Restriction map for
pF1KB8714
(Linear) (Six-base) MAPSORT of: KIBB8714.seq Check: 7588 from: 1 to: 1329
KIBB8714.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 466 1329
Size: 148 318 863
Fragments arranged by size:
863 318 148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 1329
Size: 382 947
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 94 126 1329
Size: 94 32 1203
Fragments arranged by size:
1203 94 32
AlwNI CAG_nnn'CTG
Cuts at: 0 104 787 1329
Size: 104 683 542
Fragments arranged by size:
683 542 104
ApaI G_GGCC'C
Cuts at: 0 617 652 688 779 908 1231 1329
Size: 617 35 36 91 129 323 98
Fragments arranged by size:
617 323 129 98 91 36 35
AvaI C'yCGr_G
Cuts at: 0 34 48 610 1329
Size: 34 14 562 719
Fragments arranged by size:
719 562 34 14
BanI G'GyrC_C
Cuts at: 0 154 721 1243 1329
Size: 154 567 522 86
Fragments arranged by size:
567 522 154 86
BanII G_rGCy'C
Cuts at: 0 41 347 617 652 688 700 779 908
Size: 41 306 270 35 36 12 79 129
Cuts at: 908 1199 1231 1329
Size: 291 32 98
Fragments arranged by size:
306 291 270 129 98 79 41 36
35 32 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 301 335 1329
Size: 301 34 994
Fragments arranged by size:
994 301 34
BglI GCCn_nnn'nGGC
Cuts at: 0 756 1055 1235 1329
Size: 756 299 180 94
Fragments arranged by size:
756 299 180 94
Bme1580I G_kGCm'C
Cuts at: 0 296 617 652 688 724 779 908 1231
Size: 296 321 35 36 36 55 129 323
Cuts at: 1231 1329
Size: 98
Fragments arranged by size:
323 321 296 129 98 55 36 36
35
BmrI ACTGGGnnnn_n'
Cuts at: 0 893 1125 1329
Size: 893 232 204
Fragments arranged by size:
893 232 204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 758 1329
Size: 758 571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 565 731 749 875 884 1329
Size: 475 90 166 18 126 9 445
Fragments arranged by size:
475 445 166 126 90 18 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 225 818 847 1088 1329
Size: 225 593 29 241 241
Fragments arranged by size:
593 241 241 225 29
BsaWI w'CCGG_w
Cuts at: 0 91 1292 1329
Size: 91 1201 37
Fragments arranged by size:
1201 91 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 334 364 873 903 942 972
Size: 94 30 210 30 509 30 39 30
Cuts at: 972 1329
Size: 357
Fragments arranged by size:
509 357 210 94 39 30 30 30
30
BseYI C'CCAG_C
Cuts at: 0 780 815 1063 1212 1249 1329
Size: 780 35 248 149 37 80
Fragments arranged by size:
780 248 149 80 37 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1329
Size: 166 1163
BsiHKAI G_wGCw'C
Cuts at: 0 112 1329
Size: 112 1217
Bsp1286I G_dGCh'C
Cuts at: 0 41 112 296 347 617 652 688 700
Size: 41 71 184 51 270 35 36 12
Cuts at: 700 724 779 908 1199 1231 1329
Size: 24 55 129 291 32 98
Fragments arranged by size:
291 270 184 129 98 71 55 51
41 36 35 32 24 12
BspEI T'CCGG_A
Cuts at: 0 91 1329
Size: 91 1238
BsrBI CCG'CTC
Cuts at: 0 441 534 1329
Size: 441 93 795
Fragments arranged by size:
795 441 93
BsrDI GCAATG_nn'
Cuts at: 0 829 1329
Size: 829 500
BsrFI r'CCGG_y
Cuts at: 0 619 637 1234 1329
Size: 619 18 597 95
Fragments arranged by size:
619 597 95 18
BsrGI T'GTAC_A
Cuts at: 0 251 1329
Size: 251 1078
BstEII G'GTnAC_C
Cuts at: 0 120 1329
Size: 120 1209
BstYI r'GATC_y
Cuts at: 0 809 1329
Size: 809 520
Bsu36I CC'TnA_GG
Cuts at: 0 518 1088 1329
Size: 518 570 241
Fragments arranged by size:
570 518 241
BtgI C'CryG_G
Cuts at: 0 10 908 1329
Size: 10 898 421
Fragments arranged by size:
898 421 10
EaeI y'GGCC_r
Cuts at: 0 702 1002 1329
Size: 702 300 327
Fragments arranged by size:
702 327 300
EarI CTCTTCn'nnn_
Cuts at: 0 1102 1329
Size: 1102 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 387 1289 1329
Size: 387 902 40
Fragments arranged by size:
902 387 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 466 475 565 731 749 875 884
Size: 148 318 9 90 166 18 126 9
Cuts at: 884 1329
Size: 445
Fragments arranged by size:
445 318 166 148 126 90 18 9
9
EcoO109I rG'GnC_Cy
Cuts at: 0 522 541 648 684 685 739 757 776
Size: 522 19 107 36 1 54 18 19
Cuts at: 776 877 895 904 913 967 976 1228 1329
Size: 101 18 9 9 54 9 252 101
Fragments arranged by size:
522 252 107 101 101 54 54 36
19 19 18 18 9 9 9 1
HaeII r_GCGC'y
Cuts at: 0 25 62 382 1329
Size: 25 37 320 947
Fragments arranged by size:
947 320 37 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 942 974 1201 1233 1329
Size: 942 32 227 32 96
Fragments arranged by size:
942 227 96 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 863 1329
Size: 526 337 466
Fragments arranged by size:
526 466 337
MscI TGG'CCA
Cuts at: 0 1004 1329
Size: 1004 325
MspA1I CmG'CkG
Cuts at: 0 12 1071 1329
Size: 12 1059 258
Fragments arranged by size:
1059 258 12
NaeI GCC'GGC
Cuts at: 0 639 1236 1329
Size: 639 597 93
Fragments arranged by size:
639 597 93
NcoI C'CATG_G
Cuts at: 0 908 1329
Size: 908 421
NgoMIV G'CCGG_C
Cuts at: 0 637 1234 1329
Size: 637 597 95
Fragments arranged by size:
637 597 95
NspI r_CATG'y
Cuts at: 0 1282 1329
Size: 1282 47
PasI CC'CwG_GG
Cuts at: 0 289 555 691 772 873 919 946 1329
Size: 289 266 136 81 101 46 27 383
Fragments arranged by size:
383 289 266 136 101 81 46 27
PfoI T'CCnGG_A
Cuts at: 0 581 1329
Size: 581 748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 1329
Size: 94 32 1203
Fragments arranged by size:
1203 94 32
PpuMI rG'GwC_Cy
Cuts at: 0 522 541 739 877 913 967 1329
Size: 522 19 198 138 36 54 362
Fragments arranged by size:
522 362 198 138 54 36 19
PspOMI G'GGCC_C
Cuts at: 0 613 648 684 775 904 1227 1329
Size: 613 35 36 91 129 323 102
Fragments arranged by size:
613 323 129 102 91 36 35
PstI C_TGCA'G
Cuts at: 0 179 418 1329
Size: 179 239 911
Fragments arranged by size:
911 239 179
SacII CC_GC'GG
Cuts at: 0 13 1329
Size: 13 1316
SanDI GG'GwC_CC
Cuts at: 0 541 877 913 1329
Size: 541 336 36 416
Fragments arranged by size:
541 416 336 36
SapI GCTCTTCn'nnn_
Cuts at: 0 1102 1329
Size: 1102 227
SexAI A'CCwGG_T
Cuts at: 0 672 1329
Size: 672 657
SfcI C'TryA_G
Cuts at: 0 175 216 414 1329
Size: 175 41 198 915
Fragments arranged by size:
915 198 175 41
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 756 1329
Size: 756 573
SmaI CCC'GGG
Cuts at: 0 50 612 1329
Size: 50 562 717
Fragments arranged by size:
717 562 50
StuI AGG'CCT
Cuts at: 0 578 1329
Size: 578 751
StyI C'CwwG_G
Cuts at: 0 338 626 908 1053 1329
Size: 338 288 282 145 276
Fragments arranged by size:
338 288 282 276 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1022 1329
Size: 1022 307
TatI w'GTAC_w
Cuts at: 0 251 1329
Size: 251 1078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 334 366 873 905 1329
Size: 334 32 507 32 424
Fragments arranged by size:
507 424 334 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 589 1329
Size: 589 740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 148 372 880 1060 1329
Size: 148 224 508 180 269
Fragments arranged by size:
508 269 224 180 148
XmaI C'CCGG_G
Cuts at: 0 48 610 1329
Size: 48 562 719
Fragments arranged by size:
719 562 48
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApaI AvaI BanI BanII
BcgI BglI Bme1580I BmrI BplI BpmI BsaI BsaWI
BsaXI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrBI BsrDI
BsrFI BsrGI BstEII BstYI Bsu36I BtgI EaeI EarI
EciI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MspA1I
NaeI NcoI NgoMIV NspI PasI PfoI PpiI PpuMI
PspOMI PstI SacII SanDI SapI SexAI SfcI SfiI
SmaI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaAI
BsaBI BsaHI BseRI BsiEI BsiWI BsmI BsmBI BspHI
BspMI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SalI SbfI ScaI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmnI ZraI