Restriction map for
pF1KB8715
(Linear) (Six-base) MAPSORT of: KIBB8715.seq Check: 2502 from: 1 to: 1158
KIBB8715.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 135 570 1158
Size: 135 435 588
Fragments arranged by size:
588 435 135
AhdI GACnn_n'nnGTC
Cuts at: 0 738 1158
Size: 738 420
AlwNI CAG_nnn'CTG
Cuts at: 0 585 888 993 1158
Size: 585 303 105 165
Fragments arranged by size:
585 303 165 105
ApaI G_GGCC'C
Cuts at: 0 16 1158
Size: 16 1142
ApaLI G'TGCA_C
Cuts at: 0 148 1158
Size: 148 1010
AvaI C'yCGr_G
Cuts at: 0 648 1158
Size: 648 510
BanI G'GyrC_C
Cuts at: 0 1060 1158
Size: 1060 98
BanII G_rGCy'C
Cuts at: 0 16 808 1158
Size: 16 792 350
Fragments arranged by size:
792 350 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 758 1158
Size: 758 400
BbvCI CC'TCA_GC
Cuts at: 0 276 1158
Size: 276 882
BciVI GTATCCnnnnn_n'
Cuts at: 0 352 1158
Size: 352 806
BfrBI ATG'CAT
Cuts at: 0 3 1158
Size: 3 1155
BglI GCCn_nnn'nGGC
Cuts at: 0 20 251 385 435 526 633 1158
Size: 20 231 134 50 91 107 525
Fragments arranged by size:
525 231 134 107 91 50 20
BlpI GC'TnA_GC
Cuts at: 0 475 1158
Size: 475 683
Bme1580I G_kGCm'C
Cuts at: 0 16 152 256 426 1096 1158
Size: 16 136 104 170 670 62
Fragments arranged by size:
670 170 136 104 62 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 756 837 1158
Size: 756 81 321
Fragments arranged by size:
756 321 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 825 1158
Size: 825 333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1158
Size: 1015 143
Bpu10I CC'TnA_GC
Cuts at: 0 202 276 1158
Size: 202 74 882
Fragments arranged by size:
882 202 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 1093 1158
Size: 1093 65
BsaHI Gr'CG_yC
Cuts at: 0 596 1158
Size: 596 562
BsaWI w'CCGG_w
Cuts at: 0 548 1158
Size: 548 610
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1073 1103 1158
Size: 1073 30 55
Fragments arranged by size:
1073 55 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 798 963 1158
Size: 42 756 165 195
Fragments arranged by size:
756 195 165 42
BseYI C'CCAG_C
Cuts at: 0 33 388 538 832 1158
Size: 33 355 150 294 326
Fragments arranged by size:
355 326 294 150 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 624 808 1158
Size: 362 262 184 350
Fragments arranged by size:
362 350 262 184
BsiHKAI G_wGCw'C
Cuts at: 0 79 152 1158
Size: 79 73 1006
Fragments arranged by size:
1006 79 73
Bsp1286I G_dGCh'C
Cuts at: 0 16 79 152 256 426 808 1096 1158
Size: 16 63 73 104 170 382 288 62
Fragments arranged by size:
382 288 170 104 73 63 62 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 570 1158
Size: 135 435 588
Fragments arranged by size:
588 435 135
BsrFI r'CCGG_y
Cuts at: 0 97 152 346 520 1158
Size: 97 55 194 174 638
Fragments arranged by size:
638 194 174 97 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 585 888 1158
Size: 156 429 303 270
Fragments arranged by size:
429 303 270 156
BstEII G'GTnAC_C
Cuts at: 0 1107 1158
Size: 1107 51
Bsu36I CC'TnA_GG
Cuts at: 0 631 778 1158
Size: 631 147 380
Fragments arranged by size:
631 380 147
BtgI C'CryG_G
Cuts at: 0 18 417 433 445 949 1113 1158
Size: 18 399 16 12 504 164 45
Fragments arranged by size:
504 399 164 45 18 16 12
BtsI GCAGTG_nn'
Cuts at: 0 37 317 424 469 1158
Size: 37 280 107 45 689
Fragments arranged by size:
689 280 107 45 37
DraIII CAC_nnn'GTG
Cuts at: 0 83 1158
Size: 83 1075
EaeI y'GGCC_r
Cuts at: 0 21 377 1158
Size: 21 356 781
Fragments arranged by size:
781 356 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 676 1158
Size: 676 482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1158
Size: 1015 143
EcoO109I rG'GnC_Cy
Cuts at: 0 12 13 542 608 1144 1158
Size: 12 1 529 66 536 14
Fragments arranged by size:
536 529 66 14 12 1
FseI GG_CCGG'CC
Cuts at: 0 101 1158
Size: 101 1057
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 724 756 1158
Size: 724 32 402
Fragments arranged by size:
724 402 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1059 1158
Size: 1059 99
MscI TGG'CCA
Cuts at: 0 23 379 1158
Size: 23 356 779
Fragments arranged by size:
779 356 23
MspA1I CmG'CkG
Cuts at: 0 353 538 993 1158
Size: 353 185 455 165
Fragments arranged by size:
455 353 185 165
NaeI GCC'GGC
Cuts at: 0 99 348 522 1158
Size: 99 249 174 636
Fragments arranged by size:
636 249 174 99
NcoI C'CATG_G
Cuts at: 0 18 949 1113 1158
Size: 18 931 164 45
Fragments arranged by size:
931 164 45 18
NgoMIV G'CCGG_C
Cuts at: 0 97 346 520 1158
Size: 97 249 174 638
Fragments arranged by size:
638 249 174 97
NsiI A_TGCA'T
Cuts at: 0 5 1158
Size: 5 1153
NspI r_CATG'y
Cuts at: 0 7 274 366 1158
Size: 7 267 92 792
Fragments arranged by size:
792 267 92 7
PasI CC'CwG_GG
Cuts at: 0 171 850 1158
Size: 171 679 308
Fragments arranged by size:
679 308 171
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 1158
Size: 171 987
PpuMI rG'GwC_Cy
Cuts at: 0 608 1144 1158
Size: 608 536 14
Fragments arranged by size:
608 536 14
PspOMI G'GGCC_C
Cuts at: 0 12 1158
Size: 12 1146
PstI C_TGCA'G
Cuts at: 0 500 581 590 893 989 1158
Size: 500 81 9 303 96 169
Fragments arranged by size:
500 303 169 96 81 9
PvuII CAG'CTG
Cuts at: 0 353 538 993 1158
Size: 353 185 455 165
Fragments arranged by size:
455 353 185 165
SfcI C'TryA_G
Cuts at: 0 496 577 586 889 985 1158
Size: 496 81 9 303 96 173
Fragments arranged by size:
496 303 173 96 81 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 20 1158
Size: 20 1138
SmaI CCC'GGG
Cuts at: 0 650 1158
Size: 650 508
SphI G_CATG'C
Cuts at: 0 7 274 1158
Size: 7 267 884
Fragments arranged by size:
884 267 7
StuI AGG'CCT
Cuts at: 0 194 326 696 783 821 907 1158
Size: 194 132 370 87 38 86 251
Fragments arranged by size:
370 251 194 132 87 86 38
StyI C'CwwG_G
Cuts at: 0 18 327 611 872 949 1113 1158
Size: 18 309 284 261 77 164 45
Fragments arranged by size:
309 284 261 164 77 45 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 691 1158
Size: 691 467
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1073 1105 1158
Size: 1073 32 53
Fragments arranged by size:
1073 53 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1050 1120 1158
Size: 1050 70 38
Fragments arranged by size:
1050 70 38
XmaI C'CCGG_G
Cuts at: 0 648 1158
Size: 648 510
Enzymes that do cut:
AarI AhdI AlwNI ApaI ApaLI AvaI BanI BanII
BbsI BbvCI BciVI BfrBI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstEII
Bsu36I BtgI BtsI DraIII EaeI EciI Eco57MI EcoO109I
FseI Hin4I MmeI MscI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PflMI PpuMI PspOMI PstI PvuII
SfcI SfiI SmaI SphI StuI StyI TaqII TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BclI BglII BmgBI
BmtI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MslI NarI NdeI NheI NotI NruI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmnI ZraI