Restriction map for
pF1KB8728
(Linear) (Six-base) MAPSORT of: KIBB8728.seq Check: 2512 from: 1 to: 1116
KIBB8728.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AccI GT'mk_AC
Cuts at: 0 502 962 1116
Size: 502 460 154
Fragments arranged by size:
502 460 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1116
Size: 637 479
AleI CACnn'nnGTG
Cuts at: 0 469 1116
Size: 469 647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1097 1116
Size: 1097 19
AlwNI CAG_nnn'CTG
Cuts at: 0 140 498 753 1116
Size: 140 358 255 363
Fragments arranged by size:
363 358 255 140
ApaLI G'TGCA_C
Cuts at: 0 472 1116
Size: 472 644
ApoI r'AATT_y
Cuts at: 0 786 1116
Size: 786 330
BanI G'GyrC_C
Cuts at: 0 261 1116
Size: 261 855
BanII G_rGCy'C
Cuts at: 0 20 1116
Size: 20 1096
BbsI GAAGACnn'nnnn_
Cuts at: 0 101 625 712 1040 1116
Size: 101 524 87 328 76
Fragments arranged by size:
524 328 101 87 76
BbvCI CC'TCA_GC
Cuts at: 0 881 1116
Size: 881 235
BglII A'GATC_T
Cuts at: 0 72 803 1116
Size: 72 731 313
Fragments arranged by size:
731 313 72
Bme1580I G_kGCm'C
Cuts at: 0 266 398 476 1116
Size: 266 132 78 640
Fragments arranged by size:
640 266 132 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 382 646 914 1116
Size: 340 42 264 268 202
Fragments arranged by size:
340 268 264 202 42
Bpu10I CC'TnA_GC
Cuts at: 0 881 1116
Size: 881 235
BsaAI yAC'GTr
Cuts at: 0 930 1116
Size: 930 186
BsaHI Gr'CG_yC
Cuts at: 0 632 1116
Size: 632 484
BsaWI w'CCGG_w
Cuts at: 0 946 968 1116
Size: 946 22 148
Fragments arranged by size:
946 148 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 300 898 1116
Size: 300 598 218
Fragments arranged by size:
598 300 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1116
Size: 622 494
BsiHKAI G_wGCw'C
Cuts at: 0 476 1116
Size: 476 640
BsmI GAATG_Cn'
Cuts at: 0 978 1116
Size: 978 138
Bsp1286I G_dGCh'C
Cuts at: 0 20 266 398 476 1116
Size: 20 246 132 78 640
Fragments arranged by size:
640 246 132 78 20
BspEI T'CCGG_A
Cuts at: 0 968 1116
Size: 968 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 196 1116
Size: 196 920
BsrFI r'CCGG_y
Cuts at: 0 338 1116
Size: 338 778
BstYI r'GATC_y
Cuts at: 0 72 803 848 1116
Size: 72 731 45 268
Fragments arranged by size:
731 268 72 45
BstZ17I GTA'TAC
Cuts at: 0 503 1116
Size: 503 613
Bsu36I CC'TnA_GG
Cuts at: 0 22 1116
Size: 22 1094
BtgI C'CryG_G
Cuts at: 0 545 711 1116
Size: 545 166 405
Fragments arranged by size:
545 405 166
BtsI GCAGTG_nn'
Cuts at: 0 356 639 1116
Size: 356 283 477
Fragments arranged by size:
477 356 283
DraIII CAC_nnn'GTG
Cuts at: 0 480 1116
Size: 480 636
EarI CTCTTCn'nnn_
Cuts at: 0 103 244 1116
Size: 103 141 872
Fragments arranged by size:
872 141 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 382 637 646 914 1116
Size: 340 42 255 9 268 202
Fragments arranged by size:
340 268 255 202 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 451 1116
Size: 451 665
EcoO109I rG'GnC_Cy
Cuts at: 0 137 495 1116
Size: 137 358 621
Fragments arranged by size:
621 358 137
EcoRI G'AATT_C
Cuts at: 0 786 1116
Size: 786 330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 314 540 1116
Size: 314 226 576
Fragments arranged by size:
576 314 226
FspI TGC'GCA
Cuts at: 0 926 1116
Size: 926 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 764 796 874 906 1116
Size: 589 32 143 32 78 32 210
Fragments arranged by size:
589 210 143 78 32 32 32
HincII GTy'rAC
Cuts at: 0 537 1116
Size: 537 579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1116
Size: 432 684
MslI CAynn'nnrTG
Cuts at: 0 469 956 1105 1116
Size: 469 487 149 11
Fragments arranged by size:
487 469 149 11
NaeI GCC'GGC
Cuts at: 0 340 1116
Size: 340 776
NcoI C'CATG_G
Cuts at: 0 545 1116
Size: 545 571
NgoMIV G'CCGG_C
Cuts at: 0 338 1116
Size: 338 778
NspI r_CATG'y
Cuts at: 0 573 1116
Size: 573 543
PasI CC'CwG_GG
Cuts at: 0 134 266 656 1116
Size: 134 132 390 460
Fragments arranged by size:
460 390 134 132
PflMI CCAn_nnn'nTGG
Cuts at: 0 140 834 1116
Size: 140 694 282
Fragments arranged by size:
694 282 140
PmlI CAC'GTG
Cuts at: 0 930 1116
Size: 930 186
PshAI GACnn'nnGTC
Cuts at: 0 947 1116
Size: 947 169
PstI C_TGCA'G
Cuts at: 0 383 494 1116
Size: 383 111 622
Fragments arranged by size:
622 383 111
SfcI C'TryA_G
Cuts at: 0 379 490 1116
Size: 379 111 626
Fragments arranged by size:
626 379 111
SphI G_CATG'C
Cuts at: 0 573 1116
Size: 573 543
SspI AAT'ATT
Cuts at: 0 1014 1116
Size: 1014 102
StyI C'CwwG_G
Cuts at: 0 545 1116
Size: 545 571
TatI w'GTAC_w
Cuts at: 0 818 1116
Size: 818 298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1017 1116
Size: 1017 99
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaLI ApoI BanI
BanII BbsI BbvCI BglII Bme1580I BpmI Bpu10I BsaAI
BsaHI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrFI BstYI BstZ17I Bsu36I BtgI BtsI DraIII
EarI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I
HincII MmeI MslI NaeI NcoI NgoMIV NspI PasI
PflMI PmlI PshAI PstI SfcI SphI SspI StyI
TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI
BsaXI BseYI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BtgZI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoRV FseI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI MspA1I NarI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
XmnI ZraI