Restriction map for
pF1KB8720
(Linear) (Six-base) MAPSORT of: KIBB8720.seq Check: 3088 from: 1 to: 2160
KIBB8720.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 898 2160
Size: 341 557 1262
Fragments arranged by size:
1262 557 341
AflIII A'CryG_T
Cuts at: 0 1977 2160
Size: 1977 183
AgeI A'CCGG_T
Cuts at: 0 2064 2160
Size: 2064 96
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2125 2157 2160
Size: 2125 32 3
Fragments arranged by size:
2125 32 3
AlwNI CAG_nnn'CTG
Cuts at: 0 1108 1233 1918 2160
Size: 1108 125 685 242
Fragments arranged by size:
1108 685 242 125
ApaI G_GGCC'C
Cuts at: 0 470 2160
Size: 470 1690
ApaLI G'TGCA_C
Cuts at: 0 969 1431 2160
Size: 969 462 729
Fragments arranged by size:
969 729 462
ApoI r'AATT_y
Cuts at: 0 46 1006 1311 1355 2160
Size: 46 960 305 44 805
Fragments arranged by size:
960 805 305 46 44
AvaI C'yCGr_G
Cuts at: 0 542 634 656 795 936 1826 2160
Size: 542 92 22 139 141 890 334
Fragments arranged by size:
890 542 334 141 139 92 22
BanI G'GyrC_C
Cuts at: 0 523 546 1176 2160
Size: 523 23 630 984
Fragments arranged by size:
984 630 523 23
BanII G_rGCy'C
Cuts at: 0 217 470 1606 1646 2095 2116 2160
Size: 217 253 1136 40 449 21 44
Fragments arranged by size:
1136 449 253 217 44 40 21
BbeI G_GCGC'C
Cuts at: 0 550 2160
Size: 550 1610
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 62 93 329 889 2160
Size: 26 36 31 236 560 1271
Fragments arranged by size:
1271 560 236 36 31 26
BbvCI CC'TCA_GC
Cuts at: 0 1185 2160
Size: 1185 975
BglI GCCn_nnn'nGGC
Cuts at: 0 833 2160
Size: 833 1327
BlpI GC'TnA_GC
Cuts at: 0 1507 2110 2160
Size: 1507 603 50
Fragments arranged by size:
1507 603 50
Bme1580I G_kGCm'C
Cuts at: 0 470 528 973 1416 1435 2160
Size: 470 58 445 443 19 725
Fragments arranged by size:
725 470 445 443 58 19
BmgBI CAC'GTC
Cuts at: 0 419 1436 2160
Size: 419 1017 724
Fragments arranged by size:
1017 724 419
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2114 2160
Size: 2114 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 1224 1760 1895 2015 2160
Size: 231 993 536 135 120 145
Fragments arranged by size:
993 536 231 145 135 120
Bpu10I CC'TnA_GC
Cuts at: 0 1185 2089 2160
Size: 1185 904 71
Fragments arranged by size:
1185 904 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2137 2160
Size: 2137 23
BsaAI yAC'GTr
Cuts at: 0 1980 2160
Size: 1980 180
BsaHI Gr'CG_yC
Cuts at: 0 547 2160
Size: 547 1613
BsaWI w'CCGG_w
Cuts at: 0 162 1212 2064 2160
Size: 162 1050 852 96
Fragments arranged by size:
1050 852 162 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2013 2043 2077 2107 2160
Size: 2013 30 34 30 53
Fragments arranged by size:
2013 53 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 489 924 1730 2140 2160
Size: 489 435 806 410 20
Fragments arranged by size:
806 489 435 410 20
BseYI C'CCAG_C
Cuts at: 0 462 514 747 978 2160
Size: 462 52 233 231 1182
Fragments arranged by size:
1182 462 233 231 52
BsiEI CG_ry'CG
Cuts at: 0 818 1423 2160
Size: 818 605 737
Fragments arranged by size:
818 737 605
BsiHKAI G_wGCw'C
Cuts at: 0 973 1435 2095 2160
Size: 973 462 660 65
Fragments arranged by size:
973 660 462 65
BsmBI CGTCTCn'nnnn_
Cuts at: 0 425 1623 2160
Size: 425 1198 537
Fragments arranged by size:
1198 537 425
Bsp1286I G_dGCh'C
Cuts at: 0 217 470 528 973 1416 1435 1606 1646
Size: 217 253 58 445 443 19 171 40
Cuts at: 1646 2095 2116 2160
Size: 449 21 44
Fragments arranged by size:
449 445 443 253 217 171 58 44
40 21 19
BspEI T'CCGG_A
Cuts at: 0 1212 2160
Size: 1212 948
BspHI T'CATG_A
Cuts at: 0 2018 2160
Size: 2018 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 798 2160
Size: 798 1362
BsrBI CCG'CTC
Cuts at: 0 661 953 2160
Size: 661 292 1207
Fragments arranged by size:
1207 661 292
BsrDI GCAATG_nn'
Cuts at: 0 1667 2160
Size: 1667 493
BsrFI r'CCGG_y
Cuts at: 0 813 1547 2064 2160
Size: 813 734 517 96
Fragments arranged by size:
813 734 517 96
BsrGI T'GTAC_A
Cuts at: 0 245 2160
Size: 245 1915
BstAPI GCAn_nnn'nTGC
Cuts at: 0 964 2160
Size: 964 1196
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1599 1713 2160
Size: 1599 114 447
Fragments arranged by size:
1599 447 114
BstYI r'GATC_y
Cuts at: 0 1990 2160
Size: 1990 170
Bsu36I CC'TnA_GG
Cuts at: 0 1757 2117 2160
Size: 1757 360 43
Fragments arranged by size:
1757 360 43
BtgI C'CryG_G
Cuts at: 0 1598 2160
Size: 1598 562
BtsI GCAGTG_nn'
Cuts at: 0 593 989 2160
Size: 593 396 1171
Fragments arranged by size:
1171 593 396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1827 1862 2160
Size: 1827 35 298
Fragments arranged by size:
1827 298 35
DraI TTT'AAA
Cuts at: 0 1353 2160
Size: 1353 807
DraIII CAC_nnn'GTG
Cuts at: 0 1519 2160
Size: 1519 641
EaeI y'GGCC_r
Cuts at: 0 35 221 815 1087 1224 1420 2051 2160
Size: 35 186 594 272 137 196 631 109
Fragments arranged by size:
631 594 272 196 186 137 109 35
EagI C'GGCC_G
Cuts at: 0 815 1420 2160
Size: 815 605 740
Fragments arranged by size:
815 740 605
EarI CTCTTCn'nnn_
Cuts at: 0 1877 2160
Size: 1877 283
EcoICRI GAG'CTC
Cuts at: 0 2093 2160
Size: 2093 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 341 898 1224 1760 1895 2015 2160
Size: 231 110 557 326 536 135 120 145
Fragments arranged by size:
557 536 326 231 145 135 120 110
EcoO109I rG'GnC_Cy
Cuts at: 0 466 467 553 940 1072 1105 2160
Size: 466 1 86 387 132 33 1055
Fragments arranged by size:
1055 466 387 132 86 33 1
EcoRV GAT'ATC
Cuts at: 0 1884 2160
Size: 1884 276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 43 2160
Size: 43 2117
HaeII r_GCGC'y
Cuts at: 0 550 1554 2160
Size: 550 1004 606
Fragments arranged by size:
1004 606 550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 745 777 2013 2045 2077 2109 2160
Size: 745 32 1236 32 32 32 51
Fragments arranged by size:
1236 745 51 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1402 2160
Size: 1402 758
KasI G'GCGC_C
Cuts at: 0 546 2160
Size: 546 1614
MfeI C'AATT_G
Cuts at: 0 1267 2160
Size: 1267 893
MscI TGG'CCA
Cuts at: 0 2053 2160
Size: 2053 107
MslI CAynn'nnrTG
Cuts at: 0 770 2160
Size: 770 1390
MspA1I CmG'CkG
Cuts at: 0 751 1162 1419 2109 2160
Size: 751 411 257 690 51
Fragments arranged by size:
751 690 411 257 51
NaeI GCC'GGC
Cuts at: 0 815 1549 2160
Size: 815 734 611
Fragments arranged by size:
815 734 611
NarI GG'CG_CC
Cuts at: 0 547 2160
Size: 547 1613
NgoMIV G'CCGG_C
Cuts at: 0 813 1547 2160
Size: 813 734 613
Fragments arranged by size:
813 734 613
NotI GC'GGCC_GC
Cuts at: 0 1420 2160
Size: 1420 740
PasI CC'CwG_GG
Cuts at: 0 581 688 1592 2160
Size: 581 107 904 568
Fragments arranged by size:
904 581 568 107
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 2160
Size: 342 1818
PfoI T'CCnGG_A
Cuts at: 0 733 2160
Size: 733 1427
PmlI CAC'GTG
Cuts at: 0 1980 2160
Size: 1980 180
PpuMI rG'GwC_Cy
Cuts at: 0 1072 2160
Size: 1072 1088
PspOMI G'GGCC_C
Cuts at: 0 466 2160
Size: 466 1694
PspXI vC'TCGA_Gb
Cuts at: 0 656 2160
Size: 656 1504
PstI C_TGCA'G
Cuts at: 0 1104 2160
Size: 1104 1056
PvuII CAG'CTG
Cuts at: 0 751 2109 2160
Size: 751 1358 51
Fragments arranged by size:
1358 751 51
SacI G_AGCT'C
Cuts at: 0 2095 2160
Size: 2095 65
SanDI GG'GwC_CC
Cuts at: 0 1072 2160
Size: 1072 1088
SapI GCTCTTCn'nnn_
Cuts at: 0 1877 2160
Size: 1877 283
SbfI CC_TGCA'GG
Cuts at: 0 1104 2160
Size: 1104 1056
ScaI AGT'ACT
Cuts at: 0 1809 2160
Size: 1809 351
SfcI C'TryA_G
Cuts at: 0 1100 1985 2160
Size: 1100 885 175
Fragments arranged by size:
1100 885 175
SfoI GGC'GCC
Cuts at: 0 548 2160
Size: 548 1612
SgrAI Cr'CCGG_yG
Cuts at: 0 2064 2160
Size: 2064 96
SmaI CCC'GGG
Cuts at: 0 544 636 797 2160
Size: 544 92 161 1363
Fragments arranged by size:
1363 544 161 92
SmlI C'TyrA_G
Cuts at: 0 656 2152 2160
Size: 656 1496 8
Fragments arranged by size:
1496 656 8
StuI AGG'CCT
Cuts at: 0 2004 2160
Size: 2004 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 466 1213 2160
Size: 466 747 947
Fragments arranged by size:
947 747 466
TatI w'GTAC_w
Cuts at: 0 245 1807 2160
Size: 245 1562 353
Fragments arranged by size:
1562 353 245
XhoI C'TCGA_G
Cuts at: 0 656 2160
Size: 656 1504
XmaI C'CCGG_G
Cuts at: 0 542 634 795 2160
Size: 542 92 161 1365
Fragments arranged by size:
1365 542 161 92
XmnI GAAnn'nnTTC
Cuts at: 0 23 2160
Size: 23 2137
Enzymes that do cut:
AcuI AflIII AgeI AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI
HaeII Hin4I HindIII KasI MfeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI PasI PflMI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI
SbfI ScaI SfcI SfoI SgrAI SmaI SmlI StuI
TaqII TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BglII BmrI BmtI BsaI
BsaBI BsgI BsiWI BsmI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI DrdI EciI EcoNI EcoRI FseI
FspI FspAI HincII HpaI KpnI MluI MmeI NcoI
NdeI NheI NruI NsiI NspI PacI PciI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI
StyI SwaI TstI Tth111I XbaI XcmI ZraI