Restriction map for
pF1KB8724
(Linear) (Six-base) MAPSORT of: KIBB8724.seq Check: 3986 from: 1 to: 1581
KIBB8724.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 371 1496 1581
Size: 371 1125 85
Fragments arranged by size:
1125 371 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 696 1330 1581
Size: 610 86 634 251
Fragments arranged by size:
634 610 251 86
AhdI GACnn_n'nnGTC
Cuts at: 0 378 1581
Size: 378 1203
AleI CACnn'nnGTG
Cuts at: 0 1444 1581
Size: 1444 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1162 1581
Size: 1162 419
AlwNI CAG_nnn'CTG
Cuts at: 0 400 850 1101 1581
Size: 400 450 251 480
Fragments arranged by size:
480 450 400 251
ApaLI G'TGCA_C
Cuts at: 0 613 1581
Size: 613 968
ApoI r'AATT_y
Cuts at: 0 720 988 1270 1581
Size: 720 268 282 311
Fragments arranged by size:
720 311 282 268
AvaI C'yCGr_G
Cuts at: 0 1208 1250 1581
Size: 1208 42 331
Fragments arranged by size:
1208 331 42
BamHI G'GATC_C
Cuts at: 0 449 973 1581
Size: 449 524 608
Fragments arranged by size:
608 524 449
BanI G'GyrC_C
Cuts at: 0 1429 1581
Size: 1429 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 353 1189 1581
Size: 353 836 392
Fragments arranged by size:
836 392 353
BbvCI CC'TCA_GC
Cuts at: 0 821 1581
Size: 821 760
Bme1580I G_kGCm'C
Cuts at: 0 617 1581
Size: 617 964
BmrI ACTGGGnnnn_n'
Cuts at: 0 198 988 1581
Size: 198 790 593
Fragments arranged by size:
790 593 198
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 257 1581
Size: 257 1324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 409 937 1102 1300 1581
Size: 349 60 528 165 198 281
Fragments arranged by size:
528 349 281 198 165 60
Bpu10I CC'TnA_GC
Cuts at: 0 232 821 1581
Size: 232 589 760
Fragments arranged by size:
760 589 232
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 1581
Size: 592 989
BsaAI yAC'GTr
Cuts at: 0 1131 1581
Size: 1131 450
BsaBI GATnn'nnATC
Cuts at: 0 1321 1381 1581
Size: 1321 60 200
Fragments arranged by size:
1321 200 60
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 1581
Size: 319 1262
BseYI C'CCAG_C
Cuts at: 0 64 412 1570 1581
Size: 64 348 1158 11
Fragments arranged by size:
1158 348 64 11
BsiEI CG_ry'CG
Cuts at: 0 1500 1581
Size: 1500 81
BsiHKAI G_wGCw'C
Cuts at: 0 617 1581
Size: 617 964
Bsp1286I G_dGCh'C
Cuts at: 0 617 1581
Size: 617 964
BspHI T'CATG_A
Cuts at: 0 881 1581
Size: 881 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1565 1581
Size: 1565 16
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1538 1581
Size: 1538 43
BstYI r'GATC_y
Cuts at: 0 198 449 973 1581
Size: 198 251 524 608
Fragments arranged by size:
608 524 251 198
Bsu36I CC'TnA_GG
Cuts at: 0 582 1581
Size: 582 999
BtgI C'CryG_G
Cuts at: 0 1451 1581
Size: 1451 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 580 688 1513 1581
Size: 580 108 825 68
Fragments arranged by size:
825 580 108 68
BtsI GCAGTG_nn'
Cuts at: 0 733 1581
Size: 733 848
DraI TTT'AAA
Cuts at: 0 633 1581
Size: 633 948
EaeI y'GGCC_r
Cuts at: 0 1562 1581
Size: 1562 19
EarI CTCTTCn'nnn_
Cuts at: 0 248 691 715 1551 1552 1581
Size: 248 443 24 836 1 29
Fragments arranged by size:
836 443 248 29 24 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 1581
Size: 66 1515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 409 610 696 937 1102 1300 1330
Size: 349 60 201 86 241 165 198 30
Cuts at: 1330 1581
Size: 251
Fragments arranged by size:
349 251 241 201 198 165 86 60
30
EcoO109I rG'GnC_Cy
Cuts at: 0 897 1581
Size: 897 684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1092 1581
Size: 1092 489
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 441 473 1581
Size: 364 32 45 32 1108
Fragments arranged by size:
1108 364 45 32 32
HincII GTy'rAC
Cuts at: 0 372 519 1497 1581
Size: 372 147 978 84
Fragments arranged by size:
978 372 147 84
MscI TGG'CCA
Cuts at: 0 1564 1581
Size: 1564 17
MslI CAynn'nnrTG
Cuts at: 0 132 1144 1444 1581
Size: 132 1012 300 137
Fragments arranged by size:
1012 300 137 132
MspA1I CmG'CkG
Cuts at: 0 15 156 1122 1470 1538 1581
Size: 15 141 966 348 68 43
Fragments arranged by size:
966 348 141 68 43 15
NspI r_CATG'y
Cuts at: 0 240 1149 1581
Size: 240 909 432
Fragments arranged by size:
909 432 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 287 1101 1581
Size: 287 814 480
Fragments arranged by size:
814 480 287
PstI C_TGCA'G
Cuts at: 0 14 198 1581
Size: 14 184 1383
Fragments arranged by size:
1383 184 14
PvuII CAG'CTG
Cuts at: 0 1122 1538 1581
Size: 1122 416 43
Fragments arranged by size:
1122 416 43
SalI G'TCGA_C
Cuts at: 0 370 1495 1581
Size: 370 1125 86
Fragments arranged by size:
1125 370 86
SapI GCTCTTCn'nnn_
Cuts at: 0 1552 1581
Size: 1552 29
SexAI A'CCwGG_T
Cuts at: 0 512 1581
Size: 512 1069
SfcI C'TryA_G
Cuts at: 0 10 194 1581
Size: 10 184 1387
Fragments arranged by size:
1387 184 10
SmaI CCC'GGG
Cuts at: 0 1210 1581
Size: 1210 371
SmlI C'TyrA_G
Cuts at: 0 1250 1581
Size: 1250 331
SphI G_CATG'C
Cuts at: 0 240 1149 1581
Size: 240 909 432
Fragments arranged by size:
909 432 240
StyI C'CwwG_G
Cuts at: 0 893 1067 1401 1581
Size: 893 174 334 180
Fragments arranged by size:
893 334 180 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 969 1447 1492 1581
Size: 969 478 45 89
Fragments arranged by size:
969 478 89 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 965 997 1581
Size: 965 32 584
Fragments arranged by size:
965 584 32
Tth111I GACn'n_nGTC
Cuts at: 0 367 514 1581
Size: 367 147 1067
Fragments arranged by size:
1067 367 147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 433 1581
Size: 433 1148
XhoI C'TCGA_G
Cuts at: 0 1250 1581
Size: 1250 331
XmaI C'CCGG_G
Cuts at: 0 1208 1581
Size: 1208 373
XmnI GAAnn'nnTTC
Cuts at: 0 337 988 1259 1581
Size: 337 651 271 322
Fragments arranged by size:
651 337 322 271
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AlwNI ApaLI ApoI
AvaI BamHI BanI BbsI BbvCI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI EciI Eco57MI EcoO109I
FalI Hin4I HincII MscI MslI MspA1I NspI PflMI
PstI PvuII SalI SapI SexAI SfcI SmaI SmlI
SphI StyI TaqII TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaI AscI AseI AsiSI AvrII BaeI BanII
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BpuEI BsaHI BsaWI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI DraIII
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TatI XbaI ZraI