Restriction map for
pF1KB8717
(Linear) (Six-base) MAPSORT of: KIBB8717.seq Check: 3313 from: 1 to: 1443
KIBB8717.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 920 1443
Size: 920 523
AccI GT'mk_AC
Cuts at: 0 633 1443
Size: 633 810
AclI AA'CG_TT
Cuts at: 0 377 1443
Size: 377 1066
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 805 1443
Size: 459 346 638
Fragments arranged by size:
638 459 346
AflII C'TTAA_G
Cuts at: 0 267 1443
Size: 267 1176
AhdI GACnn_n'nnGTC
Cuts at: 0 396 1443
Size: 396 1047
AleI CACnn'nnGTG
Cuts at: 0 1186 1443
Size: 1186 257
AlwNI CAG_nnn'CTG
Cuts at: 0 195 1113 1443
Size: 195 918 330
Fragments arranged by size:
918 330 195
AvaI C'yCGr_G
Cuts at: 0 666 1184 1297 1443
Size: 666 518 113 146
Fragments arranged by size:
666 518 146 113
BamHI G'GATC_C
Cuts at: 0 807 1443
Size: 807 636
BanII G_rGCy'C
Cuts at: 0 1406 1443
Size: 1406 37
BbvCI CC'TCA_GC
Cuts at: 0 1397 1443
Size: 1397 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 111 145 1443
Size: 111 34 1298
Fragments arranged by size:
1298 111 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 187 1003 1443
Size: 187 816 440
Fragments arranged by size:
816 440 187
BfrBI ATG'CAT
Cuts at: 0 733 1443
Size: 733 710
BglII A'GATC_T
Cuts at: 0 210 1443
Size: 210 1233
BmgBI CAC'GTC
Cuts at: 0 66 1443
Size: 66 1377
BmrI ACTGGGnnnn_n'
Cuts at: 0 1412 1443
Size: 1412 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1422 1443
Size: 1422 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 205 907 1197 1443
Size: 42 163 702 290 246
Fragments arranged by size:
702 290 246 163 42
Bpu10I CC'TnA_GC
Cuts at: 0 85 1262 1397 1443
Size: 85 1177 135 46
Fragments arranged by size:
1177 135 85 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1443
Size: 676 767
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 1309 1443
Size: 295 1014 134
Fragments arranged by size:
1014 295 134
BsaHI Gr'CG_yC
Cuts at: 0 164 1443
Size: 164 1279
BsaWI w'CCGG_w
Cuts at: 0 172 1443
Size: 172 1271
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 515 1443
Size: 97 418 928
Fragments arranged by size:
928 418 97
BseYI C'CCAG_C
Cuts at: 0 699 773 826 830 1443
Size: 699 74 53 4 613
Fragments arranged by size:
699 613 74 53 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1443
Size: 627 816
BsiEI CG_ry'CG
Cuts at: 0 143 1443
Size: 143 1300
BsiHKAI G_wGCw'C
Cuts at: 0 95 1443
Size: 95 1348
BsmI GAATG_Cn'
Cuts at: 0 733 1443
Size: 733 710
BsmBI CGTCTCn'nnnn_
Cuts at: 0 922 1443
Size: 922 521
Bsp1286I G_dGCh'C
Cuts at: 0 95 1406 1443
Size: 95 1311 37
Fragments arranged by size:
1311 95 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 920 1395 1443
Size: 189 731 475 48
Fragments arranged by size:
731 475 189 48
BsrDI GCAATG_nn'
Cuts at: 0 1094 1443
Size: 1094 349
BssHII G'CGCG_C
Cuts at: 0 118 1443
Size: 118 1325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 195 1025 1443
Size: 195 830 418
Fragments arranged by size:
830 418 195
BstEII G'GTnAC_C
Cuts at: 0 1164 1443
Size: 1164 279
BstYI r'GATC_y
Cuts at: 0 210 687 807 1443
Size: 210 477 120 636
Fragments arranged by size:
636 477 210 120
Bsu36I CC'TnA_GG
Cuts at: 0 812 1348 1443
Size: 812 536 95
Fragments arranged by size:
812 536 95
BtgI C'CryG_G
Cuts at: 0 1336 1443
Size: 1336 107
BtsI GCAGTG_nn'
Cuts at: 0 153 1165 1443
Size: 153 1012 278
Fragments arranged by size:
1012 278 153
DraIII CAC_nnn'GTG
Cuts at: 0 985 1443
Size: 985 458
EaeI y'GGCC_r
Cuts at: 0 140 308 1443
Size: 140 168 1135
Fragments arranged by size:
1135 168 140
EagI C'GGCC_G
Cuts at: 0 140 1443
Size: 140 1303
EarI CTCTTCn'nnn_
Cuts at: 0 743 1429 1443
Size: 743 686 14
Fragments arranged by size:
743 686 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 205 459 805 907 1197 1443
Size: 42 163 254 346 102 290 246
Fragments arranged by size:
346 290 254 246 163 102 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 283 1443
Size: 283 1160
EcoO109I rG'GnC_Cy
Cuts at: 0 201 770 1217 1443
Size: 201 569 447 226
Fragments arranged by size:
569 447 226 201
HaeII r_GCGC'y
Cuts at: 0 137 1443
Size: 137 1306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 275 307 382 414 662 694 943 975
Size: 275 32 75 32 248 32 249 32
Cuts at: 975 1443
Size: 468
Fragments arranged by size:
468 275 249 248 75 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 306 1443
Size: 214 92 1137
Fragments arranged by size:
1137 214 92
MslI CAynn'nnrTG
Cuts at: 0 547 1186 1443
Size: 547 639 257
Fragments arranged by size:
639 547 257
MspA1I CmG'CkG
Cuts at: 0 80 183 830 1338 1443
Size: 80 103 647 508 105
Fragments arranged by size:
647 508 105 103 80
NsiI A_TGCA'T
Cuts at: 0 735 1443
Size: 735 708
NspI r_CATG'y
Cuts at: 0 126 1443
Size: 126 1317
PasI CC'CwG_GG
Cuts at: 0 932 1443
Size: 932 511
PflMI CCAn_nnn'nTGG
Cuts at: 0 1113 1443
Size: 1113 330
PfoI T'CCnGG_A
Cuts at: 0 947 1443
Size: 947 496
PpuMI rG'GwC_Cy
Cuts at: 0 201 1217 1443
Size: 201 1016 226
Fragments arranged by size:
1016 226 201
PspXI vC'TCGA_Gb
Cuts at: 0 1184 1443
Size: 1184 259
PstI C_TGCA'G
Cuts at: 0 200 1443
Size: 200 1243
PvuII CAG'CTG
Cuts at: 0 830 1443
Size: 830 613
SacII CC_GC'GG
Cuts at: 0 1339 1443
Size: 1339 104
SfcI C'TryA_G
Cuts at: 0 196 450 974 1443
Size: 196 254 524 469
Fragments arranged by size:
524 469 254 196
SmlI C'TyrA_G
Cuts at: 0 267 666 691 1184 1443
Size: 267 399 25 493 259
Fragments arranged by size:
493 399 267 259 25
StuI AGG'CCT
Cuts at: 0 782 1246 1443
Size: 782 464 197
Fragments arranged by size:
782 464 197
StyI C'CwwG_G
Cuts at: 0 422 872 1443
Size: 422 450 571
Fragments arranged by size:
571 450 422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 386 1152 1443
Size: 386 766 291
Fragments arranged by size:
766 386 291
Tth111I GACn'n_nGTC
Cuts at: 0 287 409 1144 1443
Size: 287 122 735 299
Fragments arranged by size:
735 299 287 122
XhoI C'TCGA_G
Cuts at: 0 666 1184 1443
Size: 666 518 259
Fragments arranged by size:
666 518 259
XmnI GAAnn'nnTTC
Cuts at: 0 377 1366 1443
Size: 377 989 77
Fragments arranged by size:
989 377 77
Enzymes that do cut:
AarI AccI AclI AcuI AflII AhdI AleI AlwNI
AvaI BamHI BanII BbvCI BcgI BciVI BfrBI BglII
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BssHII BstAPI BstEII BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MslI MspA1I NsiI NspI
PasI PflMI PfoI PpuMI PspXI PstI PvuII SacII
SfcI SmlI StuI StyI TaqII Tth111I XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflIII AgeI AlfI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbsI BclI BglI BlpI Bme1580I BmtI BsaAI
BsaBI BsaXI BsiWI BspEI BspHI BsrBI BsrFI BsrGI
BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI
DrdI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
XbaI XcmI XmaI ZraI