(Linear) (Six-base) MAPSORT of: KIBB8706.seq  Check: 2419  from: 1  to: 1470



KIBB8706.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     839    1470 
   Size:        488     351     631

  Fragments arranged by size: 

                 631     488     351
 
AflII C'TTAA_G

Cuts at:      0     823    1470 
   Size:        823     647
 
AhdI GACnn_n'nnGTC

Cuts at:      0      18    1470 
   Size:         18    1452
 
ApoI r'AATT_y

Cuts at:      0     707     870    1278    1345    1470 
   Size:        707     163     408      67     125

  Fragments arranged by size: 

                 707     408     163     125      67
 
AvrII C'CTAG_G

Cuts at:      0    1383    1470 
   Size:       1383      87
 
BanII G_rGCy'C

Cuts at:      0     435    1195    1470 
   Size:        435     760     275

  Fragments arranged by size: 

                 760     435     275
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     698     755    1470 
   Size:        698      57     715

  Fragments arranged by size: 

                 715     698      57
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     890    1470 
   Size:        890     580
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1231    1470 
   Size:       1231     239
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1214    1470 
   Size:       1214     256
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1470 
   Size:        724     746
 
BseYI C'CCAG_C

Cuts at:      0     214    1272    1470 
   Size:        214    1058     198

  Fragments arranged by size: 

                1058     214     198
 
BsiHKAI G_wGCw'C

Cuts at:      0     193    1195    1470 
   Size:        193    1002     275

  Fragments arranged by size: 

                1002     275     193
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     738    1470 
   Size:        738     732
 
Bsp1286I G_dGCh'C

Cuts at:      0     193     435    1195    1470 
   Size:        193     242     760     275

  Fragments arranged by size: 

                 760     275     242     193
 
BsrDI GCAATG_nn'

Cuts at:      0     258     980    1470 
   Size:        258     722     490

  Fragments arranged by size: 

                 722     490     258
 
BsrGI T'GTAC_A

Cuts at:      0     622    1470 
   Size:        622     848
 
BtsI GCAGTG_nn'

Cuts at:      0     212    1470 
   Size:        212    1258
 
EcoICRI GAG'CTC

Cuts at:      0    1193    1470 
   Size:       1193     277
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     724     839    1470 
   Size:        488     236     115     631

  Fragments arranged by size: 

                 631     488     236     115
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     418    1001    1470 
   Size:        418     583     469

  Fragments arranged by size: 

                 583     469     418
 
EcoO109I rG'GnC_Cy

Cuts at:      0     994    1380    1470 
   Size:        994     386      90

  Fragments arranged by size: 

                 994     386      90
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36    1053    1085    1470 
   Size:          4      32    1017      32     385

  Fragments arranged by size: 

                1017     385      32      32       4
 
HincII GTy'rAC

Cuts at:      0     168    1470 
   Size:        168    1302
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     773    1470 
   Size:        371     402     697

  Fragments arranged by size: 

                 697     402     371
 
MslI CAynn'nnrTG

Cuts at:      0     655    1147    1470 
   Size:        655     492     323

  Fragments arranged by size: 

                 655     492     323
 
MspA1I CmG'CkG

Cuts at:      0     214     886    1470 
   Size:        214     672     584

  Fragments arranged by size: 

                 672     584     214
 
PshAI GACnn'nnGTC

Cuts at:      0     451    1470 
   Size:        451    1019
 
PstI C_TGCA'G

Cuts at:      0    1359    1470 
   Size:       1359     111
 
PvuII CAG'CTG

Cuts at:      0     214    1470 
   Size:        214    1256
 
SacI G_AGCT'C

Cuts at:      0    1195    1470 
   Size:       1195     275
 
SfcI C'TryA_G

Cuts at:      0    1098    1355    1470 
   Size:       1098     257     115

  Fragments arranged by size: 

                1098     257     115
 
SmlI C'TyrA_G

Cuts at:      0     823    1470 
   Size:        823     647
 
SspI AAT'ATT

Cuts at:      0     945    1470 
   Size:        945     525
 
StyI C'CwwG_G

Cuts at:      0    1383    1470 
   Size:       1383      87
 
TatI w'GTAC_w

Cuts at:      0     622    1470 
   Size:        622     848
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1157    1189    1470 
   Size:       1157      32     281

  Fragments arranged by size: 

                1157     281      32
 

 Enzymes that do cut:

     AcuI    AflII     AhdI     ApoI    AvrII    BanII     BbsI    BciVI
     BmrI     BplI     BpmI    BseYI  BsiHKAI    BsmBI Bsp1286I    BsrDI
    BsrGI     BtsI  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII
     MmeI     MslI   MspA1I    PshAI     PstI    PvuII     SacI     SfcI
     SmlI     SspI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI