Restriction map for
pF1KB8707
(Linear) (Six-base) MAPSORT of: KIBB8707.seq Check: 1921 from: 1 to: 1617
KIBB8707.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 0 1521 1617
Size: 1521 96
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1565 1617
Size: 1565 52
AflII C'TTAA_G
Cuts at: 0 549 1617
Size: 549 1068
AlwNI CAG_nnn'CTG
Cuts at: 0 1471 1617
Size: 1471 146
ApoI r'AATT_y
Cuts at: 0 268 473 664 838 1203 1617
Size: 268 205 191 174 365 414
Fragments arranged by size:
414 365 268 205 191 174
AvrII C'CTAG_G
Cuts at: 0 865 1617
Size: 865 752
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 992 1617
Size: 80 912 625
Fragments arranged by size:
912 625 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 570 604 1617
Size: 570 34 1013
Fragments arranged by size:
1013 570 34
BfrBI ATG'CAT
Cuts at: 0 561 1617
Size: 561 1056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1617
Size: 764 853
BsaI GGTCTCn'nnnn_
Cuts at: 0 1520 1617
Size: 1520 97
BsaAI yAC'GTr
Cuts at: 0 1375 1617
Size: 1375 242
BsaHI Gr'CG_yC
Cuts at: 0 1320 1617
Size: 1320 297
BsaWI w'CCGG_w
Cuts at: 0 1189 1617
Size: 1189 428
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 176 206 1617
Size: 176 30 1411
Fragments arranged by size:
1411 176 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1617
Size: 1060 557
BsiHKAI G_wGCw'C
Cuts at: 0 1406 1617
Size: 1406 211
BsiWI C'GTAC_G
Cuts at: 0 577 1617
Size: 577 1040
BsmI GAATG_Cn'
Cuts at: 0 634 1617
Size: 634 983
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1311 1617
Size: 1311 306
Bsp1286I G_dGCh'C
Cuts at: 0 1406 1617
Size: 1406 211
BspHI T'CATG_A
Cuts at: 0 69 657 1617
Size: 69 588 960
Fragments arranged by size:
960 588 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1032 1617
Size: 1032 585
BstXI CCAn_nnnn'nTGG
Cuts at: 0 233 1617
Size: 233 1384
BstYI r'GATC_y
Cuts at: 0 1410 1617
Size: 1410 207
Bsu36I CC'TnA_GG
Cuts at: 0 401 1617
Size: 401 1216
DraI TTT'AAA
Cuts at: 0 381 1617
Size: 381 1236
DraIII CAC_nnn'GTG
Cuts at: 0 1378 1617
Size: 1378 239
EarI CTCTTCn'nnn_
Cuts at: 0 529 602 1149 1326 1617
Size: 529 73 547 177 291
Fragments arranged by size:
547 529 291 177 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1617
Size: 19 1598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1565 1617
Size: 764 801 52
Fragments arranged by size:
801 764 52
EcoO109I rG'GnC_Cy
Cuts at: 0 1174 1617
Size: 1174 443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 97 1617
Size: 97 1520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 530 562 1617
Size: 530 32 1055
Fragments arranged by size:
1055 530 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 858 1617
Size: 858 759
MslI CAynn'nnrTG
Cuts at: 0 231 1304 1617
Size: 231 1073 313
Fragments arranged by size:
1073 313 231
MspA1I CmG'CkG
Cuts at: 0 1122 1617
Size: 1122 495
NsiI A_TGCA'T
Cuts at: 0 563 1617
Size: 563 1054
NspI r_CATG'y
Cuts at: 0 470 561 1617
Size: 470 91 1056
Fragments arranged by size:
1056 470 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 1378 1617
Size: 1378 239
PfoI T'CCnGG_A
Cuts at: 0 741 1617
Size: 741 876
PmlI CAC'GTG
Cuts at: 0 1375 1617
Size: 1375 242
PpuMI rG'GwC_Cy
Cuts at: 0 1174 1617
Size: 1174 443
PstI C_TGCA'G
Cuts at: 0 1467 1617
Size: 1467 150
PvuII CAG'CTG
Cuts at: 0 1122 1617
Size: 1122 495
ScaI AGT'ACT
Cuts at: 0 1497 1617
Size: 1497 120
SfcI C'TryA_G
Cuts at: 0 1105 1463 1617
Size: 1105 358 154
Fragments arranged by size:
1105 358 154
SmlI C'TyrA_G
Cuts at: 0 549 1617
Size: 549 1068
SphI G_CATG'C
Cuts at: 0 470 1617
Size: 470 1147
StyI C'CwwG_G
Cuts at: 0 865 1617
Size: 865 752
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 221 1617
Size: 221 1396
TatI w'GTAC_w
Cuts at: 0 1495 1617
Size: 1495 122
XbaI T'CTAG_A
Cuts at: 0 1050 1617
Size: 1050 567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 226 233 1617
Size: 226 7 1384
Fragments arranged by size:
1384 226 7
XmnI GAAnn'nnTTC
Cuts at: 0 332 1344 1617
Size: 332 1012 273
Fragments arranged by size:
1012 332 273
Enzymes that do cut:
AccI AcuI AflII AlwNI ApoI AvrII BbsI BcgI
BfrBI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BsgI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BstXI
BstYI Bsu36I DraI DraIII EarI EciI Eco57MI EcoO109I
FalI Hin4I MmeI MslI MspA1I NsiI NspI PflMI
PfoI PmlI PpuMI PstI PvuII ScaI SfcI SmlI
SphI StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaBI BseRI BseYI BsiEI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgI BtgZI BtsI ClaI CspCI DrdI
EaeI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XhoI XmaI ZraI