Restriction map for
pF1KB8693
(Linear) (Six-base) MAPSORT of: KIBB8693.seq Check: 9867 from: 1 to: 1614
KIBB8693.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AatII G_ACGT'C
Cuts at: 0 530 1614
Size: 530 1084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 952 1093 1243 1614
Size: 38 914 141 150 371
Fragments arranged by size:
914 371 150 141 38
AfeI AGC'GCT
Cuts at: 0 712 1614
Size: 712 902
AflIII A'CryG_T
Cuts at: 0 305 1064 1130 1614
Size: 305 759 66 484
Fragments arranged by size:
759 484 305 66
AhdI GACnn_n'nnGTC
Cuts at: 0 385 1614
Size: 385 1229
AlwNI CAG_nnn'CTG
Cuts at: 0 78 1614
Size: 78 1536
ApoI r'AATT_y
Cuts at: 0 757 1614
Size: 757 857
AvaI C'yCGr_G
Cuts at: 0 11 1253 1614
Size: 11 1242 361
Fragments arranged by size:
1242 361 11
BanI G'GyrC_C
Cuts at: 0 1543 1614
Size: 1543 71
BanII G_rGCy'C
Cuts at: 0 803 1058 1614
Size: 803 255 556
Fragments arranged by size:
803 556 255
BbsI GAAGACnn'nnnn_
Cuts at: 0 1231 1283 1614
Size: 1231 52 331
Fragments arranged by size:
1231 331 52
BciVI GTATCCnnnnn_n'
Cuts at: 0 755 1614
Size: 755 859
BglI GCCn_nnn'nGGC
Cuts at: 0 439 1489 1614
Size: 439 1050 125
Fragments arranged by size:
1050 439 125
BlpI GC'TnA_GC
Cuts at: 0 1147 1479 1614
Size: 1147 332 135
Fragments arranged by size:
1147 332 135
Bme1580I G_kGCm'C
Cuts at: 0 1130 1614
Size: 1130 484
BmgBI CAC'GTC
Cuts at: 0 1067 1614
Size: 1067 547
BmrI ACTGGGnnnn_n'
Cuts at: 0 388 1379 1526 1614
Size: 388 991 147 88
Fragments arranged by size:
991 388 147 88
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 1230 1494 1614
Size: 123 1107 264 120
Fragments arranged by size:
1107 264 123 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 205 295 903 1582 1614
Size: 118 87 90 608 679 32
Fragments arranged by size:
679 608 118 90 87 32
Bpu10I CC'TnA_GC
Cuts at: 0 468 1614
Size: 468 1146
BsaHI Gr'CG_yC
Cuts at: 0 527 1614
Size: 527 1087
BsaWI w'CCGG_w
Cuts at: 0 1540 1589 1614
Size: 1540 49 25
Fragments arranged by size:
1540 49 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1227 1257 1614
Size: 1227 30 357
Fragments arranged by size:
1227 357 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 550 675 721 1174 1222 1248 1614
Size: 115 435 125 46 453 48 26 366
Fragments arranged by size:
453 435 366 125 115 48 46 26
BseYI C'CCAG_C
Cuts at: 0 78 707 998 1404 1614
Size: 78 629 291 406 210
Fragments arranged by size:
629 406 291 210 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 518 1614
Size: 393 125 1096
Fragments arranged by size:
1096 393 125
BsiHKAI G_wGCw'C
Cuts at: 0 549 1614
Size: 549 1065
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1287 1614
Size: 1287 327
Bsp1286I G_dGCh'C
Cuts at: 0 549 803 1058 1130 1614
Size: 549 254 255 72 484
Fragments arranged by size:
549 484 255 254 72
BspEI T'CCGG_A
Cuts at: 0 1589 1614
Size: 1589 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 416 1614
Size: 416 1198
BsrDI GCAATG_nn'
Cuts at: 0 1611 1614
Size: 1611 3
BsrFI r'CCGG_y
Cuts at: 0 4 145 1614
Size: 4 141 1469
Fragments arranged by size:
1469 141 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 505 1473 1614
Size: 505 968 141
Fragments arranged by size:
968 505 141
BstBI TT'CG_AA
Cuts at: 0 552 1614
Size: 552 1062
BstYI r'GATC_y
Cuts at: 0 903 1085 1614
Size: 903 182 529
Fragments arranged by size:
903 529 182
Bsu36I CC'TnA_GG
Cuts at: 0 71 1614
Size: 71 1543
BtgI C'CryG_G
Cuts at: 0 1305 1391 1575 1614
Size: 1305 86 184 39
Fragments arranged by size:
1305 184 86 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1305 1340 1614
Size: 1305 35 274
Fragments arranged by size:
1305 274 35
DraIII CAC_nnn'GTG
Cuts at: 0 267 1614
Size: 267 1347
EaeI y'GGCC_r
Cuts at: 0 2 195 246 431 1614
Size: 2 193 51 185 1183
Fragments arranged by size:
1183 193 185 51 2
EarI CTCTTCn'nnn_
Cuts at: 0 57 169 554 1021 1126 1234 1614
Size: 57 112 385 467 105 108 380
Fragments arranged by size:
467 385 380 112 108 105 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 118 205 295 903 952 1093 1243
Size: 38 80 87 90 608 49 141 150
Cuts at: 1243 1582 1614
Size: 339 32
Fragments arranged by size:
608 339 150 141 90 87 80 49
38 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 1614
Size: 406 1208
EcoO109I rG'GnC_Cy
Cuts at: 0 41 1554 1614
Size: 41 1513 60
Fragments arranged by size:
1513 60 41
EcoRV GAT'ATC
Cuts at: 0 240 1614
Size: 240 1374
HaeII r_GCGC'y
Cuts at: 0 11 714 1417 1614
Size: 11 703 703 197
Fragments arranged by size:
703 703 197 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 852 876 884 908 1614
Size: 371 32 449 24 8 24 706
Fragments arranged by size:
706 449 371 32 24 24 8
HincII GTy'rAC
Cuts at: 0 954 1614
Size: 954 660
HpaI GTT'AAC
Cuts at: 0 954 1614
Size: 954 660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1563 1614
Size: 1563 51
MscI TGG'CCA
Cuts at: 0 248 1614
Size: 248 1366
MslI CAynn'nnrTG
Cuts at: 0 1285 1614
Size: 1285 329
MspA1I CmG'CkG
Cuts at: 0 28 140 971 1614
Size: 28 112 831 643
Fragments arranged by size:
831 643 112 28
NaeI GCC'GGC
Cuts at: 0 6 1614
Size: 6 1608
NgoMIV G'CCGG_C
Cuts at: 0 4 1614
Size: 4 1610
NspI r_CATG'y
Cuts at: 0 1134 1290 1614
Size: 1134 156 324
Fragments arranged by size:
1134 324 156
PciI A'CATG_T
Cuts at: 0 1130 1614
Size: 1130 484
PfoI T'CCnGG_A
Cuts at: 0 109 272 1614
Size: 109 163 1342
Fragments arranged by size:
1342 163 109
PpuMI rG'GwC_Cy
Cuts at: 0 41 1614
Size: 41 1573
PstI C_TGCA'G
Cuts at: 0 1469 1614
Size: 1469 145
SapI GCTCTTCn'nnn_
Cuts at: 0 554 1614
Size: 554 1060
SfcI C'TryA_G
Cuts at: 0 1465 1614
Size: 1465 149
SmaI CCC'GGG
Cuts at: 0 1255 1614
Size: 1255 359
SphI G_CATG'C
Cuts at: 0 1290 1614
Size: 1290 324
StyI C'CwwG_G
Cuts at: 0 128 1323 1614
Size: 128 1195 291
Fragments arranged by size:
1195 291 128
XmaI C'CCGG_G
Cuts at: 0 1253 1614
Size: 1253 361
ZraI GAC'GTC
Cuts at: 0 528 1614
Size: 528 1086
Enzymes that do cut:
AatII AcuI AfeI AflIII AhdI AlwNI ApoI AvaI
BanI BanII BbsI BciVI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BstAPI BstBI BstYI Bsu36I BtgI CspCI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HincII HpaI MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PciI PfoI PpuMI PstI SapI SfcI SmaI
SphI StyI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BfrBI BglII BmtI
BpuEI BsaI BsaAI BsaBI BsiEI BsiWI BsmI BspHI
BsrBI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI
BtsI ClaI DraI DrdI EagI EciI EcoICRI EcoRI
FalI FseI FspI FspAI HindIII KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI