Restriction map for
pF1KB8718
(Linear) (Six-base) MAPSORT of: KIBB8718.seq Check: 6604 from: 1 to: 1815
KIBB8718.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 595 1815
Size: 595 1220
AatII G_ACGT'C
Cuts at: 0 1265 1601 1815
Size: 1265 336 214
Fragments arranged by size:
1265 336 214
AccI GT'mk_AC
Cuts at: 0 450 1815
Size: 450 1365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 1240 1255 1648 1815
Size: 1086 154 15 393 167
Fragments arranged by size:
1086 393 167 154 15
AflIII A'CryG_T
Cuts at: 0 938 1496 1787 1815
Size: 938 558 291 28
Fragments arranged by size:
938 558 291 28
AleI CACnn'nnGTG
Cuts at: 0 586 1815
Size: 586 1229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 604 805 895 1267 1579 1815
Size: 604 201 90 372 312 236
Fragments arranged by size:
604 372 312 236 201 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 925 957 1815
Size: 925 32 858
Fragments arranged by size:
925 858 32
ApaLI G'TGCA_C
Cuts at: 0 672 1815
Size: 672 1143
AvaI C'yCGr_G
Cuts at: 0 110 232 989 1334 1815
Size: 110 122 757 345 481
Fragments arranged by size:
757 481 345 122 110
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 561 594 1815
Size: 561 33 1221
Fragments arranged by size:
1221 561 33
BamHI G'GATC_C
Cuts at: 0 1810 1815
Size: 1810 5
BanI G'GyrC_C
Cuts at: 0 394 686 885 1815
Size: 394 292 199 930
Fragments arranged by size:
930 394 292 199
BanII G_rGCy'C
Cuts at: 0 38 1815
Size: 38 1777
BbeI G_GCGC'C
Cuts at: 0 398 1815
Size: 398 1417
BbvCI CC'TCA_GC
Cuts at: 0 1712 1815
Size: 1712 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 987 1021 1230 1264 1815
Size: 987 34 209 34 551
Fragments arranged by size:
987 551 209 34 34
BlpI GC'TnA_GC
Cuts at: 0 712 1815
Size: 712 1103
Bme1580I G_kGCm'C
Cuts at: 0 676 1137 1292 1532 1815
Size: 676 461 155 240 283
Fragments arranged by size:
676 461 283 240 155
BmgBI CAC'GTC
Cuts at: 0 222 822 1815
Size: 222 600 993
Fragments arranged by size:
993 600 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 425 1815
Size: 425 1390
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 669 1815
Size: 669 1146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 883 1108 1443 1639 1815
Size: 445 438 225 335 196 176
Fragments arranged by size:
445 438 335 225 196 176
Bpu10I CC'TnA_GC
Cuts at: 0 737 803 1472 1712 1815
Size: 737 66 669 240 103
Fragments arranged by size:
737 669 240 103 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1815
Size: 22 1793
BsaBI GATnn'nnATC
Cuts at: 0 968 1815
Size: 968 847
BsaHI Gr'CG_yC
Cuts at: 0 395 955 1096 1262 1598 1815
Size: 395 560 141 166 336 217
Fragments arranged by size:
560 395 336 217 166 141
BsaWI w'CCGG_w
Cuts at: 0 659 950 1739 1815
Size: 659 291 789 76
Fragments arranged by size:
789 659 291 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 148 178 472 502 925 955
Size: 70 30 48 30 294 30 423 30
Cuts at: 955 1615 1645 1815
Size: 660 30 170
Fragments arranged by size:
660 423 294 170 70 48 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 169 1105 1636 1815
Size: 94 75 936 531 179
Fragments arranged by size:
936 531 179 94 75
BseYI C'CCAG_C
Cuts at: 0 184 261 266 565 1815
Size: 184 77 5 299 1250
Fragments arranged by size:
1250 299 184 77 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 629 647 1304 1753 1815
Size: 623 6 18 657 449 62
Fragments arranged by size:
657 623 449 62 18 6
BsiEI CG_ry'CG
Cuts at: 0 660 702 1815
Size: 660 42 1113
Fragments arranged by size:
1113 660 42
BsiHKAI G_wGCw'C
Cuts at: 0 38 593 676 794 1525 1815
Size: 38 555 83 118 731 290
Fragments arranged by size:
731 555 290 118 83 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1087 1815
Size: 1087 728
Bsp1286I G_dGCh'C
Cuts at: 0 38 593 676 794 1137 1292 1525 1532
Size: 38 555 83 118 343 155 233 7
Cuts at: 1532 1815
Size: 283
Fragments arranged by size:
555 343 283 233 155 118 83 38
7
BspEI T'CCGG_A
Cuts at: 0 950 1739 1815
Size: 950 789 76
Fragments arranged by size:
950 789 76
BspHI T'CATG_A
Cuts at: 0 1139 1815
Size: 1139 676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 595 1815
Size: 595 1220
BsrDI GCAATG_nn'
Cuts at: 0 132 1815
Size: 132 1683
BsrFI r'CCGG_y
Cuts at: 0 683 1018 1815
Size: 683 335 797
Fragments arranged by size:
797 683 335
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 127 540 975 1815
Size: 30 97 413 435 840
Fragments arranged by size:
840 435 413 97 30
BstYI r'GATC_y
Cuts at: 0 1614 1810 1815
Size: 1614 196 5
Fragments arranged by size:
1614 196 5
Bsu36I CC'TnA_GG
Cuts at: 0 191 751 1306 1725 1815
Size: 191 560 555 419 90
Fragments arranged by size:
560 555 419 191 90
BtgI C'CryG_G
Cuts at: 0 366 1670 1815
Size: 366 1304 145
Fragments arranged by size:
1304 366 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1412 1815
Size: 1412 403
BtsI GCAGTG_nn'
Cuts at: 0 634 1292 1815
Size: 634 658 523
Fragments arranged by size:
658 634 523
DraIII CAC_nnn'GTG
Cuts at: 0 1071 1815
Size: 1071 744
DrdI GACnn_nn'nnGTC
Cuts at: 0 664 1188 1815
Size: 664 524 627
Fragments arranged by size:
664 627 524
EaeI y'GGCC_r
Cuts at: 0 89 240 542 1040 1673 1815
Size: 89 151 302 498 633 142
Fragments arranged by size:
633 498 302 151 142 89
EarI CTCTTCn'nnn_
Cuts at: 0 1231 1815
Size: 1231 584
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1528 1815
Size: 1528 287
EcoICRI GAG'CTC
Cuts at: 0 36 1815
Size: 36 1779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 883 1086 1108 1240 1255 1443 1639
Size: 445 438 203 22 132 15 188 196
Cuts at: 1639 1648 1815
Size: 9 167
Fragments arranged by size:
445 438 203 196 188 167 132 22
15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1104 1815
Size: 1104 711
EcoO109I rG'GnC_Cy
Cuts at: 0 228 420 510 1815
Size: 228 192 90 1305
Fragments arranged by size:
1305 228 192 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 870 1815
Size: 870 945
HaeII r_GCGC'y
Cuts at: 0 162 398 1815
Size: 162 236 1417
Fragments arranged by size:
1417 236 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 148 180 1328 1360 1815
Size: 70 32 46 32 1148 32 455
Fragments arranged by size:
1148 455 70 46 32 32 32
HincII GTy'rAC
Cuts at: 0 1353 1815
Size: 1353 462
KasI G'GCGC_C
Cuts at: 0 394 1815
Size: 394 1421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1815
Size: 680 1135
MscI TGG'CCA
Cuts at: 0 242 544 1675 1815
Size: 242 302 1131 140
Fragments arranged by size:
1131 302 242 140
MslI CAynn'nnrTG
Cuts at: 0 586 787 1144 1815
Size: 586 201 357 671
Fragments arranged by size:
671 586 357 201
MspA1I CmG'CkG
Cuts at: 0 519 613 744 1393 1815
Size: 519 94 131 649 422
Fragments arranged by size:
649 519 422 131 94
NaeI GCC'GGC
Cuts at: 0 1020 1815
Size: 1020 795
NarI GG'CG_CC
Cuts at: 0 395 1815
Size: 395 1420
NgoMIV G'CCGG_C
Cuts at: 0 1018 1815
Size: 1018 797
NspI r_CATG'y
Cuts at: 0 1179 1500 1791 1815
Size: 1179 321 291 24
Fragments arranged by size:
1179 321 291 24
PasI CC'CwG_GG
Cuts at: 0 312 1815
Size: 312 1503
PciI A'CATG_T
Cuts at: 0 1496 1787 1815
Size: 1496 291 28
Fragments arranged by size:
1496 291 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 565 1815
Size: 312 253 1250
Fragments arranged by size:
1250 312 253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 925 957 1815
Size: 925 32 858
Fragments arranged by size:
925 858 32
PpuMI rG'GwC_Cy
Cuts at: 0 228 420 1815
Size: 228 192 1395
Fragments arranged by size:
1395 228 192
PshAI GACnn'nnGTC
Cuts at: 0 1372 1815
Size: 1372 443
PstI C_TGCA'G
Cuts at: 0 524 1815
Size: 524 1291
PvuII CAG'CTG
Cuts at: 0 519 613 744 1393 1815
Size: 519 94 131 649 422
Fragments arranged by size:
649 519 422 131 94
SacI G_AGCT'C
Cuts at: 0 38 1815
Size: 38 1777
SapI GCTCTTCn'nnn_
Cuts at: 0 1231 1815
Size: 1231 584
SfcI C'TryA_G
Cuts at: 0 514 520 1815
Size: 514 6 1295
Fragments arranged by size:
1295 514 6
SfoI GGC'GCC
Cuts at: 0 396 1815
Size: 396 1419
SmaI CCC'GGG
Cuts at: 0 112 1815
Size: 112 1703
SmlI C'TyrA_G
Cuts at: 0 37 232 1815
Size: 37 195 1583
Fragments arranged by size:
1583 195 37
StyI C'CwwG_G
Cuts at: 0 533 758 1703 1815
Size: 533 225 945 112
Fragments arranged by size:
945 533 225 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 1815
Size: 589 1226
TatI w'GTAC_w
Cuts at: 0 866 1815
Size: 866 949
Tth111I GACn'n_nGTC
Cuts at: 0 1597 1815
Size: 1597 218
XhoI C'TCGA_G
Cuts at: 0 232 1815
Size: 232 1583
XmaI C'CCGG_G
Cuts at: 0 110 1815
Size: 110 1705
XmnI GAAnn'nnTTC
Cuts at: 0 940 1815
Size: 940 875
ZraI GAC'GTC
Cuts at: 0 1263 1599 1815
Size: 1263 336 216
Fragments arranged by size:
1263 336 216
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
ApaLI AvaI BaeI BamHI BanI BanII BbeI BbvCI
BcgI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PpiI PpuMI PshAI PstI PvuII SacI SapI SfcI
SfoI SmaI SmlI StyI TaqII TatI Tth111I XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlwNI ApaI
ApoI AscI AseI AsiSI AvrII BbsI BciVI BclI
BfrBI BglI BglII BmtI BsaI BsaAI BsiWI BsmI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
ClaI CspCI DraI EagI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XcmI