Restriction map for
pF1KB8695
(Linear) (Six-base) MAPSORT of: KIBB8695.seq Check: 7440 from: 1 to: 2160
KIBB8695.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 135 2160
Size: 135 2025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1202 1243 2160
Size: 1027 175 41 917
Fragments arranged by size:
1027 917 175 41
AflII C'TTAA_G
Cuts at: 0 1621 2160
Size: 1621 539
AflIII A'CryG_T
Cuts at: 0 1672 2160
Size: 1672 488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 213 2160
Size: 213 1947
AlwNI CAG_nnn'CTG
Cuts at: 0 751 784 2160
Size: 751 33 1376
Fragments arranged by size:
1376 751 33
ApoI r'AATT_y
Cuts at: 0 401 595 1174 1277 1328 2160
Size: 401 194 579 103 51 832
Fragments arranged by size:
832 579 401 194 103 51
AseI AT'TA_AT
Cuts at: 0 8 632 2099 2160
Size: 8 624 1467 61
Fragments arranged by size:
1467 624 61 8
AvaI C'yCGr_G
Cuts at: 0 1088 2160
Size: 1088 1072
BamHI G'GATC_C
Cuts at: 0 1302 2160
Size: 1302 858
BanI G'GyrC_C
Cuts at: 0 1453 2160
Size: 1453 707
BciVI GTATCCnnnnn_n'
Cuts at: 0 1791 2160
Size: 1791 369
BclI T'GATC_A
Cuts at: 0 990 2160
Size: 990 1170
BfrBI ATG'CAT
Cuts at: 0 1588 1734 2160
Size: 1588 146 426
Fragments arranged by size:
1588 426 146
BglII A'GATC_T
Cuts at: 0 739 2160
Size: 739 1421
Bme1580I G_kGCm'C
Cuts at: 0 1792 2160
Size: 1792 368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 728 1357 2160
Size: 135 593 629 803
Fragments arranged by size:
803 629 593 135
Bpu10I CC'TnA_GC
Cuts at: 0 104 2160
Size: 104 2056
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 252 282 1184 1214 2160
Size: 252 30 902 30 946
Fragments arranged by size:
946 902 252 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1036 2160
Size: 1036 1124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 2160
Size: 1625 535
Bsp1286I G_dGCh'C
Cuts at: 0 1792 2160
Size: 1792 368
BsrGI T'GTAC_A
Cuts at: 0 786 891 2160
Size: 786 105 1269
Fragments arranged by size:
1269 786 105
BstYI r'GATC_y
Cuts at: 0 739 747 1302 1636 2160
Size: 739 8 555 334 524
Fragments arranged by size:
739 555 524 334 8
Bsu36I CC'TnA_GG
Cuts at: 0 1679 2160
Size: 1679 481
BtgI C'CryG_G
Cuts at: 0 228 881 2160
Size: 228 653 1279
Fragments arranged by size:
1279 653 228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 579 614 1343 1378 2160
Size: 579 35 729 35 782
Fragments arranged by size:
782 729 579 35 35
EaeI y'GGCC_r
Cuts at: 0 1753 2160
Size: 1753 407
EarI CTCTTCn'nnn_
Cuts at: 0 509 719 968 1046 1262 2160
Size: 509 210 249 78 216 898
Fragments arranged by size:
898 509 249 216 210 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 728 1027 1202 1243 1357 2160
Size: 135 593 299 175 41 114 803
Fragments arranged by size:
803 593 299 175 135 114 41
EcoO109I rG'GnC_Cy
Cuts at: 0 1085 2160
Size: 1085 1075
EcoRI G'AATT_C
Cuts at: 0 1328 2160
Size: 1328 832
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1149 1181 1184 1216 2160
Size: 1149 32 3 32 944
Fragments arranged by size:
1149 944 32 32 3
HindIII A'AGCT_T
Cuts at: 0 896 1836 2160
Size: 896 940 324
Fragments arranged by size:
940 896 324
MfeI C'AATT_G
Cuts at: 0 1337 2160
Size: 1337 823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1539 1558 1988 2160
Size: 1539 19 430 172
Fragments arranged by size:
1539 430 172 19
MscI TGG'CCA
Cuts at: 0 1755 2160
Size: 1755 405
MslI CAynn'nnrTG
Cuts at: 0 298 2160
Size: 298 1862
MspA1I CmG'CkG
Cuts at: 0 2001 2160
Size: 2001 159
NcoI C'CATG_G
Cuts at: 0 228 2160
Size: 228 1932
NdeI CA'TA_TG
Cuts at: 0 1487 2160
Size: 1487 673
NsiI A_TGCA'T
Cuts at: 0 1590 1736 2160
Size: 1590 146 424
Fragments arranged by size:
1590 424 146
NspI r_CATG'y
Cuts at: 0 1676 2160
Size: 1676 484
PasI CC'CwG_GG
Cuts at: 0 1785 2160
Size: 1785 375
PciI A'CATG_T
Cuts at: 0 1672 2160
Size: 1672 488
PflMI CCAn_nnn'nTGG
Cuts at: 0 751 2160
Size: 751 1409
PfoI T'CCnGG_A
Cuts at: 0 689 2160
Size: 689 1471
PpuMI rG'GwC_Cy
Cuts at: 0 1085 2160
Size: 1085 1075
PsiI TTA'TAA
Cuts at: 0 767 2160
Size: 767 1393
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 79 111 2160
Size: 79 32 2049
Fragments arranged by size:
2049 79 32
PstI C_TGCA'G
Cuts at: 0 1269 2006 2093 2160
Size: 1269 737 87 67
Fragments arranged by size:
1269 737 87 67
PvuII CAG'CTG
Cuts at: 0 2001 2160
Size: 2001 159
SapI GCTCTTCn'nnn_
Cuts at: 0 968 2160
Size: 968 1192
ScaI AGT'ACT
Cuts at: 0 318 2160
Size: 318 1842
SfcI C'TryA_G
Cuts at: 0 17 1265 1874 2002 2089 2160
Size: 17 1248 609 128 87 71
Fragments arranged by size:
1248 609 128 87 71 17
SmaI CCC'GGG
Cuts at: 0 1090 2160
Size: 1090 1070
SmlI C'TyrA_G
Cuts at: 0 1621 2160
Size: 1621 539
SspI AAT'ATT
Cuts at: 0 2066 2160
Size: 2066 94
StyI C'CwwG_G
Cuts at: 0 228 272 1493 2160
Size: 228 44 1221 667
Fragments arranged by size:
1221 667 228 44
TatI w'GTAC_w
Cuts at: 0 316 786 891 2160
Size: 316 470 105 1269
Fragments arranged by size:
1269 470 316 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 168 200 252 284 1178 1210 2160
Size: 168 32 52 32 894 32 950
Fragments arranged by size:
950 894 168 52 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1170 1968 2160
Size: 1170 798 192
Fragments arranged by size:
1170 798 192
XmaI C'CCGG_G
Cuts at: 0 1088 2160
Size: 1088 1072
XmnI GAAnn'nnTTC
Cuts at: 0 1256 1616 2138 2160
Size: 1256 360 522 22
Fragments arranged by size:
1256 522 360 22
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AlwNI ApoI AseI
AvaI BamHI BanI BciVI BclI BfrBI BglII Bme1580I
BpmI Bpu10I BsaXI BseRI BsgI Bsp1286I BsrGI BstYI
Bsu36I BtgI CspCI EaeI EarI Eco57MI EcoO109I EcoRI
Hin4I HindIII MfeI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PciI PflMI PfoI PpuMI
PsiI PsrI PstI PvuII SapI ScaI SfcI SmaI
SmlI SspI StyI TatI TstI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BanII
BbeI BbsI BbvCI BcgI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TaqII
Tth111I XcmI XhoI ZraI