Restriction map for
pF1KB8703
(Linear) (Six-base) MAPSORT of: KIBB8703.seq Check: 6745 from: 1 to: 1722
KIBB8703.orf
With 182 enzymes: SgfI *
February 20, 2009 16:37 ..
AflIII A'CryG_T
Cuts at: 0 1223 1722
Size: 1223 499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 536 1722
Size: 536 1186
AlwNI CAG_nnn'CTG
Cuts at: 0 154 1255 1592 1722
Size: 154 1101 337 130
Fragments arranged by size:
1101 337 154 130
ApaI G_GGCC'C
Cuts at: 0 656 1722
Size: 656 1066
ApoI r'AATT_y
Cuts at: 0 967 1722
Size: 967 755
AvaI C'yCGr_G
Cuts at: 0 566 711 1722
Size: 566 145 1011
Fragments arranged by size:
1011 566 145
BanI G'GyrC_C
Cuts at: 0 455 534 783 1722
Size: 455 79 249 939
Fragments arranged by size:
939 455 249 79
BanII G_rGCy'C
Cuts at: 0 656 862 1722
Size: 656 206 860
Fragments arranged by size:
860 656 206
BbeI G_GCGC'C
Cuts at: 0 459 1722
Size: 459 1263
BbsI GAAGACnn'nnnn_
Cuts at: 0 85 1722
Size: 85 1637
BbvCI CC'TCA_GC
Cuts at: 0 216 614 1000 1722
Size: 216 398 386 722
Fragments arranged by size:
722 398 386 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 344 378 1722
Size: 344 34 1344
Fragments arranged by size:
1344 344 34
BglI GCCn_nnn'nGGC
Cuts at: 0 464 651 1722
Size: 464 187 1071
Fragments arranged by size:
1071 464 187
BglII A'GATC_T
Cuts at: 0 1685 1722
Size: 1685 37
Bme1580I G_kGCm'C
Cuts at: 0 647 656 1722
Size: 647 9 1066
Fragments arranged by size:
1066 647 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 650 1722
Size: 650 1072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1405 1722
Size: 1048 357 317
Fragments arranged by size:
1048 357 317
Bpu10I CC'TnA_GC
Cuts at: 0 38 216 614 1000 1475 1722
Size: 38 178 398 386 475 247
Fragments arranged by size:
475 398 386 247 178 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 109 1722
Size: 57 52 1613
Fragments arranged by size:
1613 57 52
BsaI GGTCTCn'nnnn_
Cuts at: 0 638 1226 1722
Size: 638 588 496
Fragments arranged by size:
638 588 496
BsaAI yAC'GTr
Cuts at: 0 1158 1722
Size: 1158 564
BsaHI Gr'CG_yC
Cuts at: 0 183 456 526 1722
Size: 183 273 70 1196
Fragments arranged by size:
1196 273 183 70
BsaWI w'CCGG_w
Cuts at: 0 398 1722
Size: 398 1324
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1223 1253 1722
Size: 1223 30 469
Fragments arranged by size:
1223 469 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 256 259 262 283 686 946 1722
Size: 244 12 3 3 21 403 260 776
Fragments arranged by size:
776 403 260 244 21 12 3 3
BseYI C'CCAG_C
Cuts at: 0 63 312 323 509 588 1655 1722
Size: 63 249 11 186 79 1067 67
Fragments arranged by size:
1067 249 186 79 67 63 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1722
Size: 840 882
BsiHKAI G_wGCw'C
Cuts at: 0 606 1722
Size: 606 1116
Bsp1286I G_dGCh'C
Cuts at: 0 606 647 656 862 1722
Size: 606 41 9 206 860
Fragments arranged by size:
860 606 206 41 9
BspEI T'CCGG_A
Cuts at: 0 398 1722
Size: 398 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 370 952 1722
Size: 370 582 770
Fragments arranged by size:
770 582 370
BsrDI GCAATG_nn'
Cuts at: 0 819 1122 1722
Size: 819 303 600
Fragments arranged by size:
819 600 303
BsrFI r'CCGG_y
Cuts at: 0 467 907 1722
Size: 467 440 815
Fragments arranged by size:
815 467 440
BstAPI GCAn_nnn'nTGC
Cuts at: 0 818 1665 1722
Size: 818 847 57
Fragments arranged by size:
847 818 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1341 1383 1722
Size: 1341 42 339
Fragments arranged by size:
1341 339 42
BstYI r'GATC_y
Cuts at: 0 150 1685 1722
Size: 150 1535 37
Fragments arranged by size:
1535 150 37
BtgI C'CryG_G
Cuts at: 0 659 1121 1166 1722
Size: 659 462 45 556
Fragments arranged by size:
659 556 462 45
BtsI GCAGTG_nn'
Cuts at: 0 1704 1722
Size: 1704 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 114 149 1722
Size: 114 35 1573
Fragments arranged by size:
1573 114 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 690 1722
Size: 690 1032
EaeI y'GGCC_r
Cuts at: 0 909 1568 1722
Size: 909 659 154
Fragments arranged by size:
909 659 154
EarI CTCTTCn'nnn_
Cuts at: 0 221 233 260 875 1722
Size: 221 12 27 615 847
Fragments arranged by size:
847 615 221 27 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1405 1722
Size: 1048 357 317
Fragments arranged by size:
1048 357 317
EcoNI CCTnn'n_nnAGG
Cuts at: 0 158 928 1722
Size: 158 770 794
Fragments arranged by size:
794 770 158
EcoO109I rG'GnC_Cy
Cuts at: 0 26 34 477 653 1071 1181 1722
Size: 26 8 443 176 418 110 541
Fragments arranged by size:
541 443 418 176 110 26 8
EcoRI G'AATT_C
Cuts at: 0 967 1722
Size: 967 755
HaeII r_GCGC'y
Cuts at: 0 375 459 1722
Size: 375 84 1263
Fragments arranged by size:
1263 375 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 694 726 1223 1255 1415 1447
Size: 142 32 520 32 497 32 160 32
Cuts at: 1447 1722
Size: 275
Fragments arranged by size:
520 497 275 160 142 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 445 1722
Size: 445 1277
KasI G'GCGC_C
Cuts at: 0 455 1722
Size: 455 1267
MscI TGG'CCA
Cuts at: 0 1570 1722
Size: 1570 152
MspA1I CmG'CkG
Cuts at: 0 312 327 1086 1104 1469 1722
Size: 312 15 759 18 365 253
Fragments arranged by size:
759 365 312 253 18 15
NaeI GCC'GGC
Cuts at: 0 469 909 1722
Size: 469 440 813
Fragments arranged by size:
813 469 440
NarI GG'CG_CC
Cuts at: 0 456 1722
Size: 456 1266
NcoI C'CATG_G
Cuts at: 0 659 1121 1166 1722
Size: 659 462 45 556
Fragments arranged by size:
659 556 462 45
NgoMIV G'CCGG_C
Cuts at: 0 467 907 1722
Size: 467 440 815
Fragments arranged by size:
815 467 440
NspI r_CATG'y
Cuts at: 0 940 1227 1668 1722
Size: 940 287 441 54
Fragments arranged by size:
940 441 287 54
PciI A'CATG_T
Cuts at: 0 1223 1722
Size: 1223 499
PflMI CCAn_nnn'nTGG
Cuts at: 0 154 1722
Size: 154 1568
PfoI T'CCnGG_A
Cuts at: 0 45 1063 1722
Size: 45 1018 659
Fragments arranged by size:
1018 659 45
PmlI CAC'GTG
Cuts at: 0 1158 1722
Size: 1158 564
PpuMI rG'GwC_Cy
Cuts at: 0 26 1181 1722
Size: 26 1155 541
Fragments arranged by size:
1155 541 26
PshAI GACnn'nnGTC
Cuts at: 0 126 1722
Size: 126 1596
PsiI TTA'TAA
Cuts at: 0 1148 1722
Size: 1148 574
PspOMI G'GGCC_C
Cuts at: 0 652 1722
Size: 652 1070
PvuII CAG'CTG
Cuts at: 0 312 1086 1722
Size: 312 774 636
Fragments arranged by size:
774 636 312
ScaI AGT'ACT
Cuts at: 0 1615 1722
Size: 1615 107
SexAI A'CCwGG_T
Cuts at: 0 1211 1235 1722
Size: 1211 24 487
Fragments arranged by size:
1211 487 24
SfcI C'TryA_G
Cuts at: 0 1495 1722
Size: 1495 227
SfoI GGC'GCC
Cuts at: 0 457 1722
Size: 457 1265
SmaI CCC'GGG
Cuts at: 0 568 1722
Size: 568 1154
SmlI C'TyrA_G
Cuts at: 0 72 124 1722
Size: 72 52 1598
Fragments arranged by size:
1598 72 52
StuI AGG'CCT
Cuts at: 0 894 1722
Size: 894 828
StyI C'CwwG_G
Cuts at: 0 659 1121 1166 1304 1722
Size: 659 462 45 138 418
Fragments arranged by size:
659 462 418 138 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1535 1669 1722
Size: 1535 134 53
Fragments arranged by size:
1535 134 53
TatI w'GTAC_w
Cuts at: 0 727 1613 1722
Size: 727 886 109
Fragments arranged by size:
886 727 109
Tth111I GACn'n_nGTC
Cuts at: 0 24 1237 1249 1722
Size: 24 1213 12 473
Fragments arranged by size:
1213 473 24 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1071 1173 1722
Size: 1071 102 549
Fragments arranged by size:
1071 549 102
XmaI C'CCGG_G
Cuts at: 0 566 1722
Size: 566 1156
XmnI GAAnn'nnTTC
Cuts at: 0 971 1722
Size: 971 751
Enzymes that do cut:
AflIII AlfI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI
BsrFI BstAPI BstXI BstYI BtgI BtsI CspCI DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HindIII KasI MscI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PvuII ScaI SexAI SfcI SfoI SmaI SmlI
StuI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BlpI BmgBI
BmrI BmtI BsaBI BsiEI BsiWI BsmI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DraIII EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HincII HpaI KpnI MfeI
MluI MmeI MslI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PpiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI ZraI