Restriction map for
pF1KB8692
(Linear) (Six-base) MAPSORT of: KIBB8692.seq Check: 1386 from: 1 to: 1437
KIBB8692.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AclI AA'CG_TT
Cuts at: 0 1377 1437
Size: 1377 60
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1211 1437
Size: 1147 64 226
Fragments arranged by size:
1147 226 64
AfeI AGC'GCT
Cuts at: 0 524 1437
Size: 524 913
AflII C'TTAA_G
Cuts at: 0 982 1437
Size: 982 455
AhdI GACnn_n'nnGTC
Cuts at: 0 886 1437
Size: 886 551
AlwNI CAG_nnn'CTG
Cuts at: 0 951 1437
Size: 951 486
ApoI r'AATT_y
Cuts at: 0 915 1437
Size: 915 522
AvaI C'yCGr_G
Cuts at: 0 167 434 1437
Size: 167 267 1003
Fragments arranged by size:
1003 267 167
AvrII C'CTAG_G
Cuts at: 0 1267 1437
Size: 1267 170
BamHI G'GATC_C
Cuts at: 0 369 1437
Size: 369 1068
BanI G'GyrC_C
Cuts at: 0 509 1437
Size: 509 928
BanII G_rGCy'C
Cuts at: 0 340 1437
Size: 340 1097
BbeI G_GCGC'C
Cuts at: 0 513 1437
Size: 513 924
BbsI GAAGACnn'nnnn_
Cuts at: 0 698 1154 1437
Size: 698 456 283
Fragments arranged by size:
698 456 283
BbvCI CC'TCA_GC
Cuts at: 0 356 1437
Size: 356 1081
BclI T'GATC_A
Cuts at: 0 920 1437
Size: 920 517
BglII A'GATC_T
Cuts at: 0 1086 1437
Size: 1086 351
BmrI ACTGGGnnnn_n'
Cuts at: 0 368 1437
Size: 368 1069
BmtI G_CTAG'C
Cuts at: 0 250 1437
Size: 250 1187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1437
Size: 751 686
Bpu10I CC'TnA_GC
Cuts at: 0 177 182 356 1437
Size: 177 5 174 1081
Fragments arranged by size:
1081 177 174 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1437
Size: 813 624
BsaBI GATnn'nnATC
Cuts at: 0 1289 1437
Size: 1289 148
BsaHI Gr'CG_yC
Cuts at: 0 510 1437
Size: 510 927
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 724 754 1437
Size: 724 30 683
Fragments arranged by size:
724 683 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 241 244 247 250 253 313 316
Size: 229 12 3 3 3 3 60 3
Cuts at: 316 319 322 325 478 481 493 496 499
Size: 3 3 3 153 3 12 3 3
Cuts at: 499 502 1437
Size: 3 935
Fragments arranged by size:
935 229 153 60 12 12 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 870 1437
Size: 870 567
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1427 1437
Size: 1427 10
BsiHKAI G_wGCw'C
Cuts at: 0 340 1437
Size: 340 1097
BsmI GAATG_Cn'
Cuts at: 0 294 321 1400 1437
Size: 294 27 1079 37
Fragments arranged by size:
1079 294 37 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 203 1437
Size: 203 1234
Bsp1286I G_dGCh'C
Cuts at: 0 340 1437
Size: 340 1097
BspHI T'CATG_A
Cuts at: 0 153 1437
Size: 153 1284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 1437
Size: 49 1388
BsrFI r'CCGG_y
Cuts at: 0 440 1437
Size: 440 997
BstYI r'GATC_y
Cuts at: 0 10 369 1086 1437
Size: 10 359 717 351
Fragments arranged by size:
717 359 351 10
Bsu36I CC'TnA_GG
Cuts at: 0 581 1437
Size: 581 856
DrdI GACnn_nn'nnGTC
Cuts at: 0 700 1437
Size: 700 737
EarI CTCTTCn'nnn_
Cuts at: 0 1317 1335 1364 1437
Size: 1317 18 29 73
Fragments arranged by size:
1317 73 29 18
EcoICRI GAG'CTC
Cuts at: 0 338 1437
Size: 338 1099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1147 1211 1437
Size: 751 396 64 226
Fragments arranged by size:
751 396 226 64
EcoO109I rG'GnC_Cy
Cuts at: 0 61 1437
Size: 61 1376
HaeII r_GCGC'y
Cuts at: 0 513 526 1437
Size: 513 13 911
Fragments arranged by size:
911 513 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 872 904 1437
Size: 148 32 692 32 533
Fragments arranged by size:
692 533 148 32 32
HincII GTy'rAC
Cuts at: 0 451 710 977 1437
Size: 451 259 267 460
Fragments arranged by size:
460 451 267 259
HindIII A'AGCT_T
Cuts at: 0 1429 1437
Size: 1429 8
KasI G'GCGC_C
Cuts at: 0 509 1437
Size: 509 928
MspA1I CmG'CkG
Cuts at: 0 43 507 1437
Size: 43 464 930
Fragments arranged by size:
930 464 43
NaeI GCC'GGC
Cuts at: 0 442 1437
Size: 442 995
NarI GG'CG_CC
Cuts at: 0 510 1437
Size: 510 927
NgoMIV G'CCGG_C
Cuts at: 0 440 1437
Size: 440 997
NheI G'CTAG_C
Cuts at: 0 246 1437
Size: 246 1191
PflMI CCAn_nnn'nTGG
Cuts at: 0 877 1437
Size: 877 560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1334 1366 1437
Size: 1334 32 71
Fragments arranged by size:
1334 71 32
PpuMI rG'GwC_Cy
Cuts at: 0 61 1437
Size: 61 1376
PshAI GACnn'nnGTC
Cuts at: 0 604 1437
Size: 604 833
PvuII CAG'CTG
Cuts at: 0 43 1437
Size: 43 1394
SacI G_AGCT'C
Cuts at: 0 340 1437
Size: 340 1097
SapI GCTCTTCn'nnn_
Cuts at: 0 1317 1364 1437
Size: 1317 47 73
Fragments arranged by size:
1317 73 47
ScaI AGT'ACT
Cuts at: 0 1132 1437
Size: 1132 305
SfoI GGC'GCC
Cuts at: 0 511 1437
Size: 511 926
SmaI CCC'GGG
Cuts at: 0 169 1437
Size: 169 1268
SmlI C'TyrA_G
Cuts at: 0 792 982 1437
Size: 792 190 455
Fragments arranged by size:
792 455 190
StuI AGG'CCT
Cuts at: 0 361 562 1437
Size: 361 201 875
Fragments arranged by size:
875 361 201
StyI C'CwwG_G
Cuts at: 0 53 1267 1437
Size: 53 1214 170
Fragments arranged by size:
1214 170 53
TatI w'GTAC_w
Cuts at: 0 198 803 1130 1437
Size: 198 605 327 307
Fragments arranged by size:
605 327 307 198
XmaI C'CCGG_G
Cuts at: 0 167 1437
Size: 167 1270
XmnI GAAnn'nnTTC
Cuts at: 0 1010 1437
Size: 1010 427
Enzymes that do cut:
AclI AcuI AfeI AflII AhdI AlwNI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BclI
BglII BmrI BmtI BpmI Bpu10I BpuEI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrFI BstYI Bsu36I DrdI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII HindIII KasI MspA1I
NaeI NarI NgoMIV NheI PflMI PpiI PpuMI PshAI
PvuII SacI SapI ScaI SfoI SmaI SmlI StuI
StyI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflIII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI BaeI BcgI
BciVI BfrBI BglI BlpI Bme1580I BmgBI BplI BsaI
BsaAI BsaWI BsiEI BsiWI BspEI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI ZraI