Restriction map for
pF1KB8713
(Linear) (Six-base) MAPSORT of: KIBB8713.seq Check: 9071 from: 1 to: 927
KIBB8713.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 543 585 690 795 837 927
Size: 543 42 105 105 42 90
Fragments arranged by size:
543 105 105 90 42 42
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 927
Size: 333 594
ApaLI G'TGCA_C
Cuts at: 0 489 927
Size: 489 438
ApoI r'AATT_y
Cuts at: 0 370 458 927
Size: 370 88 469
Fragments arranged by size:
469 370 88
AvaI C'yCGr_G
Cuts at: 0 410 927
Size: 410 517
BanII G_rGCy'C
Cuts at: 0 42 92 531 552 573 594 657 678
Size: 42 50 439 21 21 21 63 21
Cuts at: 678 699 720 762 783 927
Size: 21 21 42 21 144
Fragments arranged by size:
439 144 63 50 42 42 21 21
21 21 21 21 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 927
Size: 339 588
BciVI GTATCCnnnnn_n'
Cuts at: 0 556 619 661 808 927
Size: 556 63 42 147 119
Fragments arranged by size:
556 147 119 63 42
Bme1580I G_kGCm'C
Cuts at: 0 493 927
Size: 493 434
BmrI ACTGGGnnnn_n'
Cuts at: 0 372 482 927
Size: 372 110 445
Fragments arranged by size:
445 372 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 80 927
Size: 80 847
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 897 927
Size: 897 30
BseYI C'CCAG_C
Cuts at: 0 317 927
Size: 317 610
BsiHKAI G_wGCw'C
Cuts at: 0 92 493 927
Size: 92 401 434
Fragments arranged by size:
434 401 92
Bsp1286I G_dGCh'C
Cuts at: 0 42 92 493 531 552 573 594 657
Size: 42 50 401 38 21 21 21 63
Cuts at: 657 678 699 720 762 783 927
Size: 21 21 21 42 21 144
Fragments arranged by size:
401 144 63 50 42 42 38 21
21 21 21 21 21 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 543 585 690 795 837 927
Size: 543 42 105 105 42 90
Fragments arranged by size:
543 105 105 90 42 42
Bsu36I CC'TnA_GG
Cuts at: 0 851 927
Size: 851 76
BtgI C'CryG_G
Cuts at: 0 33 927
Size: 33 894
DraI TTT'AAA
Cuts at: 0 339 927
Size: 339 588
EarI CTCTTCn'nnn_
Cuts at: 0 899 915 927
Size: 899 16 12
Fragments arranged by size:
899 16 12
EcoICRI GAG'CTC
Cuts at: 0 90 927
Size: 90 837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 927
Size: 333 594
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 927
Size: 29 32 866
Fragments arranged by size:
866 32 29
MspA1I CmG'CkG
Cuts at: 0 35 927
Size: 35 892
NdeI CA'TA_TG
Cuts at: 0 309 927
Size: 309 618
PflMI CCAn_nnn'nTGG
Cuts at: 0 313 384 664 727 927
Size: 313 71 280 63 200
Fragments arranged by size:
313 280 200 71 63
PfoI T'CCnGG_A
Cuts at: 0 558 927
Size: 558 369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 729 761 927
Size: 729 32 166
Fragments arranged by size:
729 166 32
PstI C_TGCA'G
Cuts at: 0 540 582 687 792 927
Size: 540 42 105 105 135
Fragments arranged by size:
540 135 105 105 42
SacI G_AGCT'C
Cuts at: 0 92 927
Size: 92 835
SacII CC_GC'GG
Cuts at: 0 36 927
Size: 36 891
SbfI CC_TGCA'GG
Cuts at: 0 540 582 687 792 927
Size: 540 42 105 105 135
Fragments arranged by size:
540 135 105 105 42
SfcI C'TryA_G
Cuts at: 0 536 578 683 788 860 927
Size: 536 42 105 105 72 67
Fragments arranged by size:
536 105 105 72 67 42
StuI AGG'CCT
Cuts at: 0 849 927
Size: 849 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 314 927
Size: 314 613
Enzymes that do cut:
AarI AcuI ApaLI ApoI AvaI BanII BbsI BciVI
Bme1580I BmrI BsaI BseRI BseYI BsiHKAI Bsp1286I BspMI
Bsu36I BtgI DraI EarI EcoICRI Eco57MI Hin4I MspA1I
NdeI PflMI PfoI PpiI PstI SacI SacII SbfI
SfcI StuI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI