Restriction map for
pF1KB8725
(Linear) (Six-base) MAPSORT of: KIBB8725.seq Check: 7833 from: 1 to: 1992
KIBB8725.orf
With 182 enzymes: SgfI *
July 15, 2008 09:38 ..
AccI GT'mk_AC
Cuts at: 0 428 1992
Size: 428 1564
Acc65I G'GTAC_C
Cuts at: 0 1066 1992
Size: 1066 926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1028 1788 1992
Size: 263 765 760 204
Fragments arranged by size:
765 760 263 204
AfeI AGC'GCT
Cuts at: 0 898 1992
Size: 898 1094
AleI CACnn'nnGTG
Cuts at: 0 1621 1992
Size: 1621 371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1862 1992
Size: 1862 130
AlwNI CAG_nnn'CTG
Cuts at: 0 1547 1992
Size: 1547 445
ApaLI G'TGCA_C
Cuts at: 0 873 1570 1847 1992
Size: 873 697 277 145
Fragments arranged by size:
873 697 277 145
AvaI C'yCGr_G
Cuts at: 0 489 1992
Size: 489 1503
AvrII C'CTAG_G
Cuts at: 0 1470 1992
Size: 1470 522
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1058 1091 1992
Size: 1058 33 901
Fragments arranged by size:
1058 901 33
BamHI G'GATC_C
Cuts at: 0 419 1992
Size: 419 1573
BanI G'GyrC_C
Cuts at: 0 1066 1320 1992
Size: 1066 254 672
Fragments arranged by size:
1066 672 254
BanII G_rGCy'C
Cuts at: 0 22 65 1118 1177 1265 1541 1992
Size: 22 43 1053 59 88 276 451
Fragments arranged by size:
1053 451 276 88 59 43 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 1401 1992
Size: 1401 591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1293 1327 1992
Size: 1293 34 665
Fragments arranged by size:
1293 665 34
BclI T'GATC_A
Cuts at: 0 626 998 1992
Size: 626 372 994
Fragments arranged by size:
994 626 372
BfrBI ATG'CAT
Cuts at: 0 1981 1992
Size: 1981 11
BglII A'GATC_T
Cuts at: 0 470 1992
Size: 470 1522
Bme1580I G_kGCm'C
Cuts at: 0 877 1325 1574 1851 1992
Size: 877 448 249 277 141
Fragments arranged by size:
877 448 277 249 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 278 665 1992
Size: 278 387 1327
Fragments arranged by size:
1327 387 278
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 235 823 1992
Size: 235 588 1169
Fragments arranged by size:
1169 588 235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1992
Size: 36 1956
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 809 1750 1992
Size: 46 763 941 242
Fragments arranged by size:
941 763 242 46
BsaAI yAC'GTr
Cuts at: 0 927 1495 1992
Size: 927 568 497
Fragments arranged by size:
927 568 497
BsaBI GATnn'nnATC
Cuts at: 0 850 1303 1661 1992
Size: 850 453 358 331
Fragments arranged by size:
850 453 358 331
BsaHI Gr'CG_yC
Cuts at: 0 689 1992
Size: 689 1303
BsaWI w'CCGG_w
Cuts at: 0 1653 1992
Size: 1653 339
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1627 1717 1744 1885 1992
Size: 1627 90 27 141 107
Fragments arranged by size:
1627 141 107 90 27
BseYI C'CCAG_C
Cuts at: 0 333 367 1540 1992
Size: 333 34 1173 452
Fragments arranged by size:
1173 452 333 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1351 1992
Size: 913 438 641
Fragments arranged by size:
913 641 438
BsiHKAI G_wGCw'C
Cuts at: 0 65 877 1118 1177 1265 1574 1851 1992
Size: 65 812 241 59 88 309 277 141
Fragments arranged by size:
812 309 277 241 141 88 65 59
BsmI GAATG_Cn'
Cuts at: 0 315 1007 1992
Size: 315 692 985
Fragments arranged by size:
985 692 315
Bsp1286I G_dGCh'C
Cuts at: 0 22 65 877 1118 1177 1265 1325 1541
Size: 22 43 812 241 59 88 60 216
Cuts at: 1541 1574 1851 1992
Size: 33 277 141
Fragments arranged by size:
812 277 241 216 141 88 60 59
43 33 22
BspEI T'CCGG_A
Cuts at: 0 1653 1992
Size: 1653 339
BspHI T'CATG_A
Cuts at: 0 1169 1265 1992
Size: 1169 96 727
Fragments arranged by size:
1169 727 96
BsrDI GCAATG_nn'
Cuts at: 0 78 1397 1992
Size: 78 1319 595
Fragments arranged by size:
1319 595 78
BsrGI T'GTAC_A
Cuts at: 0 1013 1992
Size: 1013 979
BssSI C'ACGA_G
Cuts at: 0 58 1992
Size: 58 1934
BstAPI GCAn_nnn'nTGC
Cuts at: 0 562 1987 1992
Size: 562 1425 5
Fragments arranged by size:
1425 562 5
BstEII G'GTnAC_C
Cuts at: 0 36 1956 1992
Size: 36 1920 36
Fragments arranged by size:
1920 36 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1386 1992
Size: 1386 606
BstYI r'GATC_y
Cuts at: 0 419 470 801 1992
Size: 419 51 331 1191
Fragments arranged by size:
1191 419 331 51
Bsu36I CC'TnA_GG
Cuts at: 0 394 516 1751 1992
Size: 394 122 1235 241
Fragments arranged by size:
1235 394 241 122
BtgI C'CryG_G
Cuts at: 0 32 823 1217 1961 1992
Size: 32 791 394 744 31
Fragments arranged by size:
791 744 394 32 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 270 1074 1296 1992
Size: 270 804 222 696
Fragments arranged by size:
804 696 270 222
BtsI GCAGTG_nn'
Cuts at: 0 560 1992
Size: 560 1432
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1088 1123 1992
Size: 1088 35 869
Fragments arranged by size:
1088 869 35
DraIII CAC_nnn'GTG
Cuts at: 0 252 675 1345 1992
Size: 252 423 670 647
Fragments arranged by size:
670 647 423 252
EaeI y'GGCC_r
Cuts at: 0 119 202 243 260 1992
Size: 119 83 41 17 1732
Fragments arranged by size:
1732 119 83 41 17
EarI CTCTTCn'nnn_
Cuts at: 0 1216 1360 1426 1603 1721 1862 1992
Size: 1216 144 66 177 118 141 130
Fragments arranged by size:
1216 177 144 141 130 118 66
EcoICRI GAG'CTC
Cuts at: 0 63 1116 1175 1263 1992
Size: 63 1053 59 88 729
Fragments arranged by size:
1053 729 88 63 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 263 1028 1788 1992
Size: 36 227 765 760 204
Fragments arranged by size:
765 760 227 204 36
EcoO109I rG'GnC_Cy
Cuts at: 0 585 1747 1992
Size: 585 1162 245
Fragments arranged by size:
1162 585 245
EcoRV GAT'ATC
Cuts at: 0 84 1992
Size: 84 1908
FspI TGC'GCA
Cuts at: 0 1690 1992
Size: 1690 302
HaeII r_GCGC'y
Cuts at: 0 900 1202 1992
Size: 900 302 790
Fragments arranged by size:
900 790 302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 1992
Size: 472 32 1488
Fragments arranged by size:
1488 472 32
KpnI G_GTAC'C
Cuts at: 0 1070 1992
Size: 1070 922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 144 745 1123 1660 1992
Size: 105 39 601 378 537 332
Fragments arranged by size:
601 537 378 332 105 39
MscI TGG'CCA
Cuts at: 0 245 1992
Size: 245 1747
MslI CAynn'nnrTG
Cuts at: 0 634 982 985 1621 1885 1954 1992
Size: 634 348 3 636 264 69 38
Fragments arranged by size:
636 634 348 264 69 38 3
MspA1I CmG'CkG
Cuts at: 0 34 417 1992
Size: 34 383 1575
Fragments arranged by size:
1575 383 34
NcoI C'CATG_G
Cuts at: 0 823 1217 1992
Size: 823 394 775
Fragments arranged by size:
823 775 394
NsiI A_TGCA'T
Cuts at: 0 1983 1992
Size: 1983 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 829 1992
Size: 252 577 1163
Fragments arranged by size:
1163 577 252
PmlI CAC'GTG
Cuts at: 0 927 1992
Size: 927 1065
SacI G_AGCT'C
Cuts at: 0 65 1118 1177 1265 1992
Size: 65 1053 59 88 727
Fragments arranged by size:
1053 727 88 65 59
SacII CC_GC'GG
Cuts at: 0 35 1992
Size: 35 1957
SspI AAT'ATT
Cuts at: 0 1060 1992
Size: 1060 932
StuI AGG'CCT
Cuts at: 0 521 1139 1992
Size: 521 618 853
Fragments arranged by size:
853 618 521
StyI C'CwwG_G
Cuts at: 0 772 823 1070 1217 1232 1470 1992
Size: 772 51 247 147 15 238 522
Fragments arranged by size:
772 522 247 238 147 51 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1308 1410 1866 1992
Size: 1308 102 456 126
Fragments arranged by size:
1308 456 126 102
TatI w'GTAC_w
Cuts at: 0 704 1013 1580 1992
Size: 704 309 567 412
Fragments arranged by size:
704 567 412 309
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 978 1454 1992
Size: 978 476 538
Fragments arranged by size:
978 538 476
Enzymes that do cut:
AccI Acc65I AcuI AfeI AleI AlfI AlwNI ApaLI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BclI BfrBI BglII Bme1580I BmrI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrDI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FspI
HaeII Hin4I KpnI MmeI MscI MslI MspA1I NcoI
NsiI PflMI PmlI SacI SacII SspI StuI StyI
TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AloI
ApaI ApoI AscI AseI AsiSI BbeI BbvCI BciVI
BglI BlpI BmgBI BmtI Bpu10I BpuEI BsaXI BsiEI
BsiWI BsmBI BspMI BsrBI BsrFI BssHII BstBI BstZ17I
ClaI DraI DrdI EagI EciI EcoNI EcoRI FalI
FseI FspAI HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI