Restriction map for
pF1KB8699
(Linear) (Six-base) MAPSORT of: KIBB8699.seq Check: 9974 from: 1 to: 1623
KIBB8699.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
Acc65I G'GTAC_C
Cuts at: 0 106 1623
Size: 106 1517
AclI AA'CG_TT
Cuts at: 0 1342 1623
Size: 1342 281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 208 1623
Size: 144 64 1415
Fragments arranged by size:
1415 144 64
AflII C'TTAA_G
Cuts at: 0 598 1623
Size: 598 1025
AflIII A'CryG_T
Cuts at: 0 1012 1623
Size: 1012 611
AlwNI CAG_nnn'CTG
Cuts at: 0 1525 1623
Size: 1525 98
ApaLI G'TGCA_C
Cuts at: 0 346 1623
Size: 346 1277
ApoI r'AATT_y
Cuts at: 0 725 1206 1372 1450 1555 1623
Size: 725 481 166 78 105 68
Fragments arranged by size:
725 481 166 105 78 68
AvaI C'yCGr_G
Cuts at: 0 73 1623
Size: 73 1550
BanI G'GyrC_C
Cuts at: 0 106 220 289 708 855 1623
Size: 106 114 69 419 147 768
Fragments arranged by size:
768 419 147 114 106 69
BanII G_rGCy'C
Cuts at: 0 74 538 1623
Size: 74 464 1085
Fragments arranged by size:
1085 464 74
BbeI G_GCGC'C
Cuts at: 0 224 859 1623
Size: 224 635 764
Fragments arranged by size:
764 635 224
BbsI GAAGACnn'nnnn_
Cuts at: 0 351 1230 1604 1623
Size: 351 879 374 19
Fragments arranged by size:
879 374 351 19
BfrBI ATG'CAT
Cuts at: 0 742 1623
Size: 742 881
BglI GCCn_nnn'nGGC
Cuts at: 0 283 1623
Size: 283 1340
Bme1580I G_kGCm'C
Cuts at: 0 350 480 1020 1623
Size: 350 130 540 603
Fragments arranged by size:
603 540 350 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 261 699 1623
Size: 261 438 924
Fragments arranged by size:
924 438 261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1147 1623
Size: 1147 476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 488 1457 1623
Size: 88 400 969 166
Fragments arranged by size:
969 400 166 88
Bpu10I CC'TnA_GC
Cuts at: 0 524 1623
Size: 524 1099
BsaHI Gr'CG_yC
Cuts at: 0 221 856 1623
Size: 221 635 767
Fragments arranged by size:
767 635 221
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 1623
Size: 232 30 1361
Fragments arranged by size:
1361 232 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 195 375 390 1232 1235 1289 1623
Size: 195 180 15 842 3 54 334
Fragments arranged by size:
842 334 195 180 54 15 3
BseYI C'CCAG_C
Cuts at: 0 43 704 1623
Size: 43 661 919
Fragments arranged by size:
919 661 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1623
Size: 223 1400
BsiHKAI G_wGCw'C
Cuts at: 0 350 1623
Size: 350 1273
Bsp1286I G_dGCh'C
Cuts at: 0 74 350 480 538 1020 1623
Size: 74 276 130 58 482 603
Fragments arranged by size:
603 482 276 130 74 58
BspHI T'CATG_A
Cuts at: 0 915 1623
Size: 915 708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 1623
Size: 71 1552
BsrBI CCG'CTC
Cuts at: 0 20 1623
Size: 20 1603
BsrFI r'CCGG_y
Cuts at: 0 176 286 1623
Size: 176 110 1337
Fragments arranged by size:
1337 176 110
BssSI C'ACGA_G
Cuts at: 0 349 1623
Size: 349 1274
BstBI TT'CG_AA
Cuts at: 0 1152 1221 1623
Size: 1152 69 402
Fragments arranged by size:
1152 402 69
BstYI r'GATC_y
Cuts at: 0 14 1623
Size: 14 1609
Bsu36I CC'TnA_GG
Cuts at: 0 1380 1623
Size: 1380 243
BtsI GCAGTG_nn'
Cuts at: 0 22 972 1623
Size: 22 950 651
Fragments arranged by size:
950 651 22
DraI TTT'AAA
Cuts at: 0 1347 1623
Size: 1347 276
EaeI y'GGCC_r
Cuts at: 0 284 1623
Size: 284 1339
EarI CTCTTCn'nnn_
Cuts at: 0 938 1050 1116 1133 1623
Size: 938 112 66 17 490
Fragments arranged by size:
938 490 112 66 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 559 1623
Size: 559 1064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 144 208 488 1457 1623
Size: 88 56 64 280 969 166
Fragments arranged by size:
969 280 166 88 64 56
EcoO109I rG'GnC_Cy
Cuts at: 0 60 1623
Size: 60 1563
EcoRI G'AATT_C
Cuts at: 0 725 1623
Size: 725 898
HaeII r_GCGC'y
Cuts at: 0 224 859 1623
Size: 224 635 764
Fragments arranged by size:
764 635 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 567 599 961 993 1623
Size: 567 32 362 32 630
Fragments arranged by size:
630 567 362 32 32
HindIII A'AGCT_T
Cuts at: 0 143 595 831 1441 1465 1623
Size: 143 452 236 610 24 158
Fragments arranged by size:
610 452 236 158 143 24
KasI G'GCGC_C
Cuts at: 0 220 855 1623
Size: 220 635 768
Fragments arranged by size:
768 635 220
KpnI G_GTAC'C
Cuts at: 0 110 1623
Size: 110 1513
MluI A'CGCG_T
Cuts at: 0 1012 1623
Size: 1012 611
MslI CAynn'nnrTG
Cuts at: 0 1133 1623
Size: 1133 490
MspA1I CmG'CkG
Cuts at: 0 186 237 1623
Size: 186 51 1386
Fragments arranged by size:
1386 186 51
NaeI GCC'GGC
Cuts at: 0 288 1623
Size: 288 1335
NarI GG'CG_CC
Cuts at: 0 221 856 1623
Size: 221 635 767
Fragments arranged by size:
767 635 221
NgoMIV G'CCGG_C
Cuts at: 0 286 1623
Size: 286 1337
NsiI A_TGCA'T
Cuts at: 0 744 1623
Size: 744 879
NspI r_CATG'y
Cuts at: 0 746 1623
Size: 746 877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1439 1471 1623
Size: 1439 32 152
Fragments arranged by size:
1439 152 32
PpuMI rG'GwC_Cy
Cuts at: 0 60 1623
Size: 60 1563
PvuII CAG'CTG
Cuts at: 0 186 1623
Size: 186 1437
SexAI A'CCwGG_T
Cuts at: 0 1085 1623
Size: 1085 538
SfcI C'TryA_G
Cuts at: 0 181 1623
Size: 181 1442
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 283 1623
Size: 283 1340
SfoI GGC'GCC
Cuts at: 0 222 857 1623
Size: 222 635 766
Fragments arranged by size:
766 635 222
SmaI CCC'GGG
Cuts at: 0 75 1623
Size: 75 1548
SmlI C'TyrA_G
Cuts at: 0 598 1623
Size: 598 1025
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 696 1623
Size: 696 927
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 1623
Size: 232 32 1359
Fragments arranged by size:
1359 232 32
XbaI T'CTAG_A
Cuts at: 0 678 1623
Size: 678 945
XmaI C'CCGG_G
Cuts at: 0 73 1623
Size: 73 1550
XmnI GAAnn'nnTTC
Cuts at: 0 1454 1623
Size: 1454 169
Enzymes that do cut:
Acc65I AclI AcuI AflII AflIII AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BfrBI BglI Bme1580I
BmrI BplI BpmI Bpu10I BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI
BstBI BstYI Bsu36I BtsI DraI EaeI EarI EciI
Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII KasI KpnI
MluI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PpiI PpuMI PvuII SexAI SfcI SfiI SfoI SmaI
SmlI TaqII TstI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AgeI AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BglII BlpI BmgBI BmtI
BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstEII BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII
HpaI MfeI MmeI MscI NcoI NdeI NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI Tth111I XcmI XhoI ZraI