Restriction map for
pF1KB8705
(Linear) (Six-base) MAPSORT of: KIBB8705.seq Check: 1859 from: 1 to: 1128
KIBB8705.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AflIII A'CryG_T
Cuts at: 0 209 1128
Size: 209 919
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 972 1004 1128
Size: 972 32 124
Fragments arranged by size:
972 124 32
AlwNI CAG_nnn'CTG
Cuts at: 0 253 1128
Size: 253 875
ApoI r'AATT_y
Cuts at: 0 777 1128
Size: 777 351
AseI AT'TA_AT
Cuts at: 0 877 1018 1128
Size: 877 141 110
Fragments arranged by size:
877 141 110
AvaI C'yCGr_G
Cuts at: 0 66 1128
Size: 66 1062
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 925 958 1128
Size: 925 33 170
Fragments arranged by size:
925 170 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 242 1128
Size: 208 34 886
Fragments arranged by size:
886 208 34
BclI T'GATC_A
Cuts at: 0 618 1128
Size: 618 510
BglI GCCn_nnn'nGGC
Cuts at: 0 235 1128
Size: 235 893
BglII A'GATC_T
Cuts at: 0 62 1128
Size: 62 1066
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 908 1128
Size: 908 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 383 1106 1128
Size: 192 191 723 22
Fragments arranged by size:
723 192 191 22
Bpu10I CC'TnA_GC
Cuts at: 0 183 1128
Size: 183 945
BsaAI yAC'GTr
Cuts at: 0 1104 1128
Size: 1104 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 881 1128
Size: 881 247
BseYI C'CCAG_C
Cuts at: 0 685 1128
Size: 685 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 1128
Size: 590 538
BsiHKAI G_wGCw'C
Cuts at: 0 546 1128
Size: 546 582
Bsp1286I G_dGCh'C
Cuts at: 0 546 1128
Size: 546 582
BspHI T'CATG_A
Cuts at: 0 651 767 1128
Size: 651 116 361
Fragments arranged by size:
651 361 116
BsrBI CCG'CTC
Cuts at: 0 307 1128
Size: 307 821
BsrDI GCAATG_nn'
Cuts at: 0 573 1128
Size: 573 555
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1033 1128
Size: 1033 95
BstYI r'GATC_y
Cuts at: 0 62 798 1128
Size: 62 736 330
Fragments arranged by size:
736 330 62
BtgI C'CryG_G
Cuts at: 0 513 1128
Size: 513 615
BtsI GCAGTG_nn'
Cuts at: 0 224 350 1128
Size: 224 126 778
Fragments arranged by size:
778 224 126
EaeI y'GGCC_r
Cuts at: 0 510 561 1128
Size: 510 51 567
Fragments arranged by size:
567 510 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 383 1106 1128
Size: 192 191 723 22
Fragments arranged by size:
723 192 191 22
EcoO109I rG'GnC_Cy
Cuts at: 0 682 1007 1128
Size: 682 325 121
Fragments arranged by size:
682 325 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 646 678 783 815 1128
Size: 271 32 343 32 105 32 313
Fragments arranged by size:
343 313 271 105 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 221 849 1128
Size: 116 105 628 279
Fragments arranged by size:
628 279 116 105
MscI TGG'CCA
Cuts at: 0 512 563 1128
Size: 512 51 565
Fragments arranged by size:
565 512 51
MslI CAynn'nnrTG
Cuts at: 0 500 563 584 614 620 626 632 650
Size: 500 63 21 30 6 6 6 18
Cuts at: 650 769 1128
Size: 119 359
Fragments arranged by size:
500 359 119 63 30 21 18 6
6 6
NspI r_CATG'y
Cuts at: 0 213 1128
Size: 213 915
PciI A'CATG_T
Cuts at: 0 209 1128
Size: 209 919
PfoI T'CCnGG_A
Cuts at: 0 103 1128
Size: 103 1025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 296 328 1128
Size: 296 32 800
Fragments arranged by size:
800 296 32
PpuMI rG'GwC_Cy
Cuts at: 0 682 1007 1128
Size: 682 325 121
Fragments arranged by size:
682 325 121
SnaBI TAC'GTA
Cuts at: 0 1104 1128
Size: 1104 24
SspI AAT'ATT
Cuts at: 0 467 875 1071 1128
Size: 467 408 196 57
Fragments arranged by size:
467 408 196 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 54 1128
Size: 54 1074
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 1128
Size: 511 32 585
Fragments arranged by size:
585 511 32
Tth111I GACn'n_nGTC
Cuts at: 0 521 1128
Size: 521 607
XbaI T'CTAG_A
Cuts at: 0 546 1128
Size: 546 582
Enzymes that do cut:
AflIII AloI AlwNI ApoI AseI AvaI BaeI BcgI
BclI BglI BglII BplI BpmI Bpu10I BsaAI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BstAPI
BstYI BtgI BtsI EaeI Eco57MI EcoO109I Hin4I MmeI
MscI MslI NspI PciI PfoI PpiI PpuMI SnaBI
SspI TaqII TstI Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BfrBI BlpI Bme1580I BmgBI BmrI BmtI BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI
SwaI TatI XcmI XhoI XmaI XmnI ZraI