(Linear) (Six-base) MAPSORT of: KIBB8704.seq  Check: 2910  from: 1  to: 714



KIBB8704.orf

 With 182 enzymes: SgfI * 

                        July 15, 2008 09:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     488     635     714 
   Size:        212     276     147      79

  Fragments arranged by size: 

                 276     212     147      79
 
AlwNI CAG_nnn'CTG

Cuts at:      0     280     714 
   Size:        280     434
 
ApoI r'AATT_y

Cuts at:      0     110     714 
   Size:        110     604
 
BanI G'GyrC_C

Cuts at:      0     250     714 
   Size:        250     464
 
BanII G_rGCy'C

Cuts at:      0     302     714 
   Size:        302     412
 
BbvCI CC'TCA_GC

Cuts at:      0     185     714 
   Size:        185     529
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     493     527     714 
   Size:        493      34     187

  Fragments arranged by size: 

                 493     187      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     376     714 
   Size:        376     338
 
BlpI GC'TnA_GC

Cuts at:      0     408     714 
   Size:        408     306
 
Bme1580I G_kGCm'C

Cuts at:      0     663     714 
   Size:        663      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     605     714 
   Size:        605     109
 
BmtI G_CTAG'C

Cuts at:      0     122     714 
   Size:        122     592
 
Bpu10I CC'TnA_GC

Cuts at:      0     185     714 
   Size:        185     529
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     409     502     714 
   Size:        343      66      93     212

  Fragments arranged by size: 

                 343     212      93      66
 
BseYI C'CCAG_C

Cuts at:      0     273     477     578     714 
   Size:        273     204     101     136

  Fragments arranged by size: 

                 273     204     136     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     302     663     714 
   Size:        302     361      51

  Fragments arranged by size: 

                 361     302      51
 
BsrFI r'CCGG_y

Cuts at:      0     403     714 
   Size:        403     311
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     556     714 
   Size:        556     158
 
Bsu36I CC'TnA_GG

Cuts at:      0     650     692     714 
   Size:        650      42      22

  Fragments arranged by size: 

                 650      42      22
 
BtgI C'CryG_G

Cuts at:      0     126     714 
   Size:        126     588
 
BtsI GCAGTG_nn'

Cuts at:      0     521     714 
   Size:        521     193
 
EarI CTCTTCn'nnn_

Cuts at:      0     611     677     714 
   Size:        611      66      37

  Fragments arranged by size: 

                 611      66      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     488     635     714 
   Size:        212     276     147      79

  Fragments arranged by size: 

                 276     212     147      79
 
HindIII A'AGCT_T

Cuts at:      0     434     714 
   Size:        434     280
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     610     714 
   Size:        610     104
 
MspA1I CmG'CkG

Cuts at:      0     412     714 
   Size:        412     302
 
NaeI GCC'GGC

Cuts at:      0     405     714 
   Size:        405     309
 
NgoMIV G'CCGG_C

Cuts at:      0     403     714 
   Size:        403     311
 
NheI G'CTAG_C

Cuts at:      0     118     714 
   Size:        118     596
 
NspI r_CATG'y

Cuts at:      0     712     714 
   Size:        712       2
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     280     714 
   Size:        280     434
 
PvuII CAG'CTG

Cuts at:      0     412     714 
   Size:        412     302
 
SfcI C'TryA_G

Cuts at:      0     632     714 
   Size:        632      82
 
StyI C'CwwG_G

Cuts at:      0     461     482     566     714 
   Size:        461      21      84     148

  Fragments arranged by size: 

                 461     148      84      21
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI    BanII    BbvCI     BcgI     BglI
     BlpI Bme1580I     BmrI     BmtI   Bpu10I    BseRI    BseYI Bsp1286I
    BsrFI   BstAPI   Bsu36I     BtgI     BtsI     EarI  Eco57MI  HindIII
     MmeI   MspA1I     NaeI   NgoMIV     NheI     NspI    PflMI    PvuII
     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BciVI     BclI    BfrBI    BglII    BmgBI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NarI     NcoI     NdeI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI