Restriction map for
pF1KB8694
(Linear) (Six-base) MAPSORT of: KIBB8694.seq Check: 4675 from: 1 to: 1365
KIBB8694.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 0 453 1365
Size: 453 912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 679 1232 1365
Size: 628 51 553 133
Fragments arranged by size:
628 553 133 51
AflIII A'CryG_T
Cuts at: 0 1203 1365
Size: 1203 162
AlwNI CAG_nnn'CTG
Cuts at: 0 208 815 1365
Size: 208 607 550
Fragments arranged by size:
607 550 208
ApoI r'AATT_y
Cuts at: 0 742 1365
Size: 742 623
AvaI C'yCGr_G
Cuts at: 0 10 302 587 1365
Size: 10 292 285 778
Fragments arranged by size:
778 292 285 10
AvrII C'CTAG_G
Cuts at: 0 906 1365
Size: 906 459
BamHI G'GATC_C
Cuts at: 0 910 1365
Size: 910 455
BanI G'GyrC_C
Cuts at: 0 6 226 1365
Size: 6 220 1139
Fragments arranged by size:
1139 220 6
BanII G_rGCy'C
Cuts at: 0 501 1365
Size: 501 864
BbeI G_GCGC'C
Cuts at: 0 230 1365
Size: 230 1135
BciVI GTATCCnnnnn_n'
Cuts at: 0 744 1365
Size: 744 621
BglII A'GATC_T
Cuts at: 0 320 1365
Size: 320 1045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1304 1355 1365
Size: 280 1024 51 10
Fragments arranged by size:
1024 280 51 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 1365
Size: 513 852
BsaI GGTCTCn'nnnn_
Cuts at: 0 256 1365
Size: 256 1109
BsaHI Gr'CG_yC
Cuts at: 0 227 1365
Size: 227 1138
BsaWI w'CCGG_w
Cuts at: 0 1078 1365
Size: 1078 287
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 1365
Size: 73 1292
BseYI C'CCAG_C
Cuts at: 0 41 1069 1365
Size: 41 1028 296
Fragments arranged by size:
1028 296 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1365
Size: 382 983
BsiEI CG_ry'CG
Cuts at: 0 32 531 1365
Size: 32 499 834
Fragments arranged by size:
834 499 32
BsiHKAI G_wGCw'C
Cuts at: 0 501 1365
Size: 501 864
Bsp1286I G_dGCh'C
Cuts at: 0 501 1365
Size: 501 864
BspEI T'CCGG_A
Cuts at: 0 1078 1365
Size: 1078 287
BstYI r'GATC_y
Cuts at: 0 320 569 910 1365
Size: 320 249 341 455
Fragments arranged by size:
455 341 320 249
BtgI C'CryG_G
Cuts at: 0 879 1365
Size: 879 486
BtsI GCAGTG_nn'
Cuts at: 0 253 863 1365
Size: 253 610 502
Fragments arranged by size:
610 502 253
DraI TTT'AAA
Cuts at: 0 78 1365
Size: 78 1287
DrdI GACnn_nn'nnGTC
Cuts at: 0 36 890 1365
Size: 36 854 475
Fragments arranged by size:
854 475 36
EarI CTCTTCn'nnn_
Cuts at: 0 50 613 1126 1365
Size: 50 563 513 239
Fragments arranged by size:
563 513 239 50
EcoICRI GAG'CTC
Cuts at: 0 499 1365
Size: 499 866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 628 679 1232 1304 1355 1365
Size: 280 348 51 553 72 51 10
Fragments arranged by size:
553 348 280 72 51 51 10
EcoO109I rG'GnC_Cy
Cuts at: 0 347 1365
Size: 347 1018
EcoRV GAT'ATC
Cuts at: 0 189 1365
Size: 189 1176
HaeII r_GCGC'y
Cuts at: 0 230 906 1077 1365
Size: 230 676 171 288
Fragments arranged by size:
676 288 230 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 1365
Size: 382 32 951
Fragments arranged by size:
951 382 32
HincII GTy'rAC
Cuts at: 0 454 564 1008 1365
Size: 454 110 444 357
Fragments arranged by size:
454 444 357 110
HindIII A'AGCT_T
Cuts at: 0 110 1365
Size: 110 1255
KasI G'GCGC_C
Cuts at: 0 226 1365
Size: 226 1139
MluI A'CGCG_T
Cuts at: 0 1203 1365
Size: 1203 162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1192 1365
Size: 52 1140 173
Fragments arranged by size:
1140 173 52
MslI CAynn'nnrTG
Cuts at: 0 995 1365
Size: 995 370
NarI GG'CG_CC
Cuts at: 0 227 1365
Size: 227 1138
NcoI C'CATG_G
Cuts at: 0 879 1365
Size: 879 486
NruI TCG'CGA
Cuts at: 0 1272 1365
Size: 1272 93
PasI CC'CwG_GG
Cuts at: 0 342 1365
Size: 342 1023
PpuMI rG'GwC_Cy
Cuts at: 0 347 1365
Size: 347 1018
PstI C_TGCA'G
Cuts at: 0 811 1365
Size: 811 554
SacI G_AGCT'C
Cuts at: 0 501 1365
Size: 501 864
SalI G'TCGA_C
Cuts at: 0 452 1365
Size: 452 913
SexAI A'CCwGG_T
Cuts at: 0 758 1158 1365
Size: 758 400 207
Fragments arranged by size:
758 400 207
SfcI C'TryA_G
Cuts at: 0 807 1365
Size: 807 558
SfoI GGC'GCC
Cuts at: 0 228 1365
Size: 228 1137
SmlI C'TyrA_G
Cuts at: 0 492 587 1365
Size: 492 95 778
Fragments arranged by size:
778 492 95
SspI AAT'ATT
Cuts at: 0 721 1365
Size: 721 644
StyI C'CwwG_G
Cuts at: 0 879 906 932 1031 1365
Size: 879 27 26 99 334
Fragments arranged by size:
879 334 99 27 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 76 545 1365
Size: 46 30 469 820
Fragments arranged by size:
820 469 46 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 975 1365
Size: 975 390
XhoI C'TCGA_G
Cuts at: 0 587 1365
Size: 587 778
XmnI GAAnn'nnTTC
Cuts at: 0 161 390 484 601 1365
Size: 161 229 94 117 764
Fragments arranged by size:
764 229 161 117 94
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI AvaI AvrII BamHI
BanI BanII BbeI BciVI BglII BpmI BpuEI BsaI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BstYI BtgI BtsI DraI DrdI EarI EcoICRI
Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII KasI
MluI MmeI MslI NarI NcoI NruI PasI PpuMI
PstI SacI SalI SexAI SfcI SfoI SmlI SspI
StyI TaqII XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BbsI BbvCI BcgI BclI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI
BsaXI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI EagI
EciI EcoNI EcoRI FalI FseI FspI FspAI HpaI
KpnI MfeI MscI MspA1I NaeI NdeI NgoMIV NheI
NotI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TatI TstI Tth111I XbaI XmaI ZraI