Restriction map for
pF1KB8723
(Linear) (Six-base) MAPSORT of: KIBB8723.seq Check: 303 from: 1 to: 1527
KIBB8723.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 244 1527
Size: 244 1283
AccI GT'mk_AC
Cuts at: 0 536 789 1527
Size: 536 253 738
Fragments arranged by size:
738 536 253
Acc65I G'GTAC_C
Cuts at: 0 1357 1527
Size: 1357 170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 226 658 1527
Size: 124 102 432 869
Fragments arranged by size:
869 432 124 102
AflII C'TTAA_G
Cuts at: 0 1230 1527
Size: 1230 297
AlwNI CAG_nnn'CTG
Cuts at: 0 99 204 891 1146 1527
Size: 99 105 687 255 381
Fragments arranged by size:
687 381 255 105 99
ApoI r'AATT_y
Cuts at: 0 751 1245 1527
Size: 751 494 282
Fragments arranged by size:
751 494 282
AseI AT'TA_AT
Cuts at: 0 779 1527
Size: 779 748
AvaI C'yCGr_G
Cuts at: 0 1096 1527
Size: 1096 431
BanI G'GyrC_C
Cuts at: 0 556 1357 1527
Size: 556 801 170
Fragments arranged by size:
801 556 170
BanII G_rGCy'C
Cuts at: 0 155 1103 1499 1527
Size: 155 948 396 28
Fragments arranged by size:
948 396 155 28
BciVI GTATCCnnnnn_n'
Cuts at: 0 1331 1527
Size: 1331 196
BclI T'GATC_A
Cuts at: 0 1176 1527
Size: 1176 351
BfrBI ATG'CAT
Cuts at: 0 814 1020 1527
Size: 814 206 507
Fragments arranged by size:
814 507 206
BlpI GC'TnA_GC
Cuts at: 0 455 1297 1527
Size: 455 842 230
Fragments arranged by size:
842 455 230
Bme1580I G_kGCm'C
Cuts at: 0 1510 1527
Size: 1510 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 852 1527
Size: 852 675
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 1527
Size: 1143 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 301 1123 1138 1354 1390 1527
Size: 154 147 822 15 216 36 137
Fragments arranged by size:
822 216 154 147 137 36 15
Bpu10I CC'TnA_GC
Cuts at: 0 949 1204 1399 1527
Size: 949 255 195 128
Fragments arranged by size:
949 255 195 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1527
Size: 532 995
BsaI GGTCTCn'nnnn_
Cuts at: 0 277 1527
Size: 277 1250
BsaAI yAC'GTr
Cuts at: 0 957 1314 1527
Size: 957 357 213
Fragments arranged by size:
957 357 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1396 1426 1527
Size: 1396 30 101
Fragments arranged by size:
1396 101 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 784 787 1527
Size: 784 3 740
Fragments arranged by size:
784 740 3
BseYI C'CCAG_C
Cuts at: 0 1036 1217 1429 1527
Size: 1036 181 212 98
Fragments arranged by size:
1036 212 181 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 709 1527
Size: 238 471 818
Fragments arranged by size:
818 471 238
BsiHKAI G_wGCw'C
Cuts at: 0 155 320 496 533 1527
Size: 155 165 176 37 994
Fragments arranged by size:
994 176 165 155 37
Bsp1286I G_dGCh'C
Cuts at: 0 155 320 496 533 1103 1499 1510 1527
Size: 155 165 176 37 570 396 11 17
Fragments arranged by size:
570 396 176 165 155 37 17 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 244 1527
Size: 244 1283
BsrDI GCAATG_nn'
Cuts at: 0 122 218 656 1527
Size: 122 96 438 871
Fragments arranged by size:
871 438 122 96
BsrFI r'CCGG_y
Cuts at: 0 995 1527
Size: 995 532
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 891 1527
Size: 99 792 636
Fragments arranged by size:
792 636 99
BtgI C'CryG_G
Cuts at: 0 67 1353 1527
Size: 67 1286 174
Fragments arranged by size:
1286 174 67
BtsI GCAGTG_nn'
Cuts at: 0 892 1209 1527
Size: 892 317 318
Fragments arranged by size:
892 318 317
DrdI GACnn_nn'nnGTC
Cuts at: 0 405 1527
Size: 405 1122
EaeI y'GGCC_r
Cuts at: 0 119 1527
Size: 119 1408
EarI CTCTTCn'nnn_
Cuts at: 0 160 172 836 1527
Size: 160 12 664 691
Fragments arranged by size:
691 664 160 12
EcoICRI GAG'CTC
Cuts at: 0 153 1527
Size: 153 1374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 154 226 301 658 1123 1138 1354
Size: 124 30 72 75 357 465 15 216
Cuts at: 1354 1390 1527
Size: 36 137
Fragments arranged by size:
465 357 216 137 124 75 72 36
30 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 325 1527
Size: 103 222 1202
Fragments arranged by size:
1202 222 103
EcoO109I rG'GnC_Cy
Cuts at: 0 114 1143 1527
Size: 114 1029 384
Fragments arranged by size:
1029 384 114
EcoRI G'AATT_C
Cuts at: 0 1245 1527
Size: 1245 282
EcoRV GAT'ATC
Cuts at: 0 666 1527
Size: 666 861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 316 1527
Size: 316 1211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 765 782 797 814 1527
Size: 765 17 15 17 713
Fragments arranged by size:
765 713 17 17 15
HindIII A'AGCT_T
Cuts at: 0 1233 1527
Size: 1233 294
KpnI G_GTAC'C
Cuts at: 0 1361 1527
Size: 1361 166
MscI TGG'CCA
Cuts at: 0 121 1527
Size: 121 1406
MslI CAynn'nnrTG
Cuts at: 0 46 52 809 1527
Size: 46 6 757 718
Fragments arranged by size:
757 718 46 6
MspA1I CmG'CkG
Cuts at: 0 693 1527
Size: 693 834
NaeI GCC'GGC
Cuts at: 0 997 1527
Size: 997 530
NcoI C'CATG_G
Cuts at: 0 1353 1527
Size: 1353 174
NgoMIV G'CCGG_C
Cuts at: 0 995 1527
Size: 995 532
NsiI A_TGCA'T
Cuts at: 0 816 1022 1527
Size: 816 206 505
Fragments arranged by size:
816 505 206
NspI r_CATG'y
Cuts at: 0 1393 1527
Size: 1393 134
PasI CC'CwG_GG
Cuts at: 0 146 1527
Size: 146 1381
PflMI CCAn_nnn'nTGG
Cuts at: 0 347 1146 1527
Size: 347 799 381
Fragments arranged by size:
799 381 347
PfoI T'CCnGG_A
Cuts at: 0 1139 1527
Size: 1139 388
PmlI CAC'GTG
Cuts at: 0 957 1314 1527
Size: 957 357 213
Fragments arranged by size:
957 357 213
PpuMI rG'GwC_Cy
Cuts at: 0 1143 1527
Size: 1143 384
PstI C_TGCA'G
Cuts at: 0 887 896 1283 1527
Size: 887 9 387 244
Fragments arranged by size:
887 387 244 9
PvuII CAG'CTG
Cuts at: 0 693 1527
Size: 693 834
SacI G_AGCT'C
Cuts at: 0 155 1527
Size: 155 1372
SapI GCTCTTCn'nnn_
Cuts at: 0 160 836 1527
Size: 160 676 691
Fragments arranged by size:
691 676 160
SbfI CC_TGCA'GG
Cuts at: 0 896 1527
Size: 896 631
SexAI A'CCwGG_T
Cuts at: 0 1145 1527
Size: 1145 382
SfcI C'TryA_G
Cuts at: 0 883 892 1279 1527
Size: 883 9 387 248
Fragments arranged by size:
883 387 248 9
SmlI C'TyrA_G
Cuts at: 0 547 1230 1527
Size: 547 683 297
Fragments arranged by size:
683 547 297
SphI G_CATG'C
Cuts at: 0 1393 1527
Size: 1393 134
StuI AGG'CCT
Cuts at: 0 223 1527
Size: 223 1304
StyI C'CwwG_G
Cuts at: 0 743 968 1353 1527
Size: 743 225 385 174
Fragments arranged by size:
743 385 225 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1099 1527
Size: 1099 428
TatI w'GTAC_w
Cuts at: 0 428 612 1527
Size: 428 184 915
Fragments arranged by size:
915 428 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 299 331 1396 1428 1527
Size: 299 32 1065 32 99
Fragments arranged by size:
1065 299 99 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1147 1527
Size: 1147 380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 1527
Size: 129 1398
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AlwNI ApoI AseI
AvaI BanI BanII BciVI BclI BfrBI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BstAPI BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HindIII KpnI
MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PflMI PfoI PmlI PpuMI PstI PvuII SacI
SapI SbfI SexAI SfcI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BglI BglII BmgBI BmtI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EciI FseI FspI FspAI HaeII HincII HpaI
KasI MfeI MluI MmeI NarI NdeI NheI NotI
NruI PacI PciI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmaI XmnI ZraI