Restriction map for
pF1KB8698
(Linear) (Six-base) MAPSORT of: KIBB8698.seq Check: 5618 from: 1 to: 1755
KIBB8698.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
Acc65I G'GTAC_C
Cuts at: 0 1611 1755
Size: 1611 144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 1377 1755
Size: 1258 119 378
Fragments arranged by size:
1258 378 119
AflII C'TTAA_G
Cuts at: 0 114 1755
Size: 114 1641
AflIII A'CryG_T
Cuts at: 0 412 1248 1755
Size: 412 836 507
Fragments arranged by size:
836 507 412
AgeI A'CCGG_T
Cuts at: 0 1581 1755
Size: 1581 174
AleI CACnn'nnGTG
Cuts at: 0 13 1755
Size: 13 1742
AlwNI CAG_nnn'CTG
Cuts at: 0 1047 1755
Size: 1047 708
ApoI r'AATT_y
Cuts at: 0 1498 1755
Size: 1498 257
AseI AT'TA_AT
Cuts at: 0 919 1755
Size: 919 836
BanI G'GyrC_C
Cuts at: 0 540 1611 1755
Size: 540 1071 144
Fragments arranged by size:
1071 540 144
BbvCI CC'TCA_GC
Cuts at: 0 911 1001 1755
Size: 911 90 754
Fragments arranged by size:
911 754 90
BlpI GC'TnA_GC
Cuts at: 0 1628 1755
Size: 1628 127
Bme1580I G_kGCm'C
Cuts at: 0 677 1755
Size: 677 1078
BmgBI CAC'GTC
Cuts at: 0 1249 1755
Size: 1249 506
BmrI ACTGGGnnnn_n'
Cuts at: 0 673 1755
Size: 673 1082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1755
Size: 257 1498
Bpu10I CC'TnA_GC
Cuts at: 0 911 1001 1755
Size: 911 90 754
Fragments arranged by size:
911 754 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1589 1755
Size: 1589 166
BsaI GGTCTCn'nnnn_
Cuts at: 0 18 233 1481 1755
Size: 18 215 1248 274
Fragments arranged by size:
1248 274 215 18
BsaAI yAC'GTr
Cuts at: 0 415 1755
Size: 415 1340
BsaBI GATnn'nnATC
Cuts at: 0 1232 1755
Size: 1232 523
BsaWI w'CCGG_w
Cuts at: 0 1581 1755
Size: 1581 174
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 878 1058 1729 1755
Size: 878 180 671 26
Fragments arranged by size:
878 671 180 26
BseYI C'CCAG_C
Cuts at: 0 1013 1058 1755
Size: 1013 45 697
Fragments arranged by size:
1013 697 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1048 1755
Size: 710 338 707
Fragments arranged by size:
710 707 338
Bsp1286I G_dGCh'C
Cuts at: 0 677 1755
Size: 677 1078
BsrBI CCG'CTC
Cuts at: 0 1108 1755
Size: 1108 647
BsrDI GCAATG_nn'
Cuts at: 0 528 656 719 774 1755
Size: 528 128 63 55 981
Fragments arranged by size:
981 528 128 63 55
BsrFI r'CCGG_y
Cuts at: 0 1581 1755
Size: 1581 174
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1047 1179 1755
Size: 1047 132 576
Fragments arranged by size:
1047 576 132
BstEII G'GTnAC_C
Cuts at: 0 592 1584 1755
Size: 592 992 171
Fragments arranged by size:
992 592 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 15 710 1102 1755
Size: 15 695 392 653
Fragments arranged by size:
695 653 392 15
BstYI r'GATC_y
Cuts at: 0 159 418 1514 1755
Size: 159 259 1096 241
Fragments arranged by size:
1096 259 241 159
BtsI GCAGTG_nn'
Cuts at: 0 614 648 693 1119 1413 1755
Size: 614 34 45 426 294 342
Fragments arranged by size:
614 426 342 294 45 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 917 952 1755
Size: 917 35 803
Fragments arranged by size:
917 803 35
DraIII CAC_nnn'GTG
Cuts at: 0 1026 1755
Size: 1026 729
EarI CTCTTCn'nnn_
Cuts at: 0 1396 1706 1755
Size: 1396 310 49
Fragments arranged by size:
1396 310 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1258 1377 1755
Size: 257 1001 119 378
Fragments arranged by size:
1001 378 257 119
EcoO109I rG'GnC_Cy
Cuts at: 0 997 1755
Size: 997 758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 464 1755
Size: 464 1291
HincII GTy'rAC
Cuts at: 0 322 361 1755
Size: 322 39 1394
Fragments arranged by size:
1394 322 39
KpnI G_GTAC'C
Cuts at: 0 1615 1755
Size: 1615 140
MslI CAynn'nnrTG
Cuts at: 0 13 1418 1755
Size: 13 1405 337
Fragments arranged by size:
1405 337 13
MspA1I CmG'CkG
Cuts at: 0 235 604 1047 1755
Size: 235 369 443 708
Fragments arranged by size:
708 443 369 235
NspI r_CATG'y
Cuts at: 0 171 1755
Size: 171 1584
PflMI CCAn_nnn'nTGG
Cuts at: 0 193 355 1755
Size: 193 162 1400
Fragments arranged by size:
1400 193 162
PmlI CAC'GTG
Cuts at: 0 415 1755
Size: 415 1340
PpuMI rG'GwC_Cy
Cuts at: 0 997 1755
Size: 997 758
PsiI TTA'TAA
Cuts at: 0 1258 1755
Size: 1258 497
PstI C_TGCA'G
Cuts at: 0 609 1052 1755
Size: 609 443 703
Fragments arranged by size:
703 609 443
PvuII CAG'CTG
Cuts at: 0 235 604 1047 1755
Size: 235 369 443 708
Fragments arranged by size:
708 443 369 235
ScaI AGT'ACT
Cuts at: 0 1320 1755
Size: 1320 435
SfcI C'TryA_G
Cuts at: 0 605 620 1048 1755
Size: 605 15 428 707
Fragments arranged by size:
707 605 428 15
SmlI C'TyrA_G
Cuts at: 0 114 1604 1755
Size: 114 1490 151
Fragments arranged by size:
1490 151 114
SspI AAT'ATT
Cuts at: 0 825 1755
Size: 825 930
StyI C'CwwG_G
Cuts at: 0 210 761 1755
Size: 210 551 994
Fragments arranged by size:
994 551 210
TatI w'GTAC_w
Cuts at: 0 54 1318 1755
Size: 54 1264 437
Fragments arranged by size:
1264 437 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 1755
Size: 208 32 1515
Fragments arranged by size:
1515 208 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 768 1755
Size: 768 987
Enzymes that do cut:
Acc65I AcuI AflII AflIII AgeI AleI AlwNI ApoI
AseI BanI BbvCI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BseYI
BsgI Bsp1286I BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI BtsI CspCI DraIII EarI Eco57MI EcoO109I FalI
HincII KpnI MslI MspA1I NspI PflMI PmlI PpuMI
PsiI PstI PvuII ScaI SfcI SmlI SspI StyI
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AhdI AlfI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BmtI BplI BsaHI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI
ZraI