(Linear) (Six-base) MAPSORT of: KIBB8704.seq Check: 2910 from: 1 to: 714 KIBB8704.orf With 182 enzymes: SgfI * July 15, 2008 09:38 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 488 635 714 Size: 212 276 147 79 Fragments arranged by size: 276 212 147 79 AlwNI CAG_nnn'CTG Cuts at: 0 280 714 Size: 280 434 ApoI r'AATT_y Cuts at: 0 110 714 Size: 110 604 BanI G'GyrC_C Cuts at: 0 250 714 Size: 250 464 BanII G_rGCy'C Cuts at: 0 302 714 Size: 302 412 BbvCI CC'TCA_GC Cuts at: 0 185 714 Size: 185 529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 493 527 714 Size: 493 34 187 Fragments arranged by size: 493 187 34 BglI GCCn_nnn'nGGC Cuts at: 0 376 714 Size: 376 338 BlpI GC'TnA_GC Cuts at: 0 408 714 Size: 408 306 Bme1580I G_kGCm'C Cuts at: 0 663 714 Size: 663 51 BmrI ACTGGGnnnn_n' Cuts at: 0 605 714 Size: 605 109 BmtI G_CTAG'C Cuts at: 0 122 714 Size: 122 592 Bpu10I CC'TnA_GC Cuts at: 0 185 714 Size: 185 529 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 343 409 502 714 Size: 343 66 93 212 Fragments arranged by size: 343 212 93 66 BseYI C'CCAG_C Cuts at: 0 273 477 578 714 Size: 273 204 101 136 Fragments arranged by size: 273 204 136 101 Bsp1286I G_dGCh'C Cuts at: 0 302 663 714 Size: 302 361 51 Fragments arranged by size: 361 302 51 BsrFI r'CCGG_y Cuts at: 0 403 714 Size: 403 311 BstAPI GCAn_nnn'nTGC Cuts at: 0 556 714 Size: 556 158 Bsu36I CC'TnA_GG Cuts at: 0 650 692 714 Size: 650 42 22 Fragments arranged by size: 650 42 22 BtgI C'CryG_G Cuts at: 0 126 714 Size: 126 588 BtsI GCAGTG_nn' Cuts at: 0 521 714 Size: 521 193 EarI CTCTTCn'nnn_ Cuts at: 0 611 677 714 Size: 611 66 37 Fragments arranged by size: 611 66 37 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 488 635 714 Size: 212 276 147 79 Fragments arranged by size: 276 212 147 79 HindIII A'AGCT_T Cuts at: 0 434 714 Size: 434 280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 610 714 Size: 610 104 MspA1I CmG'CkG Cuts at: 0 412 714 Size: 412 302 NaeI GCC'GGC Cuts at: 0 405 714 Size: 405 309 NgoMIV G'CCGG_C Cuts at: 0 403 714 Size: 403 311 NheI G'CTAG_C Cuts at: 0 118 714 Size: 118 596 NspI r_CATG'y Cuts at: 0 712 714 Size: 712 2 PflMI CCAn_nnn'nTGG Cuts at: 0 280 714 Size: 280 434 PvuII CAG'CTG Cuts at: 0 412 714 Size: 412 302 SfcI C'TryA_G Cuts at: 0 632 714 Size: 632 82 StyI C'CwwG_G Cuts at: 0 461 482 566 714 Size: 461 21 84 148 Fragments arranged by size: 461 148 84 21 Enzymes that do cut: AcuI AlwNI ApoI BanI BanII BbvCI BcgI BglI BlpI Bme1580I BmrI BmtI Bpu10I BseRI BseYI Bsp1286I BsrFI BstAPI Bsu36I BtgI BtsI EarI Eco57MI HindIII MmeI MspA1I NaeI NgoMIV NheI NspI PflMI PvuII SfcI StyI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglII BmgBI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MscI MslI NarI NcoI NdeI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI