Restriction map for
pF1KB8727
(Linear) (Six-base) MAPSORT of: KIBB8727.seq Check: 5225 from: 1 to: 1476
KIBB8727.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 1080 1476
Size: 1080 396
AflIII A'CryG_T
Cuts at: 0 377 1100 1476
Size: 377 723 376
Fragments arranged by size:
723 377 376
AhdI GACnn_n'nnGTC
Cuts at: 0 474 1476
Size: 474 1002
AleI CACnn'nnGTG
Cuts at: 0 334 1279 1476
Size: 334 945 197
Fragments arranged by size:
945 334 197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1018 1050 1476
Size: 1018 32 426
Fragments arranged by size:
1018 426 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1065 1269 1476
Size: 1065 204 207
Fragments arranged by size:
1065 207 204
ApaI G_GGCC'C
Cuts at: 0 93 979 1402 1476
Size: 93 886 423 74
Fragments arranged by size:
886 423 93 74
ApoI r'AATT_y
Cuts at: 0 1324 1333 1476
Size: 1324 9 143
Fragments arranged by size:
1324 143 9
AvaI C'yCGr_G
Cuts at: 0 1404 1476
Size: 1404 72
BanI G'GyrC_C
Cuts at: 0 245 723 1476
Size: 245 478 753
Fragments arranged by size:
753 478 245
BanII G_rGCy'C
Cuts at: 0 93 743 770 812 979 1036 1194 1402
Size: 93 650 27 42 167 57 158 208
Cuts at: 1402 1476
Size: 74
Fragments arranged by size:
650 208 167 158 93 74 57 42
27
BbsI GAAGACnn'nnnn_
Cuts at: 0 1444 1476
Size: 1444 32
BbvCI CC'TCA_GC
Cuts at: 0 1389 1394 1476
Size: 1389 5 82
Fragments arranged by size:
1389 82 5
BglI GCCn_nnn'nGGC
Cuts at: 0 952 1476
Size: 952 524
BglII A'GATC_T
Cuts at: 0 104 1476
Size: 104 1372
Bme1580I G_kGCm'C
Cuts at: 0 93 248 547 728 979 1402 1476
Size: 93 155 299 181 251 423 74
Fragments arranged by size:
423 299 251 181 155 93 74
BmgBI CAC'GTC
Cuts at: 0 1281 1476
Size: 1281 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 1222 1476
Size: 96 1126 254
Fragments arranged by size:
1126 254 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 466 537 691 772 826 1020 1087
Size: 17 449 71 154 81 54 194 67
Cuts at: 1087 1208 1365 1476
Size: 121 157 111
Fragments arranged by size:
449 194 157 154 121 111 81 71
67 54 17
Bpu10I CC'TnA_GC
Cuts at: 0 1389 1394 1476
Size: 1389 5 82
Fragments arranged by size:
1389 82 5
BsaAI yAC'GTr
Cuts at: 0 258 779 1476
Size: 258 521 697
Fragments arranged by size:
697 521 258
BsaHI Gr'CG_yC
Cuts at: 0 1199 1476
Size: 1199 277
BsaWI w'CCGG_w
Cuts at: 0 613 1476
Size: 613 863
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1018 1048 1476
Size: 1018 30 428
Fragments arranged by size:
1018 428 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 859 1476
Size: 859 617
BseYI C'CCAG_C
Cuts at: 0 984 1010 1422 1476
Size: 984 26 412 54
Fragments arranged by size:
984 412 54 26
BsiHKAI G_wGCw'C
Cuts at: 0 353 770 812 1216 1476
Size: 353 417 42 404 260
Fragments arranged by size:
417 404 353 260 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 118 1476
Size: 118 1358
Bsp1286I G_dGCh'C
Cuts at: 0 93 248 353 547 728 743 770 812
Size: 93 155 105 194 181 15 27 42
Cuts at: 812 979 1036 1194 1216 1402 1476
Size: 167 57 158 22 186 74
Fragments arranged by size:
194 186 181 167 158 155 105 93
74 57 42 27 22 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 1476
Size: 437 1039
BsrFI r'CCGG_y
Cuts at: 0 951 994 1476
Size: 951 43 482
Fragments arranged by size:
951 482 43
BssSI C'ACGA_G
Cuts at: 0 193 1476
Size: 193 1283
BstEII G'GTnAC_C
Cuts at: 0 411 1311 1476
Size: 411 900 165
Fragments arranged by size:
900 411 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1281 1476
Size: 1281 195
BstYI r'GATC_y
Cuts at: 0 104 1476
Size: 104 1372
Bsu36I CC'TnA_GG
Cuts at: 0 1304 1476
Size: 1304 172
BtgI C'CryG_G
Cuts at: 0 1358 1476
Size: 1358 118
BtsI GCAGTG_nn'
Cuts at: 0 669 1047 1270 1476
Size: 669 378 223 206
Fragments arranged by size:
669 378 223 206
DraIII CAC_nnn'GTG
Cuts at: 0 198 1476
Size: 198 1278
EaeI y'GGCC_r
Cuts at: 0 266 573 1476
Size: 266 307 903
Fragments arranged by size:
903 307 266
EarI CTCTTCn'nnn_
Cuts at: 0 160 1292 1476
Size: 160 1132 184
Fragments arranged by size:
1132 184 160
EcoICRI GAG'CTC
Cuts at: 0 768 810 1476
Size: 768 42 666
Fragments arranged by size:
768 666 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 466 537 691 772 826 1020 1087
Size: 17 449 71 154 81 54 194 67
Cuts at: 1087 1208 1365 1476
Size: 121 157 111
Fragments arranged by size:
449 194 157 154 121 111 81 71
67 54 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1249 1476
Size: 1249 227
EcoO109I rG'GnC_Cy
Cuts at: 0 90 136 426 801 933 976 1398 1399
Size: 90 46 290 375 132 43 422 1
Cuts at: 1399 1408 1476
Size: 9 68
Fragments arranged by size:
422 375 290 132 90 68 46 43
9 1
FspI TGC'GCA
Cuts at: 0 661 1476
Size: 661 815
HaeII r_GCGC'y
Cuts at: 0 1429 1476
Size: 1429 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 192 224 460 492 508 540
Size: 32 32 128 32 236 32 16 32
Cuts at: 540 1476
Size: 936
Fragments arranged by size:
936 236 128 32 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 1081 1476
Size: 1081 395
MluI A'CGCG_T
Cuts at: 0 377 1476
Size: 377 1099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1476
Size: 683 793
MscI TGG'CCA
Cuts at: 0 268 575 1476
Size: 268 307 901
Fragments arranged by size:
901 307 268
MslI CAynn'nnrTG
Cuts at: 0 334 1279 1476
Size: 334 945 197
Fragments arranged by size:
945 334 197
MspA1I CmG'CkG
Cuts at: 0 798 837 958 1014 1360 1393 1476
Size: 798 39 121 56 346 33 83
Fragments arranged by size:
798 346 121 83 56 39 33
NaeI GCC'GGC
Cuts at: 0 953 1476
Size: 953 523
NgoMIV G'CCGG_C
Cuts at: 0 951 1476
Size: 951 525
NspI r_CATG'y
Cuts at: 0 474 583 1104 1464 1476
Size: 474 109 521 360 12
Fragments arranged by size:
521 474 360 109 12
PciI A'CATG_T
Cuts at: 0 1100 1476
Size: 1100 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 261 1476
Size: 261 1215
PfoI T'CCnGG_A
Cuts at: 0 803 1337 1476
Size: 803 534 139
Fragments arranged by size:
803 534 139
PmlI CAC'GTG
Cuts at: 0 258 779 1476
Size: 258 521 697
Fragments arranged by size:
697 521 258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1018 1050 1476
Size: 1018 32 426
Fragments arranged by size:
1018 426 32
PpuMI rG'GwC_Cy
Cuts at: 0 136 426 801 1408 1476
Size: 136 290 375 607 68
Fragments arranged by size:
607 375 290 136 68
PspOMI G'GGCC_C
Cuts at: 0 89 975 1398 1476
Size: 89 886 423 78
Fragments arranged by size:
886 423 89 78
PstI C_TGCA'G
Cuts at: 0 77 764 1061 1476
Size: 77 687 297 415
Fragments arranged by size:
687 415 297 77
PvuII CAG'CTG
Cuts at: 0 837 958 1014 1393 1476
Size: 837 121 56 379 83
Fragments arranged by size:
837 379 121 83 56
RsrII CG'GwC_CG
Cuts at: 0 1362 1476
Size: 1362 114
SacI G_AGCT'C
Cuts at: 0 770 812 1476
Size: 770 42 664
Fragments arranged by size:
770 664 42
SacII CC_GC'GG
Cuts at: 0 1361 1476
Size: 1361 115
SalI G'TCGA_C
Cuts at: 0 1079 1476
Size: 1079 397
SanDI GG'GwC_CC
Cuts at: 0 1408 1476
Size: 1408 68
SapI GCTCTTCn'nnn_
Cuts at: 0 1292 1476
Size: 1292 184
SfcI C'TryA_G
Cuts at: 0 73 760 1057 1476
Size: 73 687 297 419
Fragments arranged by size:
687 419 297 73
SmaI CCC'GGG
Cuts at: 0 1406 1476
Size: 1406 70
SphI G_CATG'C
Cuts at: 0 583 1476
Size: 583 893
StuI AGG'CCT
Cuts at: 0 443 1072 1240 1476
Size: 443 629 168 236
Fragments arranged by size:
629 443 236 168
StyI C'CwwG_G
Cuts at: 0 929 1476
Size: 929 547
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 342 495 745 1249 1379 1476
Size: 342 153 250 504 130 97
Fragments arranged by size:
504 342 250 153 130 97
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 462 1476
Size: 462 1014
XmaI C'CCGG_G
Cuts at: 0 1404 1476
Size: 1404 72
XmnI GAAnn'nnTTC
Cuts at: 0 22 1476
Size: 22 1454
Enzymes that do cut:
AccI AflIII AhdI AleI AloI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BbvCI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII MluI MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII RsrII SacI SacII SalI SanDI SapI
SfcI SmaI SphI StuI StyI TaqII XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BlpI BmtI BplI
BpuEI BsaI BsaBI BsgI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI
MfeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PshAI PsiI PspXI PsrI PvuI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI ZraI