Restriction map for
pF1KB8721
(Linear) (Six-base) MAPSORT of: KIBB8721.seq Check: 9506 from: 1 to: 1671
KIBB8721.orf
With 182 enzymes: SgfI *
July 24, 2008 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 104 1119 1671
Size: 104 1015 552
Fragments arranged by size:
1015 552 104
AccI GT'mk_AC
Cuts at: 0 896 1175 1671
Size: 896 279 496
Fragments arranged by size:
896 496 279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 388 1354 1466 1671
Size: 2 386 966 112 205
Fragments arranged by size:
966 386 205 112 2
AleI CACnn'nnGTG
Cuts at: 0 1415 1671
Size: 1415 256
AlwNI CAG_nnn'CTG
Cuts at: 0 42 126 366 1062 1152 1234 1522 1671
Size: 42 84 240 696 90 82 288 149
Fragments arranged by size:
696 288 240 149 90 84 82 42
ApaI G_GGCC'C
Cuts at: 0 237 492 1298 1332 1671
Size: 237 255 806 34 339
Fragments arranged by size:
806 339 255 237 34
ApaLI G'TGCA_C
Cuts at: 0 204 1408 1671
Size: 204 1204 263
Fragments arranged by size:
1204 263 204
AseI AT'TA_AT
Cuts at: 0 869 1671
Size: 869 802
AvaI C'yCGr_G
Cuts at: 0 66 625 657 1671
Size: 66 559 32 1014
Fragments arranged by size:
1014 559 66 32
BanI G'GyrC_C
Cuts at: 0 712 1671
Size: 712 959
BanII G_rGCy'C
Cuts at: 0 67 92 237 492 1298 1332 1671
Size: 67 25 145 255 806 34 339
Fragments arranged by size:
806 339 255 145 67 34 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1621 1671
Size: 1621 50
BbvCI CC'TCA_GC
Cuts at: 0 1523 1671
Size: 1523 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 300 334 1671
Size: 300 34 1337
Fragments arranged by size:
1337 300 34
BglI GCCn_nnn'nGGC
Cuts at: 0 39 161 565 721 1302 1671
Size: 39 122 404 156 581 369
Fragments arranged by size:
581 404 369 156 122 39
BglII A'GATC_T
Cuts at: 0 1592 1671
Size: 1592 79
BlpI GC'TnA_GC
Cuts at: 0 100 1371 1671
Size: 100 1271 300
Fragments arranged by size:
1271 300 100
Bme1580I G_kGCm'C
Cuts at: 0 208 237 492 1298 1332 1412 1671
Size: 208 29 255 806 34 80 259
Fragments arranged by size:
806 259 255 208 80 34 29
BmgBI CAC'GTC
Cuts at: 0 1386 1671
Size: 1386 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 1292 1671
Size: 1292 379
BmtI G_CTAG'C
Cuts at: 0 856 1671
Size: 856 815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 259 265 658 1671
Size: 46 213 6 393 1013
Fragments arranged by size:
1013 393 213 46 6
Bpu10I CC'TnA_GC
Cuts at: 0 599 1523 1671
Size: 599 924 148
Fragments arranged by size:
924 599 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 898 959 1671
Size: 898 61 712
Fragments arranged by size:
898 712 61
BsaBI GATnn'nnATC
Cuts at: 0 964 1671
Size: 964 707
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 185 215 1671
Size: 185 30 1456
Fragments arranged by size:
1456 185 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 84 97 100 256 643 916 1671
Size: 84 13 3 156 387 273 755
Fragments arranged by size:
755 387 273 156 84 13 3
BseYI C'CCAG_C
Cuts at: 0 35 252 563 1062 1234 1671
Size: 35 217 311 499 172 437
Fragments arranged by size:
499 437 311 217 172 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 1671
Size: 1181 490
BsiEI CG_ry'CG
Cuts at: 0 165 1671
Size: 165 1506
BsiHKAI G_wGCw'C
Cuts at: 0 208 251 1412 1671
Size: 208 43 1161 259
Fragments arranged by size:
1161 259 208 43
BsmI GAATG_Cn'
Cuts at: 0 1309 1368 1671
Size: 1309 59 303
Fragments arranged by size:
1309 303 59
Bsp1286I G_dGCh'C
Cuts at: 0 67 92 208 237 251 492 1298 1332
Size: 67 25 116 29 14 241 806 34
Cuts at: 1332 1412 1671
Size: 80 259
Fragments arranged by size:
806 259 241 116 80 67 34 29
25 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 1119 1671
Size: 104 1015 552
Fragments arranged by size:
1015 552 104
BsrFI r'CCGG_y
Cuts at: 0 164 373 1671
Size: 164 209 1298
Fragments arranged by size:
1298 209 164
BsrGI T'GTAC_A
Cuts at: 0 1099 1671
Size: 1099 572
BssHII G'CGCG_C
Cuts at: 0 617 1671
Size: 617 1054
BssSI C'ACGA_G
Cuts at: 0 1540 1671
Size: 1540 131
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1363 1671
Size: 1363 308
BstEII G'GTnAC_C
Cuts at: 0 180 1671
Size: 180 1491
BstXI CCAn_nnnn'nTGG
Cuts at: 0 260 549 1248 1671
Size: 260 289 699 423
Fragments arranged by size:
699 423 289 260
BstYI r'GATC_y
Cuts at: 0 477 1212 1592 1671
Size: 477 735 380 79
Fragments arranged by size:
735 477 380 79
Bsu36I CC'TnA_GG
Cuts at: 0 229 1671
Size: 229 1442
BtgI C'CryG_G
Cuts at: 0 159 410 527 1179 1671
Size: 159 251 117 652 492
Fragments arranged by size:
652 492 251 159 117
BtsI GCAGTG_nn'
Cuts at: 0 218 416 436 884 1523 1671
Size: 218 198 20 448 639 148
Fragments arranged by size:
639 448 218 198 148 20
DraIII CAC_nnn'GTG
Cuts at: 0 212 1671
Size: 212 1459
DrdI GACnn_nn'nnGTC
Cuts at: 0 10 1671
Size: 10 1661
EaeI y'GGCC_r
Cuts at: 0 128 162 413 431 449 695 1565 1671
Size: 128 34 251 18 18 246 870 106
Fragments arranged by size:
870 251 246 128 106 34 18 18
EagI C'GGCC_G
Cuts at: 0 162 1671
Size: 162 1509
EarI CTCTTCn'nnn_
Cuts at: 0 74 1671
Size: 74 1597
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 934 1671
Size: 934 737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 46 259 265 388 658 1354 1466
Size: 2 44 213 6 123 270 696 112
Cuts at: 1466 1671
Size: 205
Fragments arranged by size:
696 270 213 205 123 112 44 6
2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 979 1671
Size: 979 692
EcoO109I rG'GnC_Cy
Cuts at: 0 233 234 426 466 489 1294 1329 1671
Size: 233 1 192 40 23 805 35 342
Fragments arranged by size:
805 342 233 192 40 35 23 1
FseI GG_CCGG'CC
Cuts at: 0 377 1671
Size: 377 1294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1584 1616 1671
Size: 1584 32 55
Fragments arranged by size:
1584 55 32
HincII GTy'rAC
Cuts at: 0 897 1671
Size: 897 774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1671
Size: 968 703
MscI TGG'CCA
Cuts at: 0 130 433 451 697 1567 1671
Size: 130 303 18 246 870 104
Fragments arranged by size:
870 303 246 130 104 18
MslI CAynn'nnrTG
Cuts at: 0 758 937 1246 1415 1671
Size: 758 179 309 169 256
Fragments arranged by size:
758 309 256 179 169
MspA1I CmG'CkG
Cuts at: 0 39 363 859 1671
Size: 39 324 496 812
Fragments arranged by size:
812 496 324 39
NaeI GCC'GGC
Cuts at: 0 166 375 1671
Size: 166 209 1296
Fragments arranged by size:
1296 209 166
NcoI C'CATG_G
Cuts at: 0 527 1671
Size: 527 1144
NgoMIV G'CCGG_C
Cuts at: 0 164 373 1671
Size: 164 209 1298
Fragments arranged by size:
1298 209 164
NheI G'CTAG_C
Cuts at: 0 852 1671
Size: 852 819
PasI CC'CwG_GG
Cuts at: 0 1146 1671
Size: 1146 525
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 126 462 1234 1671
Size: 42 84 336 772 437
Fragments arranged by size:
772 437 336 84 42
PfoI T'CCnGG_A
Cuts at: 0 269 395 1227 1671
Size: 269 126 832 444
Fragments arranged by size:
832 444 269 126
PpuMI rG'GwC_Cy
Cuts at: 0 426 1671
Size: 426 1245
PspOMI G'GGCC_C
Cuts at: 0 233 488 1294 1328 1671
Size: 233 255 806 34 343
Fragments arranged by size:
806 343 255 233 34
PstI C_TGCA'G
Cuts at: 0 117 423 864 1671
Size: 117 306 441 807
Fragments arranged by size:
807 441 306 117
PvuII CAG'CTG
Cuts at: 0 363 859 1671
Size: 363 496 812
Fragments arranged by size:
812 496 363
SalI G'TCGA_C
Cuts at: 0 895 1671
Size: 895 776
SfcI C'TryA_G
Cuts at: 0 113 419 860 1534 1547 1671
Size: 113 306 441 674 13 124
Fragments arranged by size:
674 441 306 124 113 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 161 1302 1671
Size: 161 1141 369
Fragments arranged by size:
1141 369 161
SmaI CCC'GGG
Cuts at: 0 627 659 1671
Size: 627 32 1012
Fragments arranged by size:
1012 627 32
StuI AGG'CCT
Cuts at: 0 403 1671
Size: 403 1268
StyI C'CwwG_G
Cuts at: 0 55 527 1413 1503 1656 1671
Size: 55 472 886 90 153 15
Fragments arranged by size:
886 472 153 90 55 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 266 1159 1423 1643 1671
Size: 266 893 264 220 28
Fragments arranged by size:
893 266 264 220 28
TatI w'GTAC_w
Cuts at: 0 1099 1671
Size: 1099 572
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 185 217 505 537 669 701 857 889
Size: 185 32 288 32 132 32 156 32
Cuts at: 889 1462 1494 1671
Size: 573 32 177
Fragments arranged by size:
573 288 185 177 156 132 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 459 1671
Size: 459 1212
XmaI C'CCGG_G
Cuts at: 0 625 657 1671
Size: 625 32 1014
Fragments arranged by size:
1014 625 32
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApaLI AseI
AvaI BanI BanII BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI
BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FseI Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NheI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SalI SfcI SfiI SmaI StuI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AsiSI AvrII BaeI BamHI
BbeI BciVI BclI BfrBI BplI BpuEI BsaAI BsaHI
BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BstBI
BstZ17I BtgZI ClaI CspCI DraI EcoICRI EcoRI EcoRV
FalI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI
ZraI