Restriction map for
pF1KB8697
(Linear) (Six-base) MAPSORT of: KIBB8697.seq Check: 9494 from: 1 to: 1638
KIBB8697.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 686 1638
Size: 686 952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1638
Size: 1168 470
AgeI A'CCGG_T
Cuts at: 0 1295 1638
Size: 1295 343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1175 1638
Size: 1175 463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 1638
Size: 1186 32 420
Fragments arranged by size:
1186 420 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1005 1461 1638
Size: 1005 456 177
Fragments arranged by size:
1005 456 177
ApaI G_GGCC'C
Cuts at: 0 201 283 789 1638
Size: 201 82 506 849
Fragments arranged by size:
849 506 201 82
ApoI r'AATT_y
Cuts at: 0 612 1638
Size: 612 1026
AvaI C'yCGr_G
Cuts at: 0 79 788 1638
Size: 79 709 850
Fragments arranged by size:
850 709 79
BamHI G'GATC_C
Cuts at: 0 414 1638
Size: 414 1224
BanI G'GyrC_C
Cuts at: 0 1061 1638
Size: 1061 577
BanII G_rGCy'C
Cuts at: 0 90 174 201 283 789 1483 1530 1622
Size: 90 84 27 82 506 694 47 92
Cuts at: 1622 1638
Size: 16
Fragments arranged by size:
694 506 92 90 84 82 47 27
16
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 969 1638
Size: 245 724 669
Fragments arranged by size:
724 669 245
BbvCI CC'TCA_GC
Cuts at: 0 1340 1638
Size: 1340 298
BglI GCCn_nnn'nGGC
Cuts at: 0 184 706 1518 1638
Size: 184 522 812 120
Fragments arranged by size:
812 522 184 120
BlpI GC'TnA_GC
Cuts at: 0 223 1638
Size: 223 1415
Bme1580I G_kGCm'C
Cuts at: 0 201 283 300 789 1419 1638
Size: 201 82 17 489 630 219
Fragments arranged by size:
630 489 219 201 82 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 1616 1638
Size: 1616 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 837 900 1638
Size: 837 63 738
Fragments arranged by size:
837 738 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 619 1006 1294 1390 1420 1638
Size: 362 257 387 288 96 30 218
Fragments arranged by size:
387 362 288 257 218 96 30
Bpu10I CC'TnA_GC
Cuts at: 0 1340 1638
Size: 1340 298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 742 748 910 1351 1514 1598 1638
Size: 158 584 6 162 441 163 84 40
Fragments arranged by size:
584 441 163 162 158 84 40 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 449 455 1132 1638
Size: 449 6 677 506
Fragments arranged by size:
677 506 449 6
BsaWI w'CCGG_w
Cuts at: 0 66 608 1295 1638
Size: 66 542 687 343
Fragments arranged by size:
687 542 343 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 742 772 1393 1423 1638
Size: 502 30 210 30 621 30 215
Fragments arranged by size:
621 502 215 210 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 143 232 571 616 808 811 814 973
Size: 143 89 339 45 192 3 3 159
Cuts at: 973 1198 1357 1521 1638
Size: 225 159 164 117
Fragments arranged by size:
339 225 192 164 159 159 143 117
89 45 3 3
BseYI C'CCAG_C
Cuts at: 0 100 1537 1638
Size: 100 1437 101
Fragments arranged by size:
1437 101 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1638
Size: 799 839
BsiHKAI G_wGCw'C
Cuts at: 0 253 608 692 1581 1588 1638
Size: 253 355 84 889 7 50
Fragments arranged by size:
889 355 253 84 50 7
Bsp1286I G_dGCh'C
Cuts at: 0 90 174 201 253 283 300 608 692
Size: 90 84 27 52 30 17 308 84
Cuts at: 692 789 1419 1483 1530 1581 1588 1622 1638
Size: 97 630 64 47 51 7 34 16
Fragments arranged by size:
630 308 97 90 84 84 64 52
51 47 34 30 27 17 16 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 686 1371 1638
Size: 686 685 267
Fragments arranged by size:
686 685 267
BsrBI CCG'CTC
Cuts at: 0 96 758 1059 1638
Size: 96 662 301 579
Fragments arranged by size:
662 579 301 96
BsrDI GCAATG_nn'
Cuts at: 0 1440 1638
Size: 1440 198
BsrFI r'CCGG_y
Cuts at: 0 309 1295 1638
Size: 309 986 343
Fragments arranged by size:
986 343 309
BstEII G'GTnAC_C
Cuts at: 0 816 1638
Size: 816 822
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 1638
Size: 501 1137
BstYI r'GATC_y
Cuts at: 0 414 431 1638
Size: 414 17 1207
Fragments arranged by size:
1207 414 17
BtsI GCAGTG_nn'
Cuts at: 0 1417 1638
Size: 1417 221
DraIII CAC_nnn'GTG
Cuts at: 0 304 1638
Size: 304 1334
EaeI y'GGCC_r
Cuts at: 0 1473 1638
Size: 1473 165
EarI CTCTTCn'nnn_
Cuts at: 0 422 1049 1638
Size: 422 627 589
Fragments arranged by size:
627 589 422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1617 1638
Size: 1617 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 619 1006 1168 1294 1390 1420 1638
Size: 362 257 387 162 126 96 30 218
Fragments arranged by size:
387 362 257 218 162 126 96 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1090 1629 1638
Size: 1090 539 9
Fragments arranged by size:
1090 539 9
EcoO109I rG'GnC_Cy
Cuts at: 0 76 133 197 198 279 280 990 1323
Size: 76 57 64 1 81 1 710 333
Cuts at: 1323 1374 1458 1519 1638
Size: 51 84 61 119
Fragments arranged by size:
710 333 119 84 81 76 64 61
57 51 1 1
EcoRI G'AATT_C
Cuts at: 0 612 1638
Size: 612 1026
HaeII r_GCGC'y
Cuts at: 0 1564 1638
Size: 1564 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1596 1628 1638
Size: 1596 32 10
Fragments arranged by size:
1596 32 10
HindIII A'AGCT_T
Cuts at: 0 718 1638
Size: 718 920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1416 1638
Size: 432 984 222
Fragments arranged by size:
984 432 222
MscI TGG'CCA
Cuts at: 0 1475 1638
Size: 1475 163
MspA1I CmG'CkG
Cuts at: 0 657 747 783 855 1005 1068 1638
Size: 657 90 36 72 150 63 570
Fragments arranged by size:
657 570 150 90 72 63 36
NaeI GCC'GGC
Cuts at: 0 311 1638
Size: 311 1327
NgoMIV G'CCGG_C
Cuts at: 0 309 1638
Size: 309 1329
PasI CC'CwG_GG
Cuts at: 0 1547 1638
Size: 1547 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 1398 1638
Size: 1398 240
PfoI T'CCnGG_A
Cuts at: 0 337 1355 1638
Size: 337 1018 283
Fragments arranged by size:
1018 337 283
PpuMI rG'GwC_Cy
Cuts at: 0 76 133 990 1458 1638
Size: 76 57 857 468 180
Fragments arranged by size:
857 468 180 76 57
PspOMI G'GGCC_C
Cuts at: 0 197 279 785 1638
Size: 197 82 506 853
Fragments arranged by size:
853 506 197 82
PstI C_TGCA'G
Cuts at: 0 120 746 860 1382 1424 1638
Size: 120 626 114 522 42 214
Fragments arranged by size:
626 522 214 120 114 42
PvuII CAG'CTG
Cuts at: 0 657 855 1005 1638
Size: 657 198 150 633
Fragments arranged by size:
657 633 198 150
SapI GCTCTTCn'nnn_
Cuts at: 0 1049 1638
Size: 1049 589
SbfI CC_TGCA'GG
Cuts at: 0 1382 1638
Size: 1382 256
SfcI C'TryA_G
Cuts at: 0 116 637 742 856 1378 1420 1638
Size: 116 521 105 114 522 42 218
Fragments arranged by size:
522 521 218 116 114 105 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 706 1518 1638
Size: 706 812 120
Fragments arranged by size:
812 706 120
SmaI CCC'GGG
Cuts at: 0 790 1638
Size: 790 848
SmlI C'TyrA_G
Cuts at: 0 173 721 763 925 1330 1529 1613 1638
Size: 173 548 42 162 405 199 84 25
Fragments arranged by size:
548 405 199 173 162 84 42 25
StyI C'CwwG_G
Cuts at: 0 407 494 1638
Size: 407 87 1144
Fragments arranged by size:
1144 407 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 473 1551 1638
Size: 473 1078 87
Fragments arranged by size:
1078 473 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 742 774 1393 1425 1638
Size: 742 32 619 32 213
Fragments arranged by size:
742 619 213 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 448 973 1638
Size: 448 525 665
Fragments arranged by size:
665 525 448
XmaI C'CCGG_G
Cuts at: 0 788 1638
Size: 788 850
Enzymes that do cut:
AarI AcuI AgeI AlfI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstEII BstXI BstYI BtsI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HindIII MmeI MscI MspA1I NaeI NgoMIV PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SapI SbfI SfcI
SfiI SmaI SmlI StyI TaqII TstI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MslI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI XbaI
XcmI XhoI XmnI ZraI