120 959
EcoO109I rG'GnC_Cy
Cuts at: 0 19 199 431 432 1458 2034 2079
Size: 19 180 232 1 1026 576 45
Fragments arranged by size:
1026 576 232 180 45 19 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 948 2079
Size: 948 1131
HaeII r_GCGC'y
Cuts at: 0 135 325 466 2079
Size: 135 190 141 1613
Fragments arranged by size:
1613 190 141 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 755 787 2079
Size: 755 32 1292
Fragments arranged by size:
1292 755 32
HincII GTy'rAC
Cuts at: 0 480 1167 2079
Size: 480 687 912
Fragments arranged by size:
912 687 480
HpaI GTT'AAC
Cuts at: 0 1167 2079
Size: 1167 912
KasI G'GCGC_C
Cuts at: 0 321 462 2079
Size: 321 141 1617
Fragments arranged by size:
1617 321 141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 2079
Size: 129 1950
MslI CAynn'nnrTG
Cuts at: 0 511 736 958 2079
Size: 511 225 222 1121
Fragments arranged by size:
1121 511 225 222
MspA1I CmG'CkG
Cuts at: 0 114 669 1028 1097 2079
Size: 114 555 359 69 982
Fragments arranged by size:
982 555 359 114 69
NaeI GCC'GGC
Cuts at: 0 1000 2079
Size: 1000 1079
NarI GG'CG_CC
Cuts at: 0 322 463 2079
Size: 322 141 1616
Fragments arranged by size:
1616 322 141
NcoI C'CATG_G
Cuts at: 0 908 1653 2079
Size: 908 745 426
Fragments arranged by size:
908 745 426
NgoMIV G'CCGG_C
Cuts at: 0 998 2079
Size: 998 1081
PfoI T'CCnGG_A
Cuts at: 0 143 752 1201 2079
Size: 143 609 449 878
Fragments arranged by size:
878 609 449 143
PpuMI rG'GwC_Cy
Cuts at: 0 1458 2034 2079
Size: 1458 576 45
Fragments arranged by size:
1458 576 45
PspOMI G'GGCC_C
Cuts at: 0 198 431 2079
Size: 198 233 1648
Fragments arranged by size:
1648 233 198
PstI C_TGCA'G
Cuts at: 0 650 884 2079
Size: 650 234 1195
Fragments arranged by size:
1195 650 234
PvuII CAG'CTG
Cuts at: 0 669 1097 2079
Size: 669 428 982
Fragments arranged by size:
982 669 428
SapI GCTCTTCn'nnn_
Cuts at: 0 481 2079
Size: 481 1598
SbfI CC_TGCA'GG
Cuts at: 0 650 2079
Size: 650 1429
ScaI AGT'ACT
Cuts at: 0 1813 2079
Size: 1813 266
SfcI C'TryA_G
Cuts at: 0 444 646 880 2079
Size: 444 202 234 1199
Fragments arranged by size:
1199 444 234 202
SfoI GGC'GCC
Cuts at: 0 323 464 2079
Size: 323 141 1615
Fragments arranged by size:
1615 323 141
SmlI C'TyrA_G
Cuts at: 0 235 718 2079
Size: 235 483 1361
Fragments arranged by size:
1361 483 235
StuI AGG'CCT
Cuts at: 0 367 2079
Size: 367 1712
StyI C'CwwG_G
Cuts at: 0 908 1653 2079
Size: 908 745 426
Fragments arranged by size:
908 745 426
TatI w'GTAC_w
Cuts at: 0 402 929 1811 2079
Size: 402 527 882 268
Fragments arranged by size:
882 527 402 268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 339 2079
Size: 339 1740
XhoI C'TCGA_G
Cuts at: 0 235 2079
Size: 235 1844
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BclI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HpaI KasI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV PfoI PpuMI PspOMI
PstI PvuII SapI SbfI ScaI SfcI SfoI SmlI
StuI StyI TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BfrBI BglI
BlpI BmtI BsaAI BsaXI BsiEI BsiHKAI BsiWI BspEI
BssHII BssSI BstAPI BstBI BstEII BstZ17I CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspI
FspAI HindIII KpnI MfeI MluI MscI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XmaI XmnI ZraI